DOI: 10.1261/rna.2860603. RNAi: Mammalian oocytes do it without RNA-dependent RNA polymerase

RNAi: Mammalian oocytes do it without RNA-dependent RNA polymerase

10.1261/rna.2860603

Crossref journal-article

Cold Spring Harbor Laboratory

RNA (246)

Abstract

Studies in mutant organisms deficient in RNA interference (RNAi) and related post-transcriptional gene silencing implicated a role for a single class of RNA-dependent RNA polymerases (RdRp). Nevertheless, sequence homologs to these RdRps have not been found in coelomate organisms such as Drosophila or mammals. This lack of homologous sequences does not exclude that an RdRp functions in RNAi in these organisms because an RdRp could be acquired by horizontal transfer from an RNA virus. In fact, such a sequence is found in mice (Aquarius) and we observe that it is expressed in mouse oocytes and early embryos, which exhibit RNAi. We report here that cordycepin, an inhibitor of RNA synthesis, does not prevent Mos double-strand RNA (dsRNA) to target endogenous Mos mRNA in mouse oocytes and that targeting a chimeric Mos–EGFP mRNA with dsRNA to EGFP does not reduce the endogenous Mos mRNA, but does target the chimeric mRNA. These results indicate that an RdRp is not involved in dsRNA-mediated mRNA degradation in mammalian oocytes, and possibly in mammals in general, and therefore that only homologous sequences to the dsRNA are targeted for degradation.

Bibliography

STEIN, P., SVOBODA, P., ANGER, M., & SCHULTZ, R. M. (2003). RNAi: Mammalian oocytes do it without RNA-dependent RNA polymerase. RNA, 9(2), 187â192.

Authors 4

PAULA STEIN (first)
PETR SVOBODA (additional)
MARTIN ANGER (additional)
RICHARD M. SCHULTZ (additional)

References 27 Referenced 93

10.1038/35053110
10.1073/pnas.261562698
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10.1017/S1355838202021064
10.1530/jrf.0.0860679
10.1038/20215
10.1016/S0092-8674(00)80864-8
10.1023/A:1015687008357 / Transgenic Res. (2002)
10.1095/biolreprod65.4.986
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10.1242/dev.127.19.4147 / Development (2000)
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10.1016/S0014-5793(00)01883-4
{'key': '2021112105330860000_9.2.187.26', 'first-page': '129', 'volume': '6', 'year': '1971', 'journal-title': 'Adv. Biosci.'} / Adv. Biosci. (1971)
10.1016/S0092-8674(00)80620-0

Dates

Type	When
Created	22 years, 7 months ago (Jan. 21, 2003, 8:24 p.m.)
Deposited	3 years, 9 months ago (Nov. 21, 2021, 8:34 a.m.)
Indexed	1 year ago (Aug. 7, 2024, 10:17 p.m.)
Issued	22 years, 7 months ago (Feb. 1, 2003)
Published	22 years, 7 months ago (Feb. 1, 2003)
Published Online	22 years, 7 months ago (Feb. 1, 2003)
Published Print	22 years, 7 months ago (Feb. 1, 2003)

Funders 0

None

BibTeX

@article{STEIN_2003, title={RNAi: Mammalian oocytes do it without RNA-dependent RNA polymerase}, volume={9}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.2860603}, DOI={10.1261/rna.2860603}, number={2}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={STEIN, PAULA and SVOBODA, PETR and ANGER, MARTIN and SCHULTZ, RICHARD M.}, year={2003}, month=feb, pages={187–192} }

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