Antagonistic regulation of α-actinin alternative splicing by CELF proteins and polypyrimidine tract binding protein
10.1261/rna.2191903
Crossref journal-article
Cold Spring Harbor Laboratory
RNA (246)
Abstract

The α-actinin gene has a pair of alternatively spliced exons. The smooth muscle (SM) exon is repressed in most cell types by polypyrimidine tract binding protein (PTB). CELF (CUG-BP and ETR3-like factors) family proteins, splicing regulators whose activities are altered in myotonic dystrophy, were found to coordinately regulate selection of the two α-actinin exons. CUG-BP and ETR3 activated the SM exon, and along with CELF4 they were also able to repress splicing of the NM (nonmuscle) exon both in vivo and in vitro. Activation of SM exon splicing was associated with displacement of PTB from the polypyrimidine tract by binding of CUG-BP at adjacent sites. Our data provides direct evidence for the activity of CELF proteins as both activators and repressors of splicing within a single-model system of alternative splicing, and suggests a model whereby α-actinin alternative splicing is regulated by synergistic and antagonistic interactions between members of the CELF and PTB families.

Bibliography

GROMAK, N., MATLIN, A. J., COOPER, T. A., & SMITH, C. W. J. (2003). Antagonistic regulation of α-actinin alternative splicing by CELF proteins and polypyrimidine tract binding protein. RNA, 9(4), 443–456.

Authors 4
  1. NATALIA GROMAK (first)
  2. ARIANNE J. MATLIN (additional)
  3. THOMAS A. COOPER (additional)
  4. CHRISTOPHER W.J. SMITH (additional)
References 58 Referenced 84
  1. 10.1016/S0092-8674(00)00128-8
  2. 10.1016/S0968-0004(00)01549-8
  3. Burge, C., Tuschl, T., and Sharp, P. 1999. Splicing of precursors to mRNAs by the spliceosomes. In The RNA world, 2nd ed. (eds. R.F. Gestetland et al.), pp. 525–560. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
  4. 10.1016/S0168-9525(01)02626-9
  5. 10.1126/science.8085156
  6. 10.1093/emboj/18.14.4060
  7. 10.1038/nrg775
  8. 10.1016/S1097-2765(02)00479-3
  9. 10.1016/S1097-2765(02)00572-5
  10. 10.1016/S0378-1119(99)00312-1
  11. 10.1016/S1097-2765(00)80260-9
  12. 10.1128/MCB.18.8.4519 / Mol. Cell. Biol. (1998)
  13. 10.1128/MCB.19.1.251 / Mol. Cell. Biol. (1999)
  14. {'key': '2021112105331862000_9.4.443.14', 'first-page': '663', 'volume': '1', 'year': '1995', 'journal-title': 'RNA'} / RNA (1995)
  15. 10.1074/jbc.M003083200
  16. 10.1002/j.1460-2075.1994.tb06697.x / EMBO J. (1994)
  17. {'key': '2021112105331862000_9.4.443.17', 'first-page': '85', 'volume': '4', 'year': '1998', 'journal-title': 'RNA'} / RNA (1998)
  18. 10.1017/S1355838200000960
  19. 10.1016/S0168-9525(00)02176-4
  20. 10.1093/nar/gkf480
  21. 10.1101/gad.13.4.437 / Genes & Dev. (1999)
  22. 10.1016/S0896-6273(00)80900-9
  23. 10.1016/S0955-0674(99)80051-9
  24. 10.1016/S0006-291X(02)00734-9
  25. 10.1128/MCB.21.4.1285-1296.2001
  26. {'key': '2021112105331862000_9.4.443.26', 'first-page': '234', 'volume': '1', 'year': '1995', 'journal-title': 'RNA'} / RNA (1995)
  27. 10.1146/annurev.genet.32.1.279
  28. 10.1093/hmg/8.1.53
  29. 10.1038/418236a
  30. 10.1016/S1097-2765(02)00563-4
  31. 10.1128/MCB.20.20.7463-7479.2000
  32. McAfee, J.G., Huang, M., Soltaninassab, S., Rech, J.E., Iyengar, S., and Lesturgeon, W.M. 1997. The packaging of pre-mRNA. In Eukaryotic mRNA processing (ed. A.R. Krainer), pp. 68–102. Oxford University Press, Oxford/New York. (10.1093/oso/9780199634187.003.0003)
  33. 10.1038/ng0102-13
  34. Moore, M.J. and Query, C.C. 1998. Uses of site-specifically modified RNAs constructed by RNA ligation. In RNA protein interactions: A practical approach (ed. C.W.J. Smith), Vol. 192, pp. 75–108. Oxford University Press, Oxford. (10.1093/oso/9780199636518.003.0003)
  35. {'key': '2021112105331862000_9.4.443.35', 'first-page': '764', 'volume': '3', 'year': '1997', 'journal-title': 'RNA'} / RNA (1997)
  36. 10.1126/science.280.5364.737
  37. 10.1073/pnas.110128397
  38. 10.1016/S0955-0674(00)00097-1
  39. 10.1016/S1367-5931(02)00320-4
  40. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
  41. 10.1038/ng704
  42. 10.1002/j.1460-2075.1995.tb07326.x / EMBO J. (1995)
  43. 10.1126/science.7761834
  44. 10.1016/S0968-0004(00)01604-2
  45. Smith, C.W.J., Gooding, C., Roberts, G.C., and Scadden, A.D.J. 1996. Analyzing patterns of alternative splicing. In A laboratory guide to RNA. Isolation, analysis and synthesis (ed. P.A. Kreig), pp. 411–440. John Wiley & Sons, Inc., New York.
  46. 10.1128/MCB.19.4.2699 / Mol. Cell. Biol. (1999)
  47. 10.1046/j.1356-9597.2001.00506.x
  48. 10.1006/bbrc.2000.3694
  49. 10.1093/nar/24.22.4407
  50. 10.1093/hmg/5.1.115
  51. 10.1016/S0960-9822(06)00361-7
  52. 10.1128/MCB.21.10.3281-3288.2001
  53. 10.1016/S1097-2765(02)00645-7
  54. 10.1016/S0021-9258(18)42690-7 / J. Biol. Chem. (1992)
  55. 10.1017/S1355838201010214
  56. 10.1017/S1355838299981530
  57. 10.1017/S1355838202027036
  58. 10.1016/S1097-2765(01)00409-9
Dates
Type When
Created 22 years, 5 months ago (March 20, 2003, 5:44 p.m.)
Deposited 1 year, 7 months ago (Jan. 9, 2024, 9:16 a.m.)
Indexed 1 year ago (Aug. 10, 2024, 3:09 a.m.)
Issued 22 years, 5 months ago (April 1, 2003)
Published 22 years, 5 months ago (April 1, 2003)
Published Online 22 years, 5 months ago (April 1, 2003)
Published Print 22 years, 5 months ago (April 1, 2003)
Funders 0

None

@article{GROMAK_2003, title={Antagonistic regulation of α-actinin alternative splicing by CELF proteins and polypyrimidine tract binding protein}, volume={9}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.2191903}, DOI={10.1261/rna.2191903}, number={4}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={GROMAK, NATALIA and MATLIN, ARIANNE J. and COOPER, THOMAS A. and SMITH, CHRISTOPHER W.J.}, year={2003}, month=apr, pages={443–456} }