DOI: 10.1261/rna.2119903. Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors

Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors

10.1261/rna.2119903

Crossref journal-article

Cold Spring Harbor Laboratory

RNA (246)

Abstract

Through exhaustive two-hybrid screens using a budding yeast genomic library, and starting with the splicing factor and DEAH-box RNA helicase Prp22p as bait, we identified yeast Prp45p and Prp46p. We show that as well as interacting in two-hybrid screens, Prp45p and Prp46p interact with each other in vitro. We demonstrate that Prp45p and Prp46p are spliceosome associated throughout the splicing process and both are essential for pre-mRNA splicing. Under nonsplicing conditions they also associate in coprecipitation assays with low levels of the U2, U5, and U6 snRNAs that may indicate their presence in endogenous activated spliceosomes or in a postsplicing snRNP complex.

Bibliography

ALBERS, M., DIMENT, A., MURARU, M., RUSSELL, C. S., & BEGGS, J. D. (2003). Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors. RNA, 9(1), 138â150.

Authors 5

MICHAEL ALBERS (first)
ANN DIMENT (additional)
MARIELA MURARU (additional)
CAROLINE S. RUSSELL (additional)
JEAN D. BEGGS (additional)

References 53 Referenced 53

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Dates

Type	When
Created	22 years, 7 months ago (Jan. 16, 2003, 6:27 p.m.)
Deposited	1 year, 7 months ago (Jan. 8, 2024, 2:56 p.m.)
Indexed	2 months, 1 week ago (June 20, 2025, 8:05 p.m.)
Issued	22 years, 7 months ago (Jan. 1, 2003)
Published	22 years, 7 months ago (Jan. 1, 2003)
Published Online	22 years, 7 months ago (Jan. 1, 2003)
Published Print	22 years, 7 months ago (Jan. 1, 2003)

Funders 0

None

BibTeX

@article{ALBERS_2003, title={Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors}, volume={9}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.2119903}, DOI={10.1261/rna.2119903}, number={1}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={ALBERS, MICHAEL and DIMENT, ANN and MURARU, MARIELA and RUSSELL, CAROLINE S. and BEGGS, JEAN D.}, year={2003}, month=jan, pages={138–150} }

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