DOI: 10.1261/rna.2030510. Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing

Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing

10.1261/rna.2030510

Crossref journal-article

Cold Spring Harbor Laboratory

RNA (246)

Abstract

Eukaryotic pre-mRNA splicing is a complex process requiring the precise timing and action of >100 trans-acting factors. It has been known for some time that the two steps of splicing chemistry require three DEAH-box RNA helicase-like proteins; however, their mechanism of action at these steps has remained elusive. Spliceosomes arrested in vivo at the three helicase checkpoints were purified, and first step-arrested spliceosomes were functionally characterized. We show that the first step of splicing requires a novel ATP-independent conformational change. Prp2p then catalyzes an ATP-dependent rearrangement displacing the SF3a and SF3b complexes from the branchpoint within the spliceosome. We propose a model in which SF3 prevents premature nucleophilic attack of the chemically reactive hydroxyl of the branchpoint adenosine prior to the first transesterification. When the spliceosome attains the proper conformation and upon the function of Prp2p, SF3 is displaced from the branchpoint allowing first step chemistry to occur.

Bibliography

Lardelli, R. M., Thompson, J. X., Yates, J. R., & Stevens, S. W. (2010). Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing. RNA, 16(3), 516â528.

Authors 4

Rea M. Lardelli (first)
James X. Thompson (additional)
John R. Yates (additional)
Scott W. Stevens (additional)

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Dates

Type	When
Created	15 years, 7 months ago (Jan. 20, 2010, 9:51 p.m.)
Deposited	3 years, 9 months ago (Nov. 21, 2021, 8:59 a.m.)
Indexed	1 month ago (July 27, 2025, 3:40 a.m.)
Issued	15 years, 7 months ago (Jan. 20, 2010)
Published	15 years, 7 months ago (Jan. 20, 2010)
Published Online	15 years, 7 months ago (Jan. 20, 2010)
Published Print	15 years, 5 months ago (March 1, 2010)

Funders 0

None

BibTeX

@article{Lardelli_2010, title={Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing}, volume={16}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.2030510}, DOI={10.1261/rna.2030510}, number={3}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={Lardelli, Rea M. and Thompson, James X. and Yates, John R. and Stevens, Scott W.}, year={2010}, month=jan, pages={516–528} }

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