DOI: 10.1261/rna.144606. Sequence-specific RNA binding mediated by the RNase PH domain of components of the exosome

Sequence-specific RNA binding mediated by the RNase PH domain of components of the exosome

10.1261/rna.144606

Crossref journal-article

Cold Spring Harbor Laboratory

RNA (246)

Abstract

We have previously demonstrated that PM-Scl-75, a component of the human exosome complex involved in RNA maturation and mRNA decay, can specifically interact with RNAs containing an AU-rich instability element. Through the analysis of a series of deletion mutants, we have now shown that a 266 amino acid fragment representing the RNase PH domain is responsible for the sequence-specific binding to AU-rich elements. Furthermore, we found that the RNase PH domains from two other exosomal components, OIP2 and RRP41, as well as from Escherichia coli polynucleotide phosphorylase, are all capable of specifically interacting with RNAs containing an AU-rich element with similar affinities. Finally, we demonstrate that the interaction of the RNase PH domain of PM-Scl-75 is readily competed by poly(U), but only inefficiently using other homopolymeric RNAs. These data demonstrate that RNase PH domains in general have an affinity for U- and AU-rich sequences, and broaden the potential role in RNA biology of proteins containing these domains.

Bibliography

Anderson, J. R., Mukherjee, D., Muthukumaraswamy, K., Moraes, K. C. M., Wilusz, C. J., & Wilusz, J. (2006). Sequence-specific RNA binding mediated by the RNase PHÂ domain of components of the exosome. RNA, 12(10), 1810â1816.

Authors 6

John R. Anderson (first)
Devi Mukherjee (additional)
Karthika Muthukumaraswamy (additional)
Karen C.M. Moraes (additional)
Carol J. Wilusz (additional)
Jeffrey Wilusz (additional)

References 27 Referenced 29

10.1093/nar/24.20.4063
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10.1016/S0962-8924(01)02225-5
10.1016/j.molcel.2005.10.018
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10.1016/S0968-0004(00)89102-1
de Hoon, M.J. Makita, Y. Nakai, K. Miyano, S. (2005) Prediction of transcriptional terminators in Bacillus subtilis and related species. PLoS Comput. Biol. 1, e25. (10.1371/journal.pcbi.0010025)
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{'key': '2021112105411942000_12.10.1810.19', 'first-page': '59', 'article-title': 'The 3′ end formation in small RNAs', 'volume': '10', 'author': 'Perumal,', 'year': '2002', 'journal-title': 'Gene Expr.'} / Gene Expr. / The 3′ end formation in small RNAs by Perumal, (2002)
10.1093/nar/22.12.2334
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10.1093/nar/29.5.1017

Dates

Type	When
Created	19 years ago (Aug. 15, 2006, 10:48 p.m.)
Deposited	3 years, 9 months ago (Nov. 21, 2021, 8:47 a.m.)
Indexed	1 year, 1 month ago (July 19, 2024, 2:37 a.m.)
Issued	19 years ago (Aug. 15, 2006)
Published	19 years ago (Aug. 15, 2006)
Published Online	19 years ago (Aug. 15, 2006)
Published Print	18 years, 11 months ago (Oct. 1, 2006)

Funders 0

None

BibTeX

@article{Anderson_2006, title={Sequence-specific RNA binding mediated by the RNase PH domain of components of the exosome}, volume={12}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.144606}, DOI={10.1261/rna.144606}, number={10}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={Anderson, John R. and Mukherjee, Devi and Muthukumaraswamy, Karthika and Moraes, Karen C.M. and Wilusz, Carol J. and Wilusz, Jeffrey}, year={2006}, month=aug, pages={1810–1816} }

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