DOI: 10.1261/rna.1313609. Actin filament labels for localizing protein components in large complexes viewed by electron microscopy

Actin filament labels for localizing protein components in large complexes viewed by electron microscopy

10.1261/rna.1313609

Crossref journal-article

Cold Spring Harbor Laboratory

RNA (246)

Abstract

Localizing specific components in three-dimensional reconstructions of protein complexes visualized in an electron microscope increases the scientific value of those structures. Subunits are often identified within the complex by labeling; however, unless the label produces directly visible features, it must be detected by computational comparison with unlabeled complex. To bypass this step, we generated a cloneable tag from the actin-nucleating protein Spire that produces a directly visible “pointer” to the subunit after actin polymerization. We have used this new label to identify the intron of the C complex spliceosome to its small domain by fusing the 10 kDa Spire moiety to the affinity label that binds recombinant stem loops in the pre-mRNA substrate and assembling an actin filament on the particle.

Bibliography

Stroupe, M. E., Xu, C., Goode, B. L., & Grigorieff, N. (2008). Actin filament labels for localizing protein components in large complexes viewed by electron microscopy. RNA, 15(2), 244â248.

Authors 4

M. Elizabeth Stroupe (first)
Chen Xu (additional)
Bruce L. Goode (additional)
Nikolaus Grigorieff (additional)

References 17 Referenced 13

10.1038/nsmb.1378
10.1006/jsbi.1996.0030
10.1017/S1355838202021088
10.1016/S1097-2765(03)00270-3
10.1038/nsmb728
10.1016/S0896-6273(04)00050-9
10.1016/j.jsb.2005.07.007
10.1074/jbc.M503094200
10.1083/jcb.103.6.2747
10.1146/annurev.biophys.35.040405.101936
10.1006/jmbi.2001.4852
10.1038/nature03241
10.1016/j.molcel.2006.08.021
10.1006/jsbi.1998.4073
{'key': '2021112105482280000_15.2.244.15', 'first-page': '4866', 'article-title': 'The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin–troponin complex with actin and the proteolytic fragments of myosin', 'volume': '246', 'author': 'Spudich,', 'year': '1971', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin–troponin complex with actin and the proteolytic fragments of myosin by Spudich, (1971)
10.1016/j.jmb.2006.05.049
10.1016/S0022-2836(03)00899-4

Dates

Type	When
Created	16 years, 8 months ago (Dec. 17, 2008, 9:43 p.m.)
Deposited	3 years, 9 months ago (Nov. 21, 2021, 8:56 a.m.)
Indexed	1 year, 11 months ago (Sept. 15, 2023, 4:03 a.m.)
Issued	16 years, 8 months ago (Dec. 17, 2008)
Published	16 years, 8 months ago (Dec. 17, 2008)
Published Online	16 years, 8 months ago (Dec. 17, 2008)
Published Print	16 years, 6 months ago (Feb. 1, 2009)

Funders 0

None

BibTeX

@article{Stroupe_2008, title={Actin filament labels for localizing protein components in large complexes viewed by electron microscopy}, volume={15}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.1313609}, DOI={10.1261/rna.1313609}, number={2}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={Stroupe, M. Elizabeth and Xu, Chen and Goode, Bruce L. and Grigorieff, Nikolaus}, year={2008}, month=dec, pages={244–248} }

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