DOI: 10.1261/rna.044917.114. The SBP2 protein central to selenoprotein synthesis contacts the human ribosome at expansion segment 7L of the 28S rRNA

The SBP2 protein central to selenoprotein synthesis contacts the human ribosome at expansion segment 7L of the 28S rRNA

10.1261/rna.044917.114

Crossref journal-article

Cold Spring Harbor Laboratory

RNA (246)

Abstract

SBP2 is a pivotal protein component in selenoprotein synthesis. It binds the SECIS stem–loop in the 3′ UTR of selenoprotein mRNA and interacts with both the specialized translation elongation factor and the ribosome at the 60S subunit. In this work, our goal was to identify the binding partners of SBP2 on the ribosome. Cross-linking experiments with bifunctional reagents demonstrated that the SBP2-binding site on the human ribosome is mainly formed by the 28S rRNA. Direct hydroxyl radical probing of the entire 28S rRNA revealed that SBP2 bound to 80S ribosomes or 60S subunits protects helix ES7L-E in expansion segment 7 of the 28S rRNA. Diepoxybutane cross-linking confirmed the interaction of SBP2 with helix ES7L-E. Additionally, binding of SBP2 to the ribosome led to increased reactivity toward chemical probes of a few bases in ES7L-E and in the universally conserved helix H89, indicative of conformational changes in the 28S rRNA in response to SBP2 binding. This study revealed for the first time that SBP2 makes direct contacts with a discrete region of the human 28S rRNA.

Bibliography

Kossinova, O., Malygin, A., Krol, A., & Karpova, G. (2014). The SBP2 protein central to selenoprotein synthesis contacts the human ribosome at expansion segment 7L of the 28S rRNA. RNA, 20(7), 1046â1056.

Authors 4

Olga Kossinova (first)
Alexey Malygin (additional)
Alain Krol (additional)
Galina Karpova (additional)

References 42 Referenced 25

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Dates

Type	When
Created	11 years, 3 months ago (May 22, 2014, 1:26 a.m.)
Deposited	3 years, 9 months ago (Nov. 21, 2021, 9:13 a.m.)
Indexed	2 months, 4 weeks ago (May 28, 2025, 12:13 a.m.)
Issued	11 years, 3 months ago (May 21, 2014)
Published	11 years, 3 months ago (May 21, 2014)
Published Online	11 years, 3 months ago (May 21, 2014)
Published Print	11 years, 1 month ago (July 1, 2014)

Funders 0

None

BibTeX

@article{Kossinova_2014, title={The SBP2 protein central to selenoprotein synthesis contacts the human ribosome at expansion segment 7L of the 28S rRNA}, volume={20}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.044917.114}, DOI={10.1261/rna.044917.114}, number={7}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={Kossinova, Olga and Malygin, Alexey and Krol, Alain and Karpova, Galina}, year={2014}, month=may, pages={1046–1056} }

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