DOI: 10.1246/cl.1980.773. MODIFICATION OF E. COLI ASPARAGINASE WITH 2,4-BIS(<i>O</i>-METHOXYPOLYETHYLENE GLYCOL)-6-CHLORO-<i>S</i>-TRIAZINE(ACTIVATED PEG2); DISAPPEARANCE OF BINDING ABILITY TOWARDS ANTI-SERUM AND RETENTION OF ENZYMIC ACTIVITY

MODIFICATION OF E. COLI ASPARAGINASE WITH 2,4-BIS(O-METHOXYPOLYETHYLENE GLYCOL)-6-CHLORO-S-TRIAZINE(ACTIVATED PEG2); DISAPPEARANCE OF BINDING ABILITY TOWARDS ANTI-SERUM AND RETENTION OF ENZYMIC ACTIVITY

10.1246/cl.1980.773

Crossref journal-article

Oxford University Press (OUP)

Chemistry Letters (286)

Abstract

Abstract Asparaginase from E. coli was modified with activated PEG2 which was synthesized from monomethoxypolyethylene glycol and cyanuric chloride. The PEG2-modified asparaginase had no binding ability towards anti-asparaginase serum but retained 11% of the enzymic activity(GOT method) of the native asparaginase.

Bibliography

Matsushima, A., Nishimura, H., Ashihara, Y., Yokota, Y., & Inada, Y. (1980). MODIFICATION OF E. COLI ASPARAGINASE WITH 2,4-BIS(O-METHOXYPOLYETHYLENE GLYCOL)-6-CHLORO-S-TRIAZINE(ACTIVATED PEG2); DISAPPEARANCE OF BINDING ABILITY TOWARDS ANTI-SERUM AND RETENTION OF ENZYMIC ACTIVITY. Chemistry Letters, 9(7), 773â776.

Authors 5

Ayako Matsushima (first)
Hiroyuki Nishimura (additional)
Yoshihiro Ashihara (additional)
Yoshihiro Yokota (additional)
Yuji Inada (additional)

References 13 Referenced 71

10.1021/bi00571a022
{'key': '2024011902071047100_r2'}
{'key': '2024011902071047100_r3'}
10.1016/0091-6749(79)90139-8
{'key': '2024011902071047100_r5'}
10.1016/0006-291X(78)91002-1
{'key': '2024011902071047100_r7'}
10.1016/0003-2697(66)90275-2
{'key': '2024011902071047100_r9'}
10.1016/0003-2697(74)90284-X
{'key': '2024011902071047100_r11'}
10.1016/0019-2791(75)90115-9
{'key': '2024011902071047100_r13'}

Dates

Type	When
Created	19 years, 1 month ago (July 24, 2006, 10:12 p.m.)
Deposited	1 year, 7 months ago (Jan. 18, 2024, 9:07 p.m.)
Indexed	2 days, 12 hours ago (Sept. 4, 2025, 10:09 a.m.)
Issued	45 years, 2 months ago (July 1, 1980)
Published	45 years, 2 months ago (July 1, 1980)
Published Online	19 years, 5 months ago (March 27, 2006)
Published Print	45 years, 2 months ago (July 5, 1980)

Funders 0

None

BibTeX

@article{Matsushima_1980, title={MODIFICATION OF E. COLI ASPARAGINASE WITH 2,4-BIS(O-METHOXYPOLYETHYLENE GLYCOL)-6-CHLORO-S-TRIAZINE(ACTIVATED PEG2); DISAPPEARANCE OF BINDING ABILITY TOWARDS ANTI-SERUM AND RETENTION OF ENZYMIC ACTIVITY}, volume={9}, ISSN={1348-0715}, url={http://dx.doi.org/10.1246/cl.1980.773}, DOI={10.1246/cl.1980.773}, number={7}, journal={Chemistry Letters}, publisher={Oxford University Press (OUP)}, author={Matsushima, Ayako and Nishimura, Hiroyuki and Ashihara, Yoshihiro and Yokota, Yoshihiro and Inada, Yuji}, year={1980}, month=jul, pages={773–776} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T14:09:16Z",
    "timestamp": 1756994956867
  },
  "reference-count": 13,
  "publisher": "Oxford University Press (OUP)",
  "issue": "7",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1980,
            7,
            1
          ]
        ],
        "date-time": "1980-07-01T00:00:00Z",
        "timestamp": 331257600000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "https://academic.oup.com/pages/standard-publication-reuse-rights"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1980,
        7,
        5
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Asparaginase from E. coli was modified with activated PEG2 which was synthesized from monomethoxypolyethylene glycol and cyanuric chloride. The PEG2-modified asparaginase had no binding ability towards anti-asparaginase serum but retained 11% of the enzymic activity(GOT method) of the native asparaginase.</jats:p>",
  "DOI": "10.1246/cl.1980.773",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        7,
        25
      ]
    ],
    "date-time": "2006-07-25T02:12:34Z",
    "timestamp": 1153793554000
  },
  "page": "773-776",
  "source": "Crossref",
  "is-referenced-by-count": 71,
  "title": "MODIFICATION OF E. COLI ASPARAGINASE WITH 2,4-BIS(<i>O</i>-METHOXYPOLYETHYLENE GLYCOL)-6-CHLORO-<i>S</i>-TRIAZINE(ACTIVATED PEG2); DISAPPEARANCE OF BINDING ABILITY TOWARDS ANTI-SERUM AND RETENTION OF ENZYMIC ACTIVITY",
  "prefix": "10.1093",
  "volume": "9",
  "author": [
    {
      "given": "Ayako",
      "family": "Matsushima",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Laboratory of Biological Chemistry, Tokyo Institute of Technology"
        }
      ]
    },
    {
      "given": "Hiroyuki",
      "family": "Nishimura",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory of Biological Chemistry, Tokyo Institute of Technology"
        }
      ]
    },
    {
      "given": "Yoshihiro",
      "family": "Ashihara",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory of Biological Chemistry, Tokyo Institute of Technology"
        }
      ]
    },
    {
      "given": "Yoshihiro",
      "family": "Yokota",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory of Biological Chemistry, Tokyo Institute of Technology"
        }
      ]
    },
    {
      "given": "Yuji",
      "family": "Inada",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory of Biological Chemistry, Tokyo Institute of Technology"
        }
      ]
    }
  ],
  "member": "286",
  "published-online": {
    "date-parts": [
      [
        2006,
        3,
        27
      ]
    ]
  },
  "reference": [
    {
      "key": "2024011902071047100_r1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00571a022"
    },
    {
      "key": "2024011902071047100_r2"
    },
    {
      "key": "2024011902071047100_r3"
    },
    {
      "key": "2024011902071047100_r4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0091-6749(79)90139-8"
    },
    {
      "key": "2024011902071047100_r5"
    },
    {
      "key": "2024011902071047100_r6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(78)91002-1"
    },
    {
      "key": "2024011902071047100_r7"
    },
    {
      "key": "2024011902071047100_r8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(66)90275-2"
    },
    {
      "key": "2024011902071047100_r9"
    },
    {
      "key": "2024011902071047100_r10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(74)90284-X"
    },
    {
      "key": "2024011902071047100_r11"
    },
    {
      "key": "2024011902071047100_r12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0019-2791(75)90115-9"
    },
    {
      "key": "2024011902071047100_r13"
    }
  ],
  "container-title": "Chemistry Letters",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://academic.oup.com/chemlett/article-pdf/9/7/773/56162864/cl.1980.773.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://academic.oup.com/chemlett/article-pdf/9/7/773/56162864/cl.1980.773.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        19
      ]
    ],
    "date-time": "2024-01-19T02:07:22Z",
    "timestamp": 1705630042000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://academic.oup.com/chemlett/article/9/7/773/7412702"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1980,
        7
      ]
    ]
  },
  "references-count": 13,
  "journal-issue": {
    "issue": "7",
    "published-online": {
      "date-parts": [
        [
          2006,
          3,
          27
        ]
      ]
    },
    "published-print": {
      "date-parts": [
        [
          1980,
          7,
          5
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1246/cl.1980.773",
  "relation": {},
  "ISSN": [
    "0366-7022",
    "1348-0715"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        1980,
        7
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        1980,
        7
      ]
    ]
  }
}