DOI: 10.1242/jeb.205.13.1935. Analysis of Ca2+ uptake into the smooth endoplasmic reticulum of permeabilised sternal epithelial cells during the moulting cycle of the terrestrial isopod<i>Porcellio scaber</i>

Analysis of Ca2+ uptake into the smooth endoplasmic reticulum of permeabilised sternal epithelial cells during the moulting cycle of the terrestrial isopodPorcellio scaber

10.1242/jeb.205.13.1935

Crossref journal-article

The Company of Biologists

Journal of Experimental Biology (237)

Abstract

SUMMARYIn terrestrial isopods, large amounts of Ca2+ are transported across anterior sternal epithelial cells during moult-related deposition and resorption of CaCO3 deposits. Because of its toxicity and function as a second messenger, resting cytosolic Ca2+ levels must be maintained below critical concentrations during epithelial Ca2+transport, raising the possibility that organelles play a role during Ca2+ transit. We therefore studied the uptake of Ca2+into Ca2+-sequestering organelles by monitoring the formation of birefringent calcium oxalate crystals in permeabilised anterior and posterior sternal epithelium cells of Porcellio scaber during Ca2+-transporting and non-transporting stages of the moulting cycle using polarised-light microscopy. The results indicate ATP-dependent uptake of Ca2+ into organelles. Half-maximal crystal growth at a Ca2+ activity, aCa, of 0.4 μmol l-1 and blockade by cyclopiazonic acid suggest Ca2+uptake into the smooth endoplasmic reticulum by the smooth endoplasmic reticulum Ca2+-ATPase. Analytical electron microscopical techniques support this interpretation by revealing the accumulation of Ca2+-containing crystals in smooth membranous intracellular compartments. A comparison of different moulting stages demonstrated a virtual lack of crystal formation in the early premoult stage and a significant fivefold increase between mid premoult and the Ca2+-transporting stages of late premoult and intramoult. These results suggest a contribution of the smooth endoplasmic reticulum as a transient Ca2+ store during intracellular Ca2+ transit.

Bibliography

Hagedorn, M., & Ziegler, A. (2002). Analysis of Ca2+ uptake into the smooth endoplasmic reticulum of permeabilised sternal epithelial cells during the moulting cycle of the terrestrial isopodPorcellio scaber. Journal of Experimental Biology, 205(13), 1935â1942.

Authors 2

Monica Hagedorn (first)
Andreas Ziegler (additional)

References 38 Referenced 26

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 12:52 a.m.)
Deposited	1 year, 9 months ago (Nov. 2, 2023, 1:55 p.m.)
Indexed	1 year, 3 months ago (May 9, 2024, 12:38 a.m.)
Issued	23 years, 1 month ago (July 1, 2002)
Published	23 years, 1 month ago (July 1, 2002)
Published Print	23 years, 1 month ago (July 1, 2002)

Funders 0

None

BibTeX

@article{Hagedorn_2002, title={Analysis of Ca2+ uptake into the smooth endoplasmic reticulum of permeabilised sternal epithelial cells during the moulting cycle of the terrestrial isopodPorcellio scaber}, volume={205}, ISSN={0022-0949}, url={http://dx.doi.org/10.1242/jeb.205.13.1935}, DOI={10.1242/jeb.205.13.1935}, number={13}, journal={Journal of Experimental Biology}, publisher={The Company of Biologists}, author={Hagedorn, Monica and Ziegler, Andreas}, year={2002}, month=jul, pages={1935–1942} }

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  "abstract": "<jats:title>SUMMARY</jats:title><jats:p>In terrestrial isopods, large amounts of Ca2+ are transported across anterior sternal epithelial cells during moult-related deposition and resorption of CaCO3 deposits. Because of its toxicity and function as a second messenger, resting cytosolic Ca2+ levels must be maintained below critical concentrations during epithelial Ca2+transport, raising the possibility that organelles play a role during Ca2+ transit. We therefore studied the uptake of Ca2+into Ca2+-sequestering organelles by monitoring the formation of birefringent calcium oxalate crystals in permeabilised anterior and posterior sternal epithelium cells of Porcellio scaber during Ca2+-transporting and non-transporting stages of the moulting cycle using polarised-light microscopy. The results indicate ATP-dependent uptake of Ca2+ into organelles. Half-maximal crystal growth at a Ca2+ activity, aCa, of 0.4 \u03bcmol l-1 and blockade by cyclopiazonic acid suggest Ca2+uptake into the smooth endoplasmic reticulum by the smooth endoplasmic reticulum Ca2+-ATPase. Analytical electron microscopical techniques support this interpretation by revealing the accumulation of Ca2+-containing crystals in smooth membranous intracellular compartments. A comparison of different moulting stages demonstrated a virtual lack of crystal formation in the early premoult stage and a significant fivefold increase between mid premoult and the Ca2+-transporting stages of late premoult and intramoult. These results suggest a contribution of the smooth endoplasmic reticulum as a transient Ca2+ store during intracellular Ca2+ transit.</jats:p>",
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