Characteristic features and ligand specificity of the two olfactory receptor classes fromXenopus laevis
10.1242/jeb.204.17.2987
Crossref journal-article
The Company of Biologists
Journal of Experimental Biology (237)
Abstract

SUMMARYAmphibia have two classes of olfactory receptors (ORs), class I (fish-like receptors) and class II (mammalian-like receptors). These two receptor classes correspond to the two classes identified in other vertebrates, and amphibians thus provide a unique opportunity to compare olfactory receptors of both classes in one animal species, without the constraints of evolutionary distance between different vertebrate orders, such as fish and mammals. We therefore identified the complete open reading frames of class I and class II ORs in Xenopus laevis. In addition to allowing a representative comparison of the deduced amino acid sequences between both receptor classes, we were also able to perform differential functional analysis. These studies revealed distinct class-specific motifs, particularly in the extracellular loops 2 and 3, which might be of importance for the interaction with odorants, as well as in the intracellular loops 2 and 3, which might be responsible for interactions with specific G-proteins. The results of functional expression studies in Xenopus oocytes, comparing distinct receptor types, support the idea that class I receptors are activated by water-soluble odorants, whereas class II receptors are activated by volatile compounds.

Bibliography

Mezler, M., Fleischer, J., & Breer, H. (2001). Characteristic features and ligand specificity of the two olfactory receptor classes fromXenopus laevis. Journal of Experimental Biology, 204(17), 2987–2997.

Authors 3
  1. Mario Mezler (first)
  2. Jörg Fleischer (additional)
  3. Heinz Breer (additional)
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Dates
Type When
Created 4 years, 4 months ago (April 23, 2021, 7:43 p.m.)
Deposited 1 year, 10 months ago (Nov. 2, 2023, 1:19 p.m.)
Indexed 1 year, 9 months ago (Nov. 21, 2023, 12:31 a.m.)
Issued 24 years ago (Sept. 1, 2001)
Published 24 years ago (Sept. 1, 2001)
Published Print 24 years ago (Sept. 1, 2001)
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@article{Mezler_2001, title={Characteristic features and ligand specificity of the two olfactory receptor classes fromXenopus laevis}, volume={204}, ISSN={0022-0949}, url={http://dx.doi.org/10.1242/jeb.204.17.2987}, DOI={10.1242/jeb.204.17.2987}, number={17}, journal={Journal of Experimental Biology}, publisher={The Company of Biologists}, author={Mezler, Mario and Fleischer, Jörg and Breer, Heinz}, year={2001}, month=sep, pages={2987–2997} }