DOI: 10.1242/jeb.199.5.1147. A Novel Subunit of Vacuolar H+-ATPase Related to The <i>b</i> Subunit of F-ATPases

A Novel Subunit of Vacuolar H+-ATPase Related to The b Subunit of F-ATPases

10.1242/jeb.199.5.1147

Crossref journal-article

The Company of Biologists

Journal of Experimental Biology (237)

Abstract

ABSTRACT The subunit structure of the vacuolar H+-ATPase (V-ATPase) membrane sector is not entirely known. The proteolipid is the only subunit that has been implicated in the mechanism of energy transfer in the enzyme. We have identified a protein (M16) that co-purifies with the V-ATPase complex from bovine chromaffin granules. Information obtained from the amino acid sequence of a proteolytic fragment of M16 was used to clone a bovine adrenal cDNA encoding this protein. The cDNA encodes a hydrophilic protein of 118 amino acid residues with a calculated molecular mass of 13 682 Da. Amino acid sequence analysis revealed that M16 exhibits a significant homology to subunit b of F-ATPases. M16 is smaller than subunit b and contains no apparent transmembrane segment in its N terminus. The remainder of subunit b is related to M16 not only by its amino acid sequence but also in its predicted structure of helix–turn–helix. The structural and evolutionary implications of these findings are discussed.

Bibliography

Supekova, L., Sbia, M., Supek, F., Ma, Y., & Nelson, N. (1996). A Novel Subunit of Vacuolar H+-ATPase Related to The b Subunit of F-ATPases. Journal of Experimental Biology, 199(5), 1147â1156.

Authors 5

Lubica Supekova (first)
Mohammed Sbia (additional)
Frantisek Supek (additional)
Yuemei Ma (additional)
Nathan Nelson (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 12:03 a.m.)
Deposited	1 year, 5 months ago (March 17, 2024, 5:32 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 6, 2025, 8:27 a.m.)
Issued	29 years, 3 months ago (May 1, 1996)
Published	29 years, 3 months ago (May 1, 1996)
Published Online	29 years, 3 months ago (May 1, 1996)
Published Print	29 years, 3 months ago (May 1, 1996)

Funders 0

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BibTeX

@article{Supekova_1996, title={A Novel Subunit of Vacuolar H+-ATPase Related to The b Subunit of F-ATPases}, volume={199}, ISSN={1477-9145}, url={http://dx.doi.org/10.1242/jeb.199.5.1147}, DOI={10.1242/jeb.199.5.1147}, number={5}, journal={Journal of Experimental Biology}, publisher={The Company of Biologists}, author={Supekova, Lubica and Sbia, Mohammed and Supek, Frantisek and Ma, Yuemei and Nelson, Nathan}, year={1996}, month=may, pages={1147–1156} }

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