DOI: 10.1242/jcs.97.1.109. Biochemical and immunochemical analysis of the arrangement of connexin43 in rat heart gap junction membranes

Biochemical and immunochemical analysis of the arrangement of connexin43 in rat heart gap junction membranes

10.1242/jcs.97.1.109

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT A 43 × 10 3 M r protein (designated connexin 43 or Cx43) is a major constituent of heart gap junctions. The understanding of its arrangement in junctional membranes has been extended by means of site-directed antibodies raised against synthetic peptides of Cx43. These represent part of the first extracellular loop (EL-46), the cytoplasmic loop (CL-100), the second extracellular loop (EL-186) and carboxy-terminal sequences (CT-237 and CT-360). All of the antibodies raised reacted with their respective peptides and the Cx43 protein on Western blots. By immunoelectron microscopy two of the antibodies (CL-100 and CT-360) were shown to label the cytoplasmic surface of isolated gap junction membranes. Immunofluor-escent labeling at locations of neonatal cardiac myocyte-myocyte apposition required an alkali/urea treatment when the EL-46 and EL-186 antibodies were used. Immunoblot analysis of endoproteinase Lys-C-digested gap junctions revealed that the Cx43 protein passed through the lipid bilayer four times. Alkaline phosphatase digestion of isolated junctions was used to show that the CT-360 antibody recognized many phosphorylated forms of Cx43. Our results unequivocally confirm models of the organization of Cx43 that were based on a more limited set of data and a priori considerations of the sequence.

Bibliography

Laird, D. W., & Revel, J.-P. (1990). Biochemical and immunochemical analysis of the arrangement of connexin43 in rat heart gap junction membranes. Journal of Cell Science, 97(1), 109â117.

Authors 2

D. W. Laird (first)
J.-P. Revel (additional)

References 35 Referenced 127

{'key': '2023100700222296700_JOCES_97_1_109C1', 'first-page': '167', 'article-title': 'The connexins, a family of related gap junction proteins', 'volume-title': 'In Modern Cell Biology: Gap Junctions', 'author': 'Beyer', 'year': '1988'} / In Modern Cell Biology: Gap Junctions / The connexins, a family of related gap junction proteins by Beyer (1988)
10.1083/jcb.108.2.595 / J. Cell Biol / Antisera directed against connexin43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues by Bbybr (1989)
10.1083/jcb.105.6.2621 / J. Cell Biol / Connexin43: A protein from rat heart homologous to a gap junction protein from liver by Beyer (1987)
10.1016/0022-2828(82)90198-5 / J. molec. cell. Cardiol / Electrical and contractile consequences on Na + or Ca 4+ gradient reduction in cultured heart cells by Burt (1982)
10.1073/pnas.85.10.3431 / Proc. natn. Acad. Sci. U.SA / Single-channel events and gating behavior of the cardiac gap junction channel by Bubt (1988)
{'key': '2023100700222296700_JOCES_97_1_109C6', 'first-page': '299', 'article-title': 'Intercellular coupling of embryonic heart cells', 'volume-title': 'In Perspectives in Cardiovascular Research', 'author': 'De Haan', 'year': '1981'} / In Perspectives in Cardiovascular Research / Intercellular coupling of embryonic heart cells by De Haan (1981)
10.1007/BF01870924 / J. Membr. Biol / Immunological characterization of rat cardiac gap junctions. Presence of common antigenic determinants in heart of other vertebrate species and in various organs by Dupont (1988)
10.1016/0040-8166(85)90001-1 / Tissue & Cell / Intercalated discs of mammalian heart: A review of structure and function by Forbes (1985)
{'key': '2023100700222296700_JOCES_97_1_109C9', 'first-page': '395', 'article-title': 'Gap junctional contact between cells', 'volume': '125', 'author': 'Gilula', 'year': '1987', 'journal-title': 'CIBA Fdn Symp'} / CIBA Fdn Symp / Gap junctional contact between cells by Gilula (1987)
10.1083/jcb.107.5.1817 / J. Cell Biol / Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions by Goodenough (1988)
10.1016/0014-4827(78)90366-X / Expl Cell Res / Acquisition of synchronous beating between embryonic heart cell aggregates and layers by Griepp (1978)
{'key': '2023100700222296700_JOCES_97_1_109C12', 'volume-title': 'Antibodies: A Laboratory Manual', 'author': 'Harlow', 'year': '1988'} / Antibodies: A Laboratory Manual by Harlow (1988)
10.1016/S0021-9258(18)37396-4 / J. biol. Chem / Topology of the Mr 27,000 liver gap junction protein by Hertzberc (1988)
10.1073/pnas.82.8.2412 / Proc. natn. Acad. Sci. U.S.A / Reduction of gap junctional conductance by microinjection of antibodies against the 27-kDa liver gap junction polypeptide by Hertzberg (1985)
10.1038/227680a0 / Nature / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli (1970)
10.1007/BF01869201 / J. Membr. Biol / Detergent sensitivity and splitting of isolated liver gap junctions by Manjunath (1984)
{'key': '2023100700222296700_JOCES_97_1_109C17', 'first-page': '865', 'article-title': 'Cytoplasmic surface and intramembrane components of rat heart gap junction proteins', 'volume': '246', 'author': 'Manjunath', 'year': '1984', 'journal-title': 'Am. J. Physiol'} / Am. J. Physiol / Cytoplasmic surface and intramembrane components of rat heart gap junction proteins by Manjunath (1984)
10.1007/BF01871268 / J. Membr. Biol / Proteolysis of cardiac gap junctions during their isolation from rat hearts by Manjunath (1985)
10.1016/0006-291X(87)90475-X / Biochem biophys. Res. Commun / The cardiac gap junction protein (Mr 47,000) has a tissue specific cytoplasmic domain of Mr 17,000 at its carboxy terminus by Manjunath (1987)
10.1007/BF01869685 / J. Membr. Biol / Rat heart gap junctions as disulphide bonded connexon multimers: Their depolymerization and solubilization in deoxycholate by Manjunath (1986)
10.1016/0014-4827(66)90427-7 / Expl Cell Res / Pacemaker activity and mitosis in cultures of newborn rat heart ventricle cells by Mark (1966)
10.1002/j.1460-2075.1988.tb03159.x / EMBO J / Topology of the 32-kd liver gap junction protein determined by site-directed antibody localizations by Milks (1988)
10.1016/0896-6273(88)90109-2 / Neuron / Sequences of autophosphorylation sites in neuronal type II CaM kinase that control Ca 2+-independent activity by Miller (1988)
{'key': '2023100700222296700_JOCES_97_1_109C24', 'first-page': '207', 'article-title': 'Multiple protein components in a single gap junction: Cloning of a second hepatic gap junction protein (M r 21,000', 'volume-title': 'In Modern Cell Biology: Gap Junctions', 'author': 'Nicholson', 'year': '1988'} / In Modern Cell Biology: Gap Junctions / Multiple protein components in a single gap junction: Cloning of a second hepatic gap junction protein (M r 21,000 by Nicholson (1988)
10.1083/jcb.103.1.123 / J. Cell Biol / Molecule cloning of cDNA for rat liver gap junction protein by Paul (1986)
10.1007/978-1-4684-5050-7_10 / In The Cell Surface in Cancer and Development / Gap junctions in development by Revel (1986)
10.1007/BF00585633 / Eur. J. Physiol / Single channel currents of homo- and heterologous gap junctions between cardiac fibroblasts and myocytes by Rook (1989)
10.1073/pnas.83.8.2473 / Proc. natn. Acad. Sci. U.S.A / cAMP increases junctional conductance and stimulates phosphorylation of the 27-kDa principal gap junction polypeptide by Saez (1986)
10.1161/01.RES.65.1.22 / Circulation Res / Fate of gap junctions in isolated adult mammalian cardiomyocytes by Severs (1989)
{'key': '2023100700222296700_JOCES_97_1_109C30', 'first-page': 'H753', 'article-title': 'Regulation of gap junctional conductance', 'volume': '248', 'author': 'Spray', 'year': '1985', 'journal-title': 'Am. J. Physiol'} / Am. J. Physiol / Regulation of gap junctional conductance by Spray (1985)
10.1016/0092-8674(89)90180-3 / Cell / Formation of gap junctions by expression of connexins in Xenopus oocyte pairs by Swenson (1989)
10.1038/311127a0 / Nature / Antibodies to gap-junctional protein selectively disrupt communication in the early amphibian embryo by Warner (1984)
10.1083/jcb.108.6.2241 / J. Cell Biol / The 43-kD polypeptide of heart gap junctions: Immunolocalization (I), topology (ID and functional domains (III) by Yancey (1989)
10.1083/jcb.109.6.3391 / J. Cell Biol / Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA by Zhang (1989)
10.1016/S0021-9258(18)47632-6 / J. biol. Chem / Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures by Zimmer (1987)

Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 5:22 p.m.)
Deposited	1 year, 10 months ago (Oct. 6, 2023, 8:23 p.m.)
Indexed	3 months, 1 week ago (May 20, 2025, 10:34 a.m.)
Issued	34 years, 11 months ago (Sept. 1, 1990)
Published	34 years, 11 months ago (Sept. 1, 1990)
Published Online	34 years, 11 months ago (Sept. 1, 1990)
Published Print	34 years, 11 months ago (Sept. 1, 1990)

Funders 0

None

BibTeX

@article{Laird_1990, title={Biochemical and immunochemical analysis of the arrangement of connexin43 in rat heart gap junction membranes}, volume={97}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.97.1.109}, DOI={10.1242/jcs.97.1.109}, number={1}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Laird, D. W. and Revel, J.-P.}, year={1990}, month=sep, pages={109–117} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        5,
        20
      ]
    ],
    "date-time": "2025-05-20T14:34:08Z",
    "timestamp": 1747751648317
  },
  "reference-count": 35,
  "publisher": "The Company of Biologists",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1990,
            9,
            1
          ]
        ],
        "date-time": "1990-09-01T00:00:00Z",
        "timestamp": 652147200000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://www.biologists.com/user-licence-1-1/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1990,
        9,
        1
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n               <jats:p>A 43 \u00d7 10 3 M r protein (designated connexin 43 or Cx43) is a major constituent of heart gap junctions. The understanding of its arrangement in junctional membranes has been extended by means of site-directed antibodies raised against synthetic peptides of Cx43. These represent part of the first extracellular loop (EL-46), the cytoplasmic loop (CL-100), the second extracellular loop (EL-186) and carboxy-terminal sequences (CT-237 and CT-360). All of the antibodies raised reacted with their respective peptides and the Cx43 protein on Western blots. By immunoelectron microscopy two of the antibodies (CL-100 and CT-360) were shown to label the cytoplasmic surface of isolated gap junction membranes. Immunofluor-escent labeling at locations of neonatal cardiac myocyte-myocyte apposition required an alkali/urea treatment when the EL-46 and EL-186 antibodies were used. Immunoblot analysis of endoproteinase Lys-C-digested gap junctions revealed that the Cx43 protein passed through the lipid bilayer four times. Alkaline phosphatase digestion of isolated junctions was used to show that the CT-360 antibody recognized many phosphorylated forms of Cx43. Our results unequivocally confirm models of the organization of Cx43 that were based on a more limited set of data and a priori considerations of the sequence.</jats:p>",
  "DOI": "10.1242/jcs.97.1.109",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2021,
        4,
        26
      ]
    ],
    "date-time": "2021-04-26T21:22:21Z",
    "timestamp": 1619472141000
  },
  "page": "109-117",
  "source": "Crossref",
  "is-referenced-by-count": 127,
  "title": "Biochemical and immunochemical analysis of the arrangement of connexin43 in rat heart gap junction membranes",
  "prefix": "10.1242",
  "volume": "97",
  "author": [
    {
      "given": "D. W.",
      "family": "Laird",
      "sequence": "first",
      "affiliation": [
        {
          "name": "California Institute of Technology, Pasadena Division of Biology , , California 91125, USA"
        }
      ]
    },
    {
      "given": "J.-P.",
      "family": "Revel",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "California Institute of Technology, Pasadena Division of Biology , , California 91125, USA"
        }
      ]
    }
  ],
  "member": "237",
  "published-online": {
    "date-parts": [
      [
        1990,
        9,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2023100700222296700_JOCES_97_1_109C1",
      "first-page": "167",
      "article-title": "The connexins, a family of related gap junction proteins",
      "volume-title": "In Modern Cell Biology: Gap Junctions",
      "author": "Beyer",
      "year": "1988"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C2",
      "doi-asserted-by": "publisher",
      "first-page": "595",
      "DOI": "10.1083/jcb.108.2.595",
      "article-title": "Antisera directed against connexin43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues",
      "volume": "108",
      "author": "Bbybr",
      "year": "1989",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C3",
      "doi-asserted-by": "publisher",
      "first-page": "2621",
      "DOI": "10.1083/jcb.105.6.2621",
      "article-title": "Connexin43: A protein from rat heart homologous to a gap junction protein from liver",
      "volume": "105",
      "author": "Beyer",
      "year": "1987",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C4",
      "doi-asserted-by": "publisher",
      "first-page": "99",
      "DOI": "10.1016/0022-2828(82)90198-5",
      "article-title": "Electrical and contractile consequences on Na + or Ca 4+ gradient reduction in cultured heart cells",
      "volume": "14",
      "author": "Burt",
      "year": "1982",
      "journal-title": "J. molec. cell. Cardiol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C5",
      "doi-asserted-by": "publisher",
      "first-page": "3431",
      "DOI": "10.1073/pnas.85.10.3431",
      "article-title": "Single-channel events and gating behavior of the cardiac gap junction channel",
      "volume": "85",
      "author": "Bubt",
      "year": "1988",
      "journal-title": "Proc. natn. Acad. Sci. U.SA"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C6",
      "first-page": "299",
      "article-title": "Intercellular coupling of embryonic heart cells",
      "volume-title": "In Perspectives in Cardiovascular Research",
      "author": "De Haan",
      "year": "1981"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C7",
      "doi-asserted-by": "publisher",
      "first-page": "119",
      "DOI": "10.1007/BF01870924",
      "article-title": "Immunological characterization of rat cardiac gap junctions. Presence of common antigenic determinants in heart of other vertebrate species and in various organs",
      "volume": "104",
      "author": "Dupont",
      "year": "1988",
      "journal-title": "J. Membr. Biol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C8",
      "doi-asserted-by": "publisher",
      "first-page": "605",
      "DOI": "10.1016/0040-8166(85)90001-1",
      "article-title": "Intercalated discs of mammalian heart: A review of structure and function",
      "volume": "17",
      "author": "Forbes",
      "year": "1985",
      "journal-title": "Tissue & Cell"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C9",
      "first-page": "395",
      "article-title": "Gap junctional contact between cells",
      "volume": "125",
      "author": "Gilula",
      "year": "1987",
      "journal-title": "CIBA Fdn Symp"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C10",
      "doi-asserted-by": "publisher",
      "first-page": "1817",
      "DOI": "10.1083/jcb.107.5.1817",
      "article-title": "Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions",
      "volume": "107",
      "author": "Goodenough",
      "year": "1988",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C11",
      "doi-asserted-by": "publisher",
      "first-page": "263",
      "DOI": "10.1016/0014-4827(78)90366-X",
      "article-title": "Acquisition of synchronous beating between embryonic heart cell aggregates and layers",
      "volume": "113",
      "author": "Griepp",
      "year": "1978",
      "journal-title": "Expl Cell Res"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C12",
      "volume-title": "Antibodies: A Laboratory Manual",
      "author": "Harlow",
      "year": "1988"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C13",
      "doi-asserted-by": "publisher",
      "first-page": "19 105",
      "DOI": "10.1016/S0021-9258(18)37396-4",
      "article-title": "Topology of the Mr 27,000 liver gap junction protein",
      "volume": "263",
      "author": "Hertzberc",
      "year": "1988",
      "journal-title": "J. biol. Chem"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C14",
      "doi-asserted-by": "publisher",
      "first-page": "2412",
      "DOI": "10.1073/pnas.82.8.2412",
      "article-title": "Reduction of gap junctional conductance by microinjection of antibodies against the 27-kDa liver gap junction polypeptide",
      "volume": "82",
      "author": "Hertzberg",
      "year": "1985",
      "journal-title": "Proc. natn. Acad. Sci. U.S.A"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C15",
      "doi-asserted-by": "publisher",
      "first-page": "680",
      "DOI": "10.1038/227680a0",
      "article-title": "Cleavage of structural proteins during the assembly of the head of bacteriophage T4",
      "volume": "227",
      "author": "Laemmli",
      "year": "1970",
      "journal-title": "Nature"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C16",
      "doi-asserted-by": "publisher",
      "first-page": "147",
      "DOI": "10.1007/BF01869201",
      "article-title": "Detergent sensitivity and splitting of isolated liver gap junctions",
      "volume": "78",
      "author": "Manjunath",
      "year": "1984",
      "journal-title": "J. Membr. Biol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C17",
      "first-page": "865",
      "article-title": "Cytoplasmic surface and intramembrane components of rat heart gap junction proteins",
      "volume": "246",
      "author": "Manjunath",
      "year": "1984",
      "journal-title": "Am. J. Physiol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C18",
      "doi-asserted-by": "publisher",
      "first-page": "159",
      "DOI": "10.1007/BF01871268",
      "article-title": "Proteolysis of cardiac gap junctions during their isolation from rat hearts",
      "volume": "85",
      "author": "Manjunath",
      "year": "1985",
      "journal-title": "J. Membr. Biol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C19",
      "doi-asserted-by": "publisher",
      "first-page": "228",
      "DOI": "10.1016/0006-291X(87)90475-X",
      "article-title": "The cardiac gap junction protein (Mr 47,000) has a tissue specific cytoplasmic domain of Mr 17,000 at its carboxy terminus",
      "volume": "142",
      "author": "Manjunath",
      "year": "1987",
      "journal-title": "Biochem biophys. Res. Commun"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C20",
      "doi-asserted-by": "publisher",
      "first-page": "43",
      "DOI": "10.1007/BF01869685",
      "article-title": "Rat heart gap junctions as disulphide bonded connexon multimers: Their depolymerization and solubilization in deoxycholate",
      "volume": "90",
      "author": "Manjunath",
      "year": "1986",
      "journal-title": "J. Membr. Biol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C21",
      "doi-asserted-by": "publisher",
      "first-page": "217",
      "DOI": "10.1016/0014-4827(66)90427-7",
      "article-title": "Pacemaker activity and mitosis in cultures of newborn rat heart ventricle cells",
      "volume": "44",
      "author": "Mark",
      "year": "1966",
      "journal-title": "Expl Cell Res"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C22",
      "doi-asserted-by": "publisher",
      "first-page": "2967",
      "DOI": "10.1002/j.1460-2075.1988.tb03159.x",
      "article-title": "Topology of the 32-kd liver gap junction protein determined by site-directed antibody localizations",
      "volume": "7",
      "author": "Milks",
      "year": "1988",
      "journal-title": "EMBO J"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C23",
      "doi-asserted-by": "publisher",
      "first-page": "593",
      "DOI": "10.1016/0896-6273(88)90109-2",
      "article-title": "Sequences of autophosphorylation sites in neuronal type II CaM kinase that control Ca 2+-independent activity",
      "volume": "1",
      "author": "Miller",
      "year": "1988",
      "journal-title": "Neuron"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C24",
      "first-page": "207",
      "article-title": "Multiple protein components in a single gap junction: Cloning of a second hepatic gap junction protein (M r 21,000",
      "volume-title": "In Modern Cell Biology: Gap Junctions",
      "author": "Nicholson",
      "year": "1988"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C25",
      "doi-asserted-by": "publisher",
      "first-page": "123",
      "DOI": "10.1083/jcb.103.1.123",
      "article-title": "Molecule cloning of cDNA for rat liver gap junction protein",
      "volume": "103",
      "author": "Paul",
      "year": "1986",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C26",
      "doi-asserted-by": "crossref",
      "first-page": "189",
      "DOI": "10.1007/978-1-4684-5050-7_10",
      "article-title": "Gap junctions in development",
      "volume-title": "In The Cell Surface in Cancer and Development",
      "author": "Revel",
      "year": "1986"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C27",
      "doi-asserted-by": "publisher",
      "first-page": "95",
      "DOI": "10.1007/BF00585633",
      "article-title": "Single channel currents of homo- and heterologous gap junctions between cardiac fibroblasts and myocytes",
      "volume": "414",
      "author": "Rook",
      "year": "1989",
      "journal-title": "Eur. J. Physiol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C28",
      "doi-asserted-by": "publisher",
      "first-page": "2473",
      "DOI": "10.1073/pnas.83.8.2473",
      "article-title": "cAMP increases junctional conductance and stimulates phosphorylation of the 27-kDa principal gap junction polypeptide",
      "volume": "83",
      "author": "Saez",
      "year": "1986",
      "journal-title": "Proc. natn. Acad. Sci. U.S.A"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C29",
      "doi-asserted-by": "publisher",
      "first-page": "22",
      "DOI": "10.1161/01.RES.65.1.22",
      "article-title": "Fate of gap junctions in isolated adult mammalian cardiomyocytes",
      "volume": "65",
      "author": "Severs",
      "year": "1989",
      "journal-title": "Circulation Res"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C30",
      "first-page": "H753",
      "article-title": "Regulation of gap junctional conductance",
      "volume": "248",
      "author": "Spray",
      "year": "1985",
      "journal-title": "Am. J. Physiol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C31",
      "doi-asserted-by": "publisher",
      "first-page": "145",
      "DOI": "10.1016/0092-8674(89)90180-3",
      "article-title": "Formation of gap junctions by expression of connexins in Xenopus oocyte pairs",
      "volume": "57",
      "author": "Swenson",
      "year": "1989",
      "journal-title": "Cell"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C32",
      "doi-asserted-by": "publisher",
      "first-page": "127",
      "DOI": "10.1038/311127a0",
      "article-title": "Antibodies to gap-junctional protein selectively disrupt communication in the early amphibian embryo",
      "volume": "311",
      "author": "Warner",
      "year": "1984",
      "journal-title": "Nature"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C33",
      "doi-asserted-by": "publisher",
      "first-page": "2241",
      "DOI": "10.1083/jcb.108.6.2241",
      "article-title": "The 43-kD polypeptide of heart gap junctions: Immunolocalization (I), topology (ID and functional domains (III)",
      "volume": "108",
      "author": "Yancey",
      "year": "1989",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C34",
      "doi-asserted-by": "publisher",
      "first-page": "3391",
      "DOI": "10.1083/jcb.109.6.3391",
      "article-title": "Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA",
      "volume": "109",
      "author": "Zhang",
      "year": "1989",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "2023100700222296700_JOCES_97_1_109C35",
      "doi-asserted-by": "publisher",
      "first-page": "7751",
      "DOI": "10.1016/S0021-9258(18)47632-6",
      "article-title": "Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures",
      "volume": "262",
      "author": "Zimmer",
      "year": "1987",
      "journal-title": "J. biol. Chem"
    }
  ],
  "container-title": "Journal of Cell Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.biologists.com/jcs/article-pdf/97/1/109/3202086/joces_97_1_109.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://journals.biologists.com/jcs/article-pdf/97/1/109/3202086/joces_97_1_109.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        7
      ]
    ],
    "date-time": "2023-10-07T00:23:22Z",
    "timestamp": 1696638202000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.biologists.com/jcs/article/97/1/109/60808/Biochemical-and-immunochemical-analysis-of-the"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1990,
        9,
        1
      ]
    ]
  },
  "references-count": 35,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1990,
          9,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1242/jcs.97.1.109",
  "relation": {},
  "ISSN": [
    "0021-9533",
    "1477-9137"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1990,
        9,
        1
      ]
    ]
  }
}