DOI: 10.1242/jcs.96.4.683. Cell cycle regulation of p34 <i>cdc2</i> kinase activity in <i>Physarum polycephalum</i>

Cell cycle regulation of p34 cdc2 kinase activity in Physarum polycephalum

10.1242/jcs.96.4.683

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT The regulation of the mitotic histone H1 kinase activity has been analyzed during the naturally synchronous cell cycle of Physarum polycephalum plasmodia. The universal binding property of the pl3suo1Schizosaccharomyces pombe gene product was used to precipitate and assay the cdc2 histone H1 kinase activity. The kinase activity peaks at the beginning of metaphase and its decline, which requires protein synthesis, appears to be an early event during the metaphase process. Microtubular poisons, temperature shifts and DNA synthesis inhibitors were used to perturb cell cycle regulatory pathways and characterize their effects on cdc2 kinase activation. Our results suggest that the full activation of the mitotic kinase requires at least two successive triggering signals involving microtubular components and DNA synthesis.

Bibliography

Ducommun, B., Tollon, Y., GarÃ©s, M., Beach, D., & Wright, M. (1990). Cell cycle regulation of p34 cdc2 kinase activity in Physarum polycephalum. Journal of Cell Science, 96(4), 683â689.

Authors 5

Bernard Ducommun (first)
Yvette Tollon (additional)
Michéle Garés (additional)
David Beach (additional)
Michel Wright (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 5:23 p.m.)
Deposited	1 year, 2 months ago (June 28, 2024, 9:26 p.m.)
Indexed	6 days, 23 hours ago (Aug. 23, 2025, 1:22 a.m.)
Issued	35 years ago (Aug. 1, 1990)
Published	35 years ago (Aug. 1, 1990)
Published Online	35 years ago (Aug. 1, 1990)
Published Print	35 years ago (Aug. 1, 1990)

Funders 0

None

BibTeX

@article{Ducommun_1990, title={Cell cycle regulation of p34 cdc2 kinase activity in Physarum polycephalum}, volume={96}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.96.4.683}, DOI={10.1242/jcs.96.4.683}, number={4}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Ducommun, Bernard and Tollon, Yvette and Garés, Michéle and Beach, David and Wright, Michel}, year={1990}, month=aug, pages={683–689} }

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