DOI: 10.1242/jcs.96.2.283. Spectrin, actin and the structure of the cortical lattice in mammalian cochlear outer hair cells

Spectrin, actin and the structure of the cortical lattice in mammalian cochlear outer hair cells

10.1242/jcs.96.2.283

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT Mammalian cochlear outer hair cells generate high-frequency forces in response to electrical stimulation. Force generation occurs in the lateral cortex of the cell, which includes the plasma membrane, a two-dimensional ‘cortical lattice’ of filamentous protein, and a multi-layered membrane system, the lateral cistemae. The cortical lattice is composed of relatively long filaments, 6.7 nm in diameter, which are wound circumferentially about the cell. These filaments are spaced about 42 nm apart and are cross-linked by a second type of filament 3.2 nm in diameter approximately aligned with the longitudinal axis of the cell. The cortical lattice is the only cortical structure that remains after the cell is fully extracted in the detergent Triton X-100 and high-salt solution. It retains the original cylindrical shape of the cell and is reversibly deformable. Antibodies raised against chicken gizzard actin, human blood spectrin and pig brain spectrin all react positively with the extracted lattice viewed using immunofluorescence. Three protein subunits identified in the organ of Corti have approximate molecular weights of 220, 235 and 240K (K= 103Mr) and react with the spectrin antibodies. A structural model of the lattice is proposed in which the circumferential filaments are composed of actin and the cross-links of spectrin. The model can account for the unusual cylindrical shape of outer hair cells and suggests a mechanism of force generation based upon the elastic and electrostatic properties of spectrin.

Bibliography

Holley, M. C., & Ashmore, J. F. (1990). Spectrin, actin and the structure of the cortical lattice in mammalian cochlear outer hair cells. Journal of Cell Science, 96(2), 283â291.

Authors 2

M. C. Holley (first)
J. F. Ashmore (additional)

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{'key': '2023100623320028000_JOCES_96_2_283C11', 'first-page': '317', 'article-title': 'Actin, myosin and associated proteins in the vertebrate auditory and vestibular organs: Immunocytochemical and biochemical studies', 'volume-title': 'In Auditory Biochemistry', 'author': 'Drenckhahn', 'year': '1985'} / In Auditory Biochemistry / Actin, myosin and associated proteins in the vertebrate auditory and vestibular organs: Immunocytochemical and biochemical studies by Drenckhahn (1985)
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Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 5:30 p.m.)
Deposited	1 year, 10 months ago (Oct. 6, 2023, 7:33 p.m.)
Indexed	1 month, 1 week ago (July 16, 2025, 8:09 a.m.)
Issued	35 years, 2 months ago (June 1, 1990)
Published	35 years, 2 months ago (June 1, 1990)
Published Online	35 years, 2 months ago (June 1, 1990)
Published Print	35 years, 2 months ago (June 1, 1990)

Funders 0

None

BibTeX

@article{Holley_1990, title={Spectrin, actin and the structure of the cortical lattice in mammalian cochlear outer hair cells}, volume={96}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.96.2.283}, DOI={10.1242/jcs.96.2.283}, number={2}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Holley, M. C. and Ashmore, J. F.}, year={1990}, month=jun, pages={283–291} }

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