DOI: 10.1242/jcs.95.3.371. A 116000 <i>M</i> <i>r</i> nucleolar antigen specific for the dense fibrillar component of the nucleoli

A 116000 M r nucleolar antigen specific for the dense fibrillar component of the nucleoli

10.1242/jcs.95.3.371

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT In ATT, a human autoimmune serum, we found anti-nucleolar antibodies that recognized nucleolar antigens confined to a single nucleolar compartment, the dense fibrillar component (DFC). We localized these antigens by immunoelectron microscopy in DFC of HeLa cell nucleoli both on Lowicryl sections and cryoultrathin sections without embedding. The antigens were solubilized by incubation with 2 M NaCl but not by RNase or DNase treatment. The ATT serum crossreacted with rat liver nucleoli and PtK1 cell nucleoli in which immunofluorescence labelling displayed a clumpy pattern. During mitosis, the antigens dispersed in the cytoplasm until late telophase, when they gathered in the prenucleolar bodies. In human peripheral lymphocytes, or HeLa cells treated with actinomycin D, the antigens were still present but the fluorescence intensity decreased. By immunoblotting using human nuclear extracts, the ATT serum bound to a 116 000 Mr protein at dilutions up to 1:2000. The reactivity of this band diminished with actinomycin D-treated nuclear extracts. Two minor bands were also observed at 97 and 70K (K=103Mr). Immunopurification by competition or elution demonstrated that the 116K antigens were at the origin of the nucleolar labelling. This DFC marker appeared to be different from the NORsilver-stained proteins, which in our preparations exhibited apparent molecular weights of 105,80 and 38 –40K. In addition, these 116K antigens did not exhibit the characteristics described for DNA topoisomerase I, fibrillarin or nucleolin. We propose the 116K antigen as a new marker of the DFC of the nucleoli.

Bibliography

Masson, C., Andre, C., Arnoult, J., Geraud, G., & Hernandez-Verdun, D. (1990). A 116000 M r nucleolar antigen specific for the dense fibrillar component of the nucleoli. Journal of Cell Science, 95(3), 371â381.

Authors 5

C. Masson (first)
C. Andre (additional)
J. Arnoult (additional)
G. Geraud (additional)
D. Hernandez-Verdun (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 8:30 p.m.)
Deposited	1 year, 9 months ago (Nov. 16, 2023, 7:03 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 3:57 p.m.)
Issued	35 years, 6 months ago (March 1, 1990)
Published	35 years, 6 months ago (March 1, 1990)
Published Online	35 years, 6 months ago (March 1, 1990)
Published Print	35 years, 6 months ago (March 1, 1990)

Funders 0

None

BibTeX

@article{Masson_1990, title={A 116000 M r nucleolar antigen specific for the dense fibrillar component of the nucleoli}, volume={95}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.95.3.371}, DOI={10.1242/jcs.95.3.371}, number={3}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Masson, C. and Andre, C. and Arnoult, J. and Geraud, G. and Hernandez-Verdun, D.}, year={1990}, month=mar, pages={371–381} }

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