DOI: 10.1242/jcs.94.3.463. Persistence of major nuclear envelope antigens in an envelope-like structure during mitosis in <i>Drosophila melanogaster</i> embryos

Persistence of major nuclear envelope antigens in an envelope-like structure during mitosis in Drosophila melanogaster embryos

10.1242/jcs.94.3.463

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT Using monoclonal antibodies, we followed the fate of three different nuclear envelope proteins during mitosis in Drosophila early embryos by indirect immunofluorescence microscopy. Two of these proteins, lamin and otefin, a newly characterized nuclear envelope polypeptide with an apparent Mr of 53 000, are apparently present in an envelope-like structure that is present throughout mitosis. Immunoelectron microscopy of interphase nuclei indicates that otefin, like lamin, is not a component of nuclear pore complexes. In contrast with lamin and otefin, gpl88, a putative pore complex component, was completely redistributed through the surrounding cytoplasm during prophase in comparable early embryo specimens and was present in an envelope only in interphase. Together with previous morphological studies by other workers, these data suggest that the entire mitotic apparatus including condensed chromosomes and spindle is enclosed by an envelope throughout mitosis during early embryo-genesis in Drosophila. This ‘spindle envelope’, as it has been named by others, contains both lamin and otefin but probably not pore complex proteins.

Bibliography

Harel, A., Zlotkin, E., Nainudel-Epszteyn, S., Feinstein, N., Fisher, P. A., & Gruenbaum, Y. (1989). Persistence of major nuclear envelope antigens in an envelope-like structure during mitosis in Drosophila melanogaster embryos. Journal of Cell Science, 94(3), 463â470.

Authors 6

Amnon Harel (first)
Efrat Zlotkin (additional)
Sandra Nainudel-Epszteyn (additional)
Naomi Feinstein (additional)
Paul A. Fisher (additional)
Yosef Gruenbaum (additional)

References 34 Referenced 77

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{'key': '2023071215051802400_JOCES_94_3_463C8', 'first-page': '119', 'article-title': 'Karyoskeletal proteins of Drosophila', 'volume-title': 'In Chromosome and Chromatin Structure', 'author': 'Fisher', 'year': '1988'} / In Chromosome and Chromatin Structure / Karyoskeletal proteins of Drosophila by Fisher (1988)
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Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 5:30 p.m.)
Deposited	2 years, 1 month ago (July 12, 2023, 11:06 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 8, 2024, 4:51 a.m.)
Issued	35 years, 10 months ago (Nov. 1, 1989)
Published	35 years, 10 months ago (Nov. 1, 1989)
Published Online	35 years, 10 months ago (Nov. 1, 1989)
Published Print	35 years, 10 months ago (Nov. 1, 1989)

Funders 0

None

BibTeX

@article{Harel_1989, title={Persistence of major nuclear envelope antigens in an envelope-like structure during mitosis in Drosophila melanogaster embryos}, volume={94}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.94.3.463}, DOI={10.1242/jcs.94.3.463}, number={3}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Harel, Amnon and Zlotkin, Efrat and Nainudel-Epszteyn, Sandra and Feinstein, Naomi and Fisher, Paul A. and Gruenbaum, Yosef}, year={1989}, month=nov, pages={463–470} }

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