DOI: 10.1242/jcs.87.4.535. Visualization of the intact endoplasmic reticulum by immunofluorescence with antibodies to the major er glycoprotein, endoplasmin

Visualization of the intact endoplasmic reticulum by immunofluorescence with antibodies to the major er glycoprotein, endoplasmin

10.1242/jcs.87.4.535

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT Antibodies to endoplasmin were used to examine the morphology of the endoplasmic reticulum (ER) by immunofluorescence on permeabilized plasmacytoma and fibroblastoid cells. In unfixed cells, permeabilization led to a pronounced vésiculation of the ER. Therefore cells were first fixed lightly prior to permeabilization with detergent. Fibroblastoid cells gave a characteristic reticular pattern surrounding the nucleus with clear staining of the nuclear membrane. Plasmacytoma cells, in the conventional fluorescence microscope, gave a cistemae-like pattern. Optical sectioning with a confocal scanning laser microscope gave a distinct pattern of concentric cisternae similar to those obtained with transmission electron microscopy on cell sections. The overall morphology of the ER in such cells could be revealed by serial optical sectioning. Evidence was obtained that the ER does not undergo extensive vésiculation during mitosis in plasmacytoma cells. Using anti-endoplasmin immunofluorescence monitoring, conditions were developed for the retention of ER morphology in unfixed, permeabilized cells. These studies illustrate the value of endoplasmin as a general marker for the analysis of ER morphology in different types of cells by immunofluorescence microscopy.

Bibliography

Koch, G. L. E., Macer, D. R. J., & Smith, M. J. (1987). Visualization of the intact endoplasmic reticulum by immunofluorescence with antibodies to the major er glycoprotein, endoplasmin. Journal of Cell Science, 87(4), 535â542.

Authors 3

G. L. E. Koch (first)
D. R. J. Macer (additional)
M. J. Smith (additional)

References 17 Referenced 0

{'key': '2023111623541235600_JOCES_87_4_535C1', 'volume-title': 'Molecular Biology of the Cell', 'author': 'Alberts', 'year': '1983'} / Molecular Biology of the Cell by Alberts (1983)
10.1016/0304-4157(75)90006-4 / Biochim. biophys. Acta / Structural aspects of the membrane of the endoplasmic reticulum by De Pierre (1975)
10.1083/jcb.66.3.663 / F. Cell Biol / Mitotic activity in non-neoplastic melanocytes in vivo as determined by histochemical autoradiographic and electron microscope studies by Jimbow (1975)
10.1038/bjc.1986.3 / Br.F. Cancer S3, 13–22 / Specificity of antibodies to the purified Con A acceptor glycoproteins of cultured tumour cells by Koch (1986)
10.1242/jcs.86.1.217 / F. Cell Sci / Endoplasmic reticulum contains a common abundant calcium-binding glycoprotein, endoplasmin by Koch (1986)
{'key': '2023111623541235600_JOCES_87_4_535C6', 'volume-title': 'Ultrastructure of the Mammalian Cell', 'author': 'Krstic', 'year': '1979'} / Ultrastructure of the Mammalian Cell by Krstic (1979)
10.1083/jcb.92.1.92 / F. Cell Biol / Antibodies to the Golgi complex and the rough endoplasmic reticulum by Louvard (1982)
10.1242/jcs.12.1.163 / F. Cell Sci / An ultrastructural study of the pancreatic acinar cell in mitosis, with special reference to changes in the Golgi complex by Melmed (1973)
{'key': '2023111623541235600_JOCES_87_4_535C9', 'first-page': '641', 'volume': '100', 'author': 'Palade', 'year': '1954', 'journal-title': 'Studies on the endoplasmic reticulum. I. Its identification in cells in situ. F. exp. Med'} / Studies on the endoplasmic reticulum. I. Its identification in cells in situ. F. exp. Med by Palade (1954)
10.1016/0012-1606(72)90083-8 / Devi Biol / An ultrastructural analysis of the developing embryonic pancreas by Pictet (1972)
10.1084/jem.97.5.727 / F. exp. Med / Observations on the submicroscopic basophilic component of the cytoplasm by Porter (1953)
10.1084/jem.81.3.233 / F. exp. Med / A study of tissue culture cells by electron microscopy by Porter (1945)
10.1083/jcb.46.1.81 / F. Cell Biol / Proliferative behaviour of differentiating cells in the developing rat parotid gland by Redman (1970)
10.1083/jcb.21.3.429 / F. Cell Biol / The ultrastructure of a mammalian cell during the mitotic cycle by Robbins (1964)
10.1016/0092-8674(84)90530-0 / Cell / Localisation of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes by Terasaki (1984)
10.1083/jcb.17.2.299 / F. Cell Biol / Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines by Todaro (1963)
10.1016/S0022-5320(79)80065-9 / F. Ultrastruct. Res / Mitosis in rat thyroid epithelial cells in vivo. Ultrastructural changes in cytoplasmic organelles during the mitotic cycle by Zeligs (1979)

Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 1:13 p.m.)
Deposited	1 year, 9 months ago (Nov. 16, 2023, 1:54 p.m.)
Indexed	1 year, 9 months ago (Nov. 16, 2023, 10:10 p.m.)
Issued	38 years, 3 months ago (May 1, 1987)
Published	38 years, 3 months ago (May 1, 1987)
Published Online	38 years, 3 months ago (May 1, 1987)
Published Print	38 years, 3 months ago (May 1, 1987)

Funders 0

None

BibTeX

@article{Koch_1987, title={Visualization of the intact endoplasmic reticulum by immunofluorescence with antibodies to the major er glycoprotein, endoplasmin}, volume={87}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.87.4.535}, DOI={10.1242/jcs.87.4.535}, number={4}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Koch, G. L. E. and Macer, D. R. J. and Smith, M. J.}, year={1987}, month=may, pages={535–542} }

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