DOI: 10.1242/jcs.67.1.69. The Major 67 000 Molecular Weight Protein Of The Clam Oocyte Nuclear Envelope Is Lamin-Like

The Major 67 000 Molecular Weight Protein Of The Clam Oocyte Nuclear Envelope Is Lamin-Like

10.1242/jcs.67.1.69

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT Nuclear envelopes of somatic cells have at least two different major proteins in the 60—70(×103) molecular weight range (1amins A(C) and B) that seem to be involved in chromatin attachment. In contrast, nuclear envelopes from clam germinal vesicles have only a single major protein of the same size class (∽67×103Mr) and have no chromatin attached to them. The data presented in this report show that this 67×103μ, clam protein shares a variety of physical properties with lamins A(C) and B, derived from rat liver nuclei. These properties include similar size, although different isoelectric points; phosphorylated forms; strong tendencies to cross-link by disulphide bonds; presence of carbohydrates, demonstrated by direct incorporation of mannose and labelling with borohydride; and shared epitopes, demonstrated using both monoclonal and polyclonal antibodies. Taken together, these observations identify the clam 67× 10Mr protein, the major structural protein of a nuclear envelope that lacks attached chromatin, as being lamin-like and demonstrate that it is more closely related to lamin A(C) than to lamin B.

Bibliography

Maul, G. G., Baglia, F. A., Newmeyer, D. D., & Ohlsson-Wilhelm, B. M. (1984). The Major 67 000 Molecular Weight Protein Of The Clam Oocyte Nuclear Envelope Is Lamin-Like. Journal of Cell Science, 67(1), 69â85.

Authors 4

Gerd G. Maul (first)
Frank A. Baglia (additional)
Donald D. Newmeyer (additional)
Betsy M. Ohlsson-Wilhelm (additional)

References 27 Referenced 28

10.1073/pnas.73.9.3243 / Proc. natn. Acad. Set. U.SA. / Isolation of homozygous mutants after induced self-fertilization in Tetrahymena by Bruns (1976)
{'key': '2023070504151311300_JOCES_67_1_69C2', 'first-page': '315', 'article-title': 'A study of the conditions and mechanism of the diphenylamine reaction for the colorometric estimation of deoxyribonucleic acid', 'volume': '62', 'author': 'Burton', 'year': '1956', 'journal-title': 'Biochemistry'} / Biochemistry / A study of the conditions and mechanism of the diphenylamine reaction for the colorometric estimation of deoxyribonucleic acid by Burton (1956)
10.1111/j.1550-7408.1979.tb02725.x / J. Protozoal. / Regulation of macronuclear DNA content in Paramecium lelraurelia by Berger (1979)
10.1016/0014-4827(78)90520-7 / Expl Cell Res. / The relationship between cell size and cell division in the yeast Saccharomyces cerevisiae by Carter (1978)
10.1016/0014-4827(68)90407-2 / Expl Cell Res. / Regulierung der DNS Menge im Makronukleus von Tetrahymena by Cleffmann (1968)
10.1242/jcs.37.1.117 / J. Cell Sci. / Increase in macromolecular amounts during the cell cycle of Tetrahymena: A contribution to cell cycle control by Cleffmann (1979)
10.1002/jez.1402090107 / J. exp. Zool. / Dependence of oral development and cleavage on cell size in the ciliate Stentor by DeTerra (1979)
10.1242/jcs.24.1.51 / J. Cell Sci. / Control of cell size and cycle time in Schizosaccharomyces pombe by Fantes (1977)
10.1016/0014-4827(77)90359-7 / Expl Cell Res. / Control of the size at division in fission yeast by a growth- modulated size control over nuclear division by Fantes (1977)
10.1016/0014-4827(65)90285-5 / Expl Cell Res. / A quantitative cytochemical investigation of the relationship between cell mass and initiation of DNA synthesis in mouse fibroblasts in vitro by Killander (1965)
10.1128/jb.143.3.1241-1252.1980 / J. Bact. / Correlation between size and age at different events in the cell division cycle of Escherichia coli by Koppes (1980)
10.1016/S0021-9258(19)52451-6 / J. biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry (1951)
{'key': '2023070504151311300_JOCES_67_1_69C13', 'volume-title': 'The Biology of the Cell Cycle.', 'author': 'Mitchison', 'year': '1971'} / The Biology of the Cell Cycle. by Mitchison (1971)
{'key': '2023070504151311300_JOCES_67_1_69C14', 'first-page': '114', 'article-title': 'The determination of nucleic acids', 'volume': '14', 'author': 'Munro', 'year': '1966', 'journal-title': 'Meth, biochem. Anal.'} / Meth, biochem. Anal. / The determination of nucleic acids by Munro (1966)
10.2307/3225867 / Am. Microsc. Sac. / The protargol staining technique: an improved version for Tetrahymena pyriformis by Ng (1977)
10.1016/0014-4827(77)90358-5 / Expl Cell Res. / Controls over the timing of DNA replication during the cellcycle of fission yeast by Nurse (1977)
{'key': '2023070504151311300_JOCES_67_1_69C17', 'volume-title': 'Reproduction of Eukarydie Cells.', 'author': 'Prescott', 'year': '1976'} / Reproduction of Eukarydie Cells. by Prescott (1976)
{'key': '2023070504151311300_JOCES_67_1_69C18', 'article-title': 'The protozoan nucleus. In Cell Biol', 'volume': '9', 'author': 'Raikov', 'year': '1982', 'journal-title': 'Monographs,'} / Monographs, / The protozoan nucleus. In Cell Biol by Raikov (1982)
{'key': '2023070504151311300_JOCES_67_1_69C19', 'first-page': '211', 'article-title': 'Aspects of cell size measurements in Tetrahymena elliotti', 'volume': '120', 'author': 'Roberts', 'year': '1980', 'journal-title': 'J. gen. Microbiol.'} / J. gen. Microbiol. / Aspects of cell size measurements in Tetrahymena elliotti by Roberts (1980)
10.1016/0014-4827(82)90028-3 / Expl Cell Res. / Division and growth kinetics of the division mutant conical of Tetrahymena: A contribution to regulation of generation time by SchAfer (1982)
10.1242/jcs.58.1.211 / J. Cell Sci. / Mean macronuclear DNA contents are variable in the ciliate Tetrahymena by Seyfert (1982)
10.1242/jcs.40.1.111 / J. Cell Sci. / The regulation of amounts and proportions of genetics elements in the macronucleus of Tetrahymena thermophila strains of diverse karyotype by Seyfert (1979)
10.1016/0092-8674(78)90016-8 / Cell / Cell size, cell cycle and transition probability in mouse fibroblasts by Shields (1978)
10.1016/0047-6374(75)90028-7 / Meeh. Aging Devel. / Age dependent micronuclear deterioration in Tetrahymena pyriformis, syngen I by Weidenruch (1975)
{'key': '2023070504151311300_JOCES_67_1_69C25', 'first-page': '119', 'article-title': 'Nucleocytoplasmic ratio requirements for the initiation of DNA replication and fission in Tetrahymena', 'volume': '9', 'author': 'Worthington', 'year': '1976', 'journal-title': 'Cell Tiss. Kinet'} / Cell Tiss. Kinet / Nucleocytoplasmic ratio requirements for the initiation of DNA replication and fission in Tetrahymena by Worthington (1976)
10.1016/0014-4827(83)90454-8 / Expl Cell Res. / Induction of DNA synthesis and mitosis in the absence of cellular enlargement by Zetterberg (1983)
10.1016/0014-4827(70)90453-2 / Expl Cell Res. / Independent synchronisation of DNA synthesis and of cell division in the same culture of Tetrahymena cells by Zeuthen (1970)

Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 4:58 p.m.)
Deposited	2 years, 1 month ago (July 5, 2023, 12:15 a.m.)
Indexed	1 year, 10 months ago (Oct. 22, 2023, 9:28 p.m.)
Issued	41 years, 4 months ago (April 1, 1984)
Published	41 years, 4 months ago (April 1, 1984)
Published Online	41 years, 4 months ago (April 1, 1984)
Published Print	41 years, 4 months ago (April 1, 1984)

Funders 0

None

BibTeX

@article{Maul_1984, title={The Major 67 000 Molecular Weight Protein Of The Clam Oocyte Nuclear Envelope Is Lamin-Like}, volume={67}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.67.1.69}, DOI={10.1242/jcs.67.1.69}, number={1}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Maul, Gerd G. and Baglia, Frank A. and Newmeyer, Donald D. and Ohlsson-Wilhelm, Betsy M.}, year={1984}, month=apr, pages={69–85} }

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  "published": {
    "date-parts": [
      [
        1984,
        4,
        1
      ]
    ]
  }
}