DOI: 10.1242/jcs.48.1.383. Repair of strand breaks in superhelical dna of ataxia telangiectasia lymphoblastoid cells

Repair of strand breaks in superhelical dna of ataxia telangiectasia lymphoblastoid cells

10.1242/jcs.48.1.383

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT A number of different assay methods have been used to study repair of strand breaks in DNA after exposure of cells to ionizing radiation. Use of these methods indicates that fibroblasts from patients with ataxia telangiectasia (AT), a multiform genetic disease exhibiting high sensitivity to ionizing radiation, have a normal ability to repair strand breaks in DNA. All of these methods determine the extent of breakage of DNA and the resealing of these breaks but do not provide information on restoration of DNA configuration in the nucleus. In this report we have used a sensitive technique to investigate restoration of the 3-dimensional structure of DNA in AT lymphoblastoid cells after exposure to ionizing radiation. This technique provides a means of lysing cells using a high concentration of salt and a non-ionic detergent, giving rise to structures called nucleoids which contain nuclear RNA and DNA, are depleted in protein, and sediment in a manner characteristic of supercoiled DNA. We have shown that the degree of supercoiling is the same in control and AT lymphoblastoid cells using sedimentation in the presence of ethidium bromide. The extent of breakage after exposure of cells to y-radiation, and the rate of repair of these breaks are similar in both cell types. Rate of repair of strand breaks is dose dependent and the restructured rapidly sedimenting complex behaves similarly, on sucrose gradients containing ethidium bromide, to that extracted from unirradiated cells.

Bibliography

Lavin, M. F., & Davidson, M. (1981). Repair of strand breaks in superhelical dna of ataxia telangiectasia lymphoblastoid cells. Journal of Cell Science, 48(1), 383â391.

Authors 2

M. F. Lavin (first)
M. Davidson (additional)

References 20 Referenced 27

10.1146/annurev.ge.12.120178.000523 / A. Rev. Genet. / Human disorders showing increased sensitivity to the induction of genetic damage by Arlett (1978)
10.1038/274484a0 / Nature, Land. / Identification of ataxia telangiectasia heterozygotes, a cancer prone population by Chen (1978)
{'key': '2023053021583075900_JOCES_48_1_383C3', 'first-page': '261', 'article-title': 'Supercoils in human DNA', 'volume': '19', 'author': 'Cook', 'year': '1975', 'journal-title': 'F. Cell Set’.'} / F. Cell Set’. / Supercoils in human DNA by Cook (1975)
10.1038/263679a0 / Nature, Land. / Detection and repair of single-strand breaks in nuclear DNA by Cook (1976)
{'key': '2023053021583075900_JOCES_48_1_383C5', 'first-page': '303', 'article-title': 'Characterization of nuclear structures containing superhelical DNA', 'volume': 'aa', 'author': 'Cook', 'year': '1976', 'journal-title': 'F. Cell Sri.'} / F. Cell Sri. / Characterization of nuclear structures containing superhelical DNA by Cook (1976)
10.1073/pnas.73.3.809 / Proc. natn. Acad. Sci. U.S.A / DNA strand breaks, repair, and survival in X-irradiated mammalian cells by Dugle (1976)
{'key': '2023053021583075900_JOCES_48_1_383C7', 'first-page': '61', 'article-title': 'Genes which increase chromosomal instability in somatic cells and predispose to cancer', 'volume': '8', 'author': 'German', 'year': '1972', 'journal-title': 'Prog. med. Genet.'} / Prog. med. Genet. / Genes which increase chromosomal instability in somatic cells and predispose to cancer by German (1972)
10.1093/nar/8.16.3709 / Nucleic Acids Res. / Effect of ionizing radiation on DNA synthesis in ataxia telangiectasia cells by Houldsworth (1980)
10.1016/B978-0-12-322650-1.50089-8 / In DNA Repair Mechanisms by Hutchinson (1978)
10.1016/0027-5107(70)90036-9 / Mutation Ret. / Repair of X-ray damage in DNA of cultivated cells from patients having xeroderma pigmentosum by Kleijer (1970)
10.1016/0090-1229(78)90188-5 / Clin. Immun. Immunopath. / Lymphocytes in ataxia telangiectasia: Implications of the DNA repair defect by Lavin (1978)
10.1016/0005-2787(77)90213-1 / Biochim. biophys. Acta / The production and repair of double strand breaks in cells from normal humans and from patients with ataxia telangiectasia by Lehmann (1977)
{'key': '2023053021583075900_JOCES_48_1_383C13', 'first-page': '67', 'volume-title': 'In Biology of Radiation Carcinogenesis', 'author': 'Ormerod', 'year': '1976'} / In Biology of Radiation Carcinogenesis by Ormerod (1976)
10.1038/260444a0 / Nature, Land. / Defective excision repair of y-ray-damaged DNA in human (ataxia telangiectasia) fibroblasts by Paterson (1976)
10.1093/nar/4.2.299 / Nucleic Acids Res. / Single strand breakage and repair in eukaryotic DNA as assayed by Si nuclease by Sheridan (1977)
10.1016/0027-5107(79)90148-9 / Mutation Res. / Ataxia telangiectasia: Further considerations of the evidence for single strand break repair by Sheridan (1979)
10.1016/0027-5107(78)90045-3 / Mutation Res. / Unrepaired DNA strand breaks in irradiated ataxia telangiectasia lymphocytes suggested from cytogenetic observations by Taylor (1978)
10.1038/258427a0 / Nature, Lond. / Ataxia telangiectasia: A human mutation with abnormal radiation sensitivity by Taylor (1975)
10.1016/0027-5107(75)90211-0 / Mutation Res. / DNA strand breakage repair in ataxia telangiectasia fibroblast-like cells by Vincent (1975)
10.1016/B978-0-12-035405-4.50011-6 / Adv. Radiat. Biol. / Molecular mechanisms of radiation-induced damage to nucleic acids by Ward (1975)

Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 4:54 p.m.)
Deposited	2 years, 3 months ago (May 30, 2023, 5:58 p.m.)
Indexed	1 year, 10 months ago (Oct. 31, 2023, 2:46 p.m.)
Issued	44 years, 4 months ago (April 1, 1981)
Published	44 years, 4 months ago (April 1, 1981)
Published Online	44 years, 4 months ago (April 1, 1981)
Published Print	44 years, 4 months ago (April 1, 1981)

Funders 0

None

BibTeX

@article{Lavin_1981, title={Repair of strand breaks in superhelical dna of ataxia telangiectasia lymphoblastoid cells}, volume={48}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.48.1.383}, DOI={10.1242/jcs.48.1.383}, number={1}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Lavin, M. F. and Davidson, M.}, year={1981}, month=apr, pages={383–391} }

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