DOI: 10.1242/jcs.21.1.119. Subcellular localization of diffusible ions in the yeast <i>Saccharomyces Cerevisiae:</i> quantitative microprobe analysis of thin freeze-dried sections

Subcellular localization of diffusible ions in the yeast Saccharomyces Cerevisiae: quantitative microprobe analysis of thin freeze-dried sections

10.1242/jcs.21.1.119

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT In yeast cells, of which the intracellular potassium had been partly replaced by rubidium or caesium, the intracellular ion distribution was studied by means of energy-dispersive X-ray microanalysis. The cells were rapidly frozen and thin sections were cut at low temperature on a cryo-ultramicrotome without the use of a trough liquid. By this dry cryosectioning procedure, complete retention of the diffusible ions in the cells was obtained. Unless the sections had been exposed to moisture, no signs of redistribution were apparent. For quantitative determinations a gelatin standard, containing known amounts of the elements of interest, was prepared in the same way as the cells. The concentrations of potassium, rubidium, caesium and chloride in the nucleus, the cytoplasm and the vacuole could be measured. The intracellular distributions of potassium, rubidium and caesium were very similar. The concentrations of these ions in the cytoplasm were about equal to those in the nucleus and twice those in the vacuole. The total concentration in the cytoplasm was 180–190 mmol/kg fresh weight, in the nucleus 190—200 mmol/kg fresh weight and in the vacuole 75–0 mmol/kg fresh weight. The permeability of the yeast cell for chloride is markedly lower than for the cations.

Bibliography

Roomans, G. M., & SevÃ©us, L. A. (1976). Subcellular localization of diffusible ions in the yeast Saccharomyces Cerevisiae: quantitative microprobe analysis of thin freeze-dried sections. Journal of Cell Science, 21(1), 119â127.

Authors 2

G. M. Roomans (first)
L. A. Sevéus (additional)

References 30 Referenced 38

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10.1111/j.1438-8677.1974.tb00965.x / Acta bot. neerl / Net Rb+ transport by Rb+ loaded cells and the electrokinetical formulism by Azoulay (1974)
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{'key': '2023062603471634200_JOCES_21_1_119C7', 'first-page': '89', 'article-title': 'The kinetics of Rb+ uptake by Saccharomyces cerevisiae', 'volume': '2', 'author': 'Borst-Pauwels', 'year': '1974', 'journal-title': 'Proc. 4th Int. Yeast Symp., Vienna'} / Proc. 4th Int. Yeast Symp., Vienna / The kinetics of Rb+ uptake by Saccharomyces cerevisiae by Borst-Pauwels (1974)
10.1016/0005-2736(71)90220-3 / Biochim. biophys. Acta / The interaction of 2,4-dinitrophenol with anaerobic Rb+ transport across the yeast cell membrane by Borst-Pauwels (1971)
10.1242/jcs.7.1.5 / J. Cell Sci / The concentrations of water, sodium and potassium in the nucleus and cytoplasm of amphibian oocytes by Century (1970)
10.1038/159137a0 / Nature, Lond / Mechanism of high acid production by yeast and its bearing on hydrochloric acid formation in the stomach by Conway (1947)
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{'key': '2023062603471634200_JOCES_21_1_119C14', 'first-page': '147', 'article-title': 'Measurement of mass loss in biological specimens under an electron microbeam', 'volume-title': 'In Microprobe Analysis as Applied to Cells and Tissues', 'author': 'Hall', 'year': '1974'} / In Microprobe Analysis as Applied to Cells and Tissues / Measurement of mass loss in biological specimens under an electron microbeam by Hall (1974)
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10.1071/BI9731015 / Aust. J. biol. Sci / Electron probe microanalysis of potassium and chloride in freeze substituted leaf sections of Zea mays by Pallaghy (1973)
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{'key': '2023062603471634200_JOCES_21_1_119C25', 'first-page': '55', 'article-title': 'X-ray microanalysis in the biological sciences', 'volume': '6', 'author': 'Russ', 'year': '1974', 'journal-title': 'J. submicrosc. Cytol'} / J. submicrosc. Cytol / X-ray microanalysis in the biological sciences by Russ (1974)
{'key': '2023062603471634200_JOCES_21_1_119C26', 'first-page': '52', 'article-title': 'Dry cryosectioning of human and animal tissue at very low temperature (–140 °C)', 'volume': '2', 'author': 'Sevéus', 'year': '1974', 'journal-title': 'Proc. 3th Int. Congr. Electron Microsc., Canberra'} / Proc. 3th Int. Congr. Electron Microsc., Canberra / Dry cryosectioning of human and animal tissue at very low temperature (–140 °C) by Sevéus (1974)
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10.1016/0022-5193(76)90004-7 / J. theor. Biol / The influence of surface charge on the kinetics of ion-translocation across biological membranes by Theuvenet (1976)
{'key': '2023062603471634200_JOCES_21_1_119C30', 'first-page': '393', 'article-title': 'Improved contrast in dry ultrathin frozen sections', 'volume-title': 'In Electron Microscopy and Cytochemistry', 'author': 'Werner', 'year': '1973'} / In Electron Microscopy and Cytochemistry / Improved contrast in dry ultrathin frozen sections by Werner (1973)

Dates

Type	When
Created	4 years, 3 months ago (April 24, 2021, 1:17 a.m.)
Deposited	2 years, 1 month ago (June 25, 2023, 11:47 p.m.)
Indexed	1 year, 9 months ago (Nov. 10, 2023, 8:41 p.m.)
Issued	49 years, 2 months ago (June 1, 1976)
Published	49 years, 2 months ago (June 1, 1976)
Published Online	49 years, 2 months ago (June 1, 1976)
Published Print	49 years, 2 months ago (June 1, 1976)

Funders 0

None

BibTeX

@article{Roomans_1976, title={Subcellular localization of diffusible ions in the yeast Saccharomyces Cerevisiae: quantitative microprobe analysis of thin freeze-dried sections}, volume={21}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.21.1.119}, DOI={10.1242/jcs.21.1.119}, number={1}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Roomans, G. M. and Sevéus, L. A.}, year={1976}, month=jun, pages={119–127} }

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