DOI: 10.1242/jcs.1993.supplement_17.21. Characterization of recombinant E-cadherin (uvomorulin) expressed in insect cells

Characterization of recombinant E-cadherin (uvomorulin) expressed in insect cells

10.1242/jcs.1993.supplement_17.21

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT Cadherins are Ca2+-dependent cell adhesion molecules that mediate cell adhesion by homophilic binding. Structural and functional analysis of the extracellular part of cadherins that mediates this binding has often been hampered by the availability of sufficient amount of protein. Therefore, we have expressed the extracellular region of E-cadherin (uvomorulin) using the baculovirus expression vector system (BEVS). A recombinant baculovirus was generated that encodes the signal peptide, the precursor region and the extracellular part of the mature protein, under the control of the promotor for polyhedrin. Infection of insect cells with recombinant virus led to the expression of about 40 mg of the E-cadherin fragment per 2×109 infected cells. About half of the protein synthesized was secreted, either as mature protein or in its unprocessed form. The precursor peptide was removed by trypsin treatment in the presence of Ca2+ and recombinant protein was purified to homogeneity. Biochemical characterization of the recombinant protein revealed a high degree of similarity with the mouse wild-type protein. Recombinant protein exhibited the known resistance to trypsin in the presence of Ca2+ and was recognized by two different conformation-sensitive monoclonal anti-E-cadherin antibodies. Rabbit antibodies made against the recombinant protein recognized E-cadherin from different species. In spite of the high degree of structural resemblance recombinant E-cadherin was not able to inhibit E-cadherin mediated cell-cell adhesion.

Bibliography

Herrenknecht, K., & Kemler, R. (1993). Characterization of recombinant E-cadherin (uvomorulin) expressed in insect cells. Journal of Cell Science, 1993(Supplement_17), 147â154.

Authors 2

Kurt Herrenknecht (first)
Rolf Kemler (additional)

References 31 Referenced 14

10.1016/0012-1606(90)90290-Y / Dev. Biol / Identification of a cadherin cell adhesion recognition sequence. by Blaschuk (1990)
10.1002/elps.1150080203 / Electrophoresis / Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. by Blum (1987)
{'key': '2024071020074959200_JOCES_1993_S17_147C3', 'first-page': '655671', 'article-title': 'Isolation and characterization of mosquito cell membrane glycoproteins.', 'volume': '640', 'author': 'Butters', 'year': '1981', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta / Isolation and characterization of mosquito cell membrane glycoproteins. by Butters (1981)
10.1016/0005-2736(81)90097-3 / Biochim. Biophys. Acta / Steps in the biosynthesis of mosquito cell membrane glycoproteins and the effects of tunicamycin. by Butters (1981)
10.1083/jcb.106.3.873 / J. Cell Biol / Cloning and expression of cDNA encoding a neural calcium-de-pendent cell adhesion molecule: Its identity in the cadherin gene family. by Hatta (1988)
{'key': '2024071020074959200_JOCES_1993_S17_147C6', 'volume-title': 'Manipulating the Mouse Embryo. A Laboratory Manual', 'author': 'Hogan', 'year': '1986'} / Manipulating the Mouse Embryo. A Laboratory Manual by Hogan (1986)
10.1016/S0021-9258(17)43362-X / J. Biol. Chem / Regulation of apsaragine-linked oligosaccharide processing. by Hsieh (1984)
10.1016/0092-8674(81)90214-2 / Cell / Cell-cell interactions in early embryogenesis: a molecular approach to the role of calcium. by Hyafil (1981)
10.1016/0092-8674(92)90472-O / Cell / Contact and adhesive specifities in the associations, migrations and targeting of cells and axons. by Hynes (1992)
10.1016/0955-0674(89)90055-0 / Curr. Opin. Cell Biol / Calcium-dependent cell adhesion molecules. by Kemler (1989)
10.1016/S1043-4682(10)80011-X / Semin. Cell Biol / Classical cadherins. by Kemler (1992)
10.1146/annurev.bi.54.070185.003215 / Rev. Biochem / Assembly of asparagine-linked oligosaccharides. Ann". by Kornfeld (1985)
10.1016/S0959-437X(05)80348-X / Curr. Opin. Gen. Dev / Baculoviruses: high level expression in insect cells. by Miller (1993)
10.1038/329341a0 / Nature / Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA. by Nagafuchi (1987)
{'key': '2024071020074959200_JOCES_1993_S17_147C15', 'first-page': '3', 'article-title': 'Teratocareinome de la souris: Differentiation en culture d’un lignee de cellules primitives a potentialités multiple.', 'volume': '126 A', 'author': 'Nicholas', 'year': '1975', 'journal-title': 'Ann. Microbiol. (Inst. Pasteur)'} / Ann. Microbiol. (Inst. Pasteur) / Teratocareinome de la souris: Differentiation en culture d’un lignee de cellules primitives a potentialités multiple. by Nicholas (1975)
10.1016/0092-8674(90)90222-Z / Cell / Localization of specifity determining sites in cadherin cell adhesion molecules. by Nose (1990)
10.1002/j.1460-2075.1989.tb03563.x / EMBO J / The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species. by Ozawa (1989)
10.1016/0092-8674(90)90506-A / Cell / Single amino acid substitutions in one Ca2+ binding site of uvomorulin abolish the adhesive function. by Ozawa (1990)
10.1083/jcb.111.4.1645 / J. Cell Biol / Correct proteolytic cleavage is required for the cell adhesive function of uvomorulin. by Ozawa (1990)
{'key': '2024071020074959200_JOCES_1993_S17_147C20', 'first-page': '49', 'article-title': 'A possible new adhesive site in the cell-adhesion molecule uvomorulin.', 'volume': '33', 'author': 'Ozawa', 'year': '1991', 'journal-title': 'Meeh. Dev'} / Meeh. Dev / A possible new adhesive site in the cell-adhesion molecule uvomorulin. by Ozawa (1991)
10.1093/nar/17.1.451 / Nucl. Acids Res / An efficient procedure for the isolation of recombinant baculovirus. by Pen (1989)
10.1002/j.1460-2075.1987.tb02697.x / EMBO J / The structure of cell adhesion molecule uvomorulin. Insights into the molecular mechanism of Ca2+-dependent cell adhesion. by Ringwald (1987)
{'key': '2024071020074959200_JOCES_1993_S17_147C23', 'volume-title': 'Molecular Cloning - A Laboratory Manual, 2nd edn', 'author': 'Sambrook', 'year': '1989'} / Molecular Cloning - A Laboratory Manual, 2nd edn by Sambrook (1989)
10.1247/csf.11.245 / Cell Struct. Fund / N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin. by Shirayoshi (1986)
{'key': '2024071020074959200_JOCES_1993_S17_147C25', 'volume-title': 'A Manual Of Methods For Baculovirus Vectors And Insect Cell Culture Procedures: Bulletin no. 1555, Texas Agricultural Experimental Station, College Station, TX 77843', 'author': 'Summers', 'year': '1987'} / A Manual Of Methods For Baculovirus Vectors And Insect Cell Culture Procedures: Bulletin no. 1555, Texas Agricultural Experimental Station, College Station, TX 77843 by Summers (1987)
10.1242/dev.102.4.639 / Development / The cadherins: cell-cell adhesion molecules controlling animal morphogenesis. by Takeichi (1988)
10.1007/BF02615077 / In Vitro / The establishment of two cell lines from the insect Spodoptera frugiperda (lepidoptera: noctuidae). by Vaughn (1977)
10.1016/0014-4827(84)90241-6 / Exp. Cell Res / Rabbit antiserum against a purified surface glycoprotein decompacts mouse preimplantation embryos and reacts with specific adult tissues. by Vestweber (1984)
10.1016/0045-6039(84)90084-8 / Cell Differ / Some structural and functional aspects of the cell adhesion molecule uvomorulin. by Vestweber (1984)
10.1002/j.1460-2075.1985.tb04095.x / EMBOJ / Identification of a putative cell adhesion domain of uvomorulin. by Vestweber (1985)
{'key': '2024071020074959200_JOCES_1993_S17_147C31', 'first-page': '187', 'article-title': 'Soluble 80-kd fragment of cell-CAM 120/80 disrupts cell-cell adhesion.', 'volume-title': 'J. Cell Biochem', 'author': 'Wheelock', 'year': '1987'} / J. Cell Biochem / Soluble 80-kd fragment of cell-CAM 120/80 disrupts cell-cell adhesion. by Wheelock (1987)

Dates

Type	When
Created	12 years, 3 months ago (May 23, 2013, 8:48 a.m.)
Deposited	1 year, 1 month ago (July 10, 2024, 4:08 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 8:02 p.m.)
Issued	31 years, 9 months ago (Dec. 1, 1993)
Published	31 years, 9 months ago (Dec. 1, 1993)
Published Online	31 years, 9 months ago (Dec. 1, 1993)
Published Print	31 years, 9 months ago (Dec. 1, 1993)

Funders 0

None

BibTeX

@article{Herrenknecht_1993, title={Characterization of recombinant E-cadherin (uvomorulin) expressed in insect cells}, volume={1993}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.1993.supplement_17.21}, DOI={10.1242/jcs.1993.supplement_17.21}, number={Supplement_17}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Herrenknecht, Kurt and Kemler, Rolf}, year={1993}, month=dec, pages={147–154} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        11
      ]
    ],
    "date-time": "2024-07-11T00:02:18Z",
    "timestamp": 1720656138325
  },
  "reference-count": 31,
  "publisher": "The Company of Biologists",
  "issue": "Supplement_17",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1993,
            12,
            1
          ]
        ],
        "date-time": "1993-12-01T00:00:00Z",
        "timestamp": 754704000000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://www.biologists.com/user-licence-1-1/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1993,
        12,
        1
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n               <jats:p>Cadherins are Ca2+-dependent cell adhesion molecules that mediate cell adhesion by homophilic binding. Structural and functional analysis of the extracellular part of cadherins that mediates this binding has often been hampered by the availability of sufficient amount of protein. Therefore, we have expressed the extracellular region of E-cadherin (uvomorulin) using the baculovirus expression vector system (BEVS). A recombinant baculovirus was generated that encodes the signal peptide, the precursor region and the extracellular part of the mature protein, under the control of the promotor for polyhedrin. Infection of insect cells with recombinant virus led to the expression of about 40 mg of the E-cadherin fragment per 2\u00d7109 infected cells. About half of the protein synthesized was secreted, either as mature protein or in its unprocessed form. The precursor peptide was removed by trypsin treatment in the presence of Ca2+ and recombinant protein was purified to homogeneity. Biochemical characterization of the recombinant protein revealed a high degree of similarity with the mouse wild-type protein. Recombinant protein exhibited the known resistance to trypsin in the presence of Ca2+ and was recognized by two different conformation-sensitive monoclonal anti-E-cadherin antibodies. Rabbit antibodies made against the recombinant protein recognized E-cadherin from different species. In spite of the high degree of structural resemblance recombinant E-cadherin was not able to inhibit E-cadherin mediated cell-cell adhesion.</jats:p>",
  "DOI": "10.1242/jcs.1993.supplement_17.21",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2013,
        5,
        23
      ]
    ],
    "date-time": "2013-05-23T12:48:12Z",
    "timestamp": 1369313292000
  },
  "page": "147-154",
  "source": "Crossref",
  "is-referenced-by-count": 14,
  "title": "Characterization of recombinant E-cadherin (uvomorulin) expressed in insect cells",
  "prefix": "10.1242",
  "volume": "1993",
  "author": [
    {
      "given": "Kurt",
      "family": "Herrenknecht",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Rolf",
      "family": "Kemler",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Max-Planck-Institut f\u00fcr Immunbiologie, St\u00fcbeweg 51, D-79108 Freiburg, Germany"
        }
      ]
    }
  ],
  "member": "237",
  "published-online": {
    "date-parts": [
      [
        1993,
        12,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C1",
      "doi-asserted-by": "crossref",
      "first-page": "227",
      "DOI": "10.1016/0012-1606(90)90290-Y",
      "article-title": "Identification of a cadherin cell adhesion recognition sequence.",
      "volume": "139",
      "author": "Blaschuk",
      "year": "1990",
      "journal-title": "Dev. Biol"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C2",
      "doi-asserted-by": "crossref",
      "first-page": "93",
      "DOI": "10.1002/elps.1150080203",
      "article-title": "Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels.",
      "volume": "8",
      "author": "Blum",
      "year": "1987",
      "journal-title": "Electrophoresis"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C3",
      "first-page": "655671",
      "article-title": "Isolation and characterization of mosquito cell membrane glycoproteins.",
      "volume": "640",
      "author": "Butters",
      "year": "1981",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C4",
      "doi-asserted-by": "crossref",
      "first-page": "672",
      "DOI": "10.1016/0005-2736(81)90097-3",
      "article-title": "Steps in the biosynthesis of mosquito cell membrane glycoproteins and the effects of tunicamycin.",
      "volume": "640",
      "author": "Butters",
      "year": "1981",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C5",
      "doi-asserted-by": "crossref",
      "first-page": "873881",
      "DOI": "10.1083/jcb.106.3.873",
      "article-title": "Cloning and expression of cDNA encoding a neural calcium-de-pendent cell adhesion molecule: Its identity in the cadherin gene family.",
      "volume": "106",
      "author": "Hatta",
      "year": "1988",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C6",
      "volume-title": "Manipulating the Mouse Embryo. A Laboratory Manual",
      "author": "Hogan",
      "year": "1986"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C7",
      "doi-asserted-by": "crossref",
      "first-page": "2375",
      "DOI": "10.1016/S0021-9258(17)43362-X",
      "article-title": "Regulation of apsaragine-linked oligosaccharide processing.",
      "volume": "259",
      "author": "Hsieh",
      "year": "1984",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C8",
      "doi-asserted-by": "crossref",
      "first-page": "447454",
      "DOI": "10.1016/0092-8674(81)90214-2",
      "article-title": "Cell-cell interactions in early embryogenesis: a molecular approach to the role of calcium.",
      "volume": "26",
      "author": "Hyafil",
      "year": "1981",
      "journal-title": "Cell"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C9",
      "doi-asserted-by": "crossref",
      "first-page": "303322",
      "DOI": "10.1016/0092-8674(92)90472-O",
      "article-title": "Contact and adhesive specifities in the associations, migrations and targeting of cells and axons.",
      "volume": "68",
      "author": "Hynes",
      "year": "1992",
      "journal-title": "Cell"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C10",
      "doi-asserted-by": "crossref",
      "first-page": "892",
      "DOI": "10.1016/0955-0674(89)90055-0",
      "article-title": "Calcium-dependent cell adhesion molecules.",
      "volume": "1",
      "author": "Kemler",
      "year": "1989",
      "journal-title": "Curr. Opin. Cell Biol"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C11",
      "doi-asserted-by": "crossref",
      "first-page": "149",
      "DOI": "10.1016/S1043-4682(10)80011-X",
      "article-title": "Classical cadherins.",
      "volume": "3",
      "author": "Kemler",
      "year": "1992",
      "journal-title": "Semin. Cell Biol"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C12",
      "doi-asserted-by": "crossref",
      "first-page": "631",
      "DOI": "10.1146/annurev.bi.54.070185.003215",
      "article-title": "Assembly of asparagine-linked oligosaccharides. Ann\".",
      "volume": "54",
      "author": "Kornfeld",
      "year": "1985",
      "journal-title": "Rev. Biochem"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C13",
      "doi-asserted-by": "crossref",
      "first-page": "97",
      "DOI": "10.1016/S0959-437X(05)80348-X",
      "article-title": "Baculoviruses: high level expression in insect cells.",
      "volume": "3",
      "author": "Miller",
      "year": "1993",
      "journal-title": "Curr. Opin. Gen. Dev"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C14",
      "doi-asserted-by": "crossref",
      "first-page": "341",
      "DOI": "10.1038/329341a0",
      "article-title": "Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA.",
      "volume": "329",
      "author": "Nagafuchi",
      "year": "1987",
      "journal-title": "Nature"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C15",
      "first-page": "3",
      "article-title": "Teratocareinome de la souris: Differentiation en culture d\u2019un lignee de cellules primitives a potentialit\u00e9s multiple.",
      "volume": "126 A",
      "author": "Nicholas",
      "year": "1975",
      "journal-title": "Ann. Microbiol. (Inst. Pasteur)"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C16",
      "doi-asserted-by": "crossref",
      "first-page": "147",
      "DOI": "10.1016/0092-8674(90)90222-Z",
      "article-title": "Localization of specifity determining sites in cadherin cell adhesion molecules.",
      "volume": "61",
      "author": "Nose",
      "year": "1990",
      "journal-title": "Cell"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C17",
      "doi-asserted-by": "crossref",
      "first-page": "1711",
      "DOI": "10.1002/j.1460-2075.1989.tb03563.x",
      "article-title": "The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species.",
      "volume": "8",
      "author": "Ozawa",
      "year": "1989",
      "journal-title": "EMBO J"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C18",
      "doi-asserted-by": "crossref",
      "first-page": "1033",
      "DOI": "10.1016/0092-8674(90)90506-A",
      "article-title": "Single amino acid substitutions in one Ca2+ binding site of uvomorulin abolish the adhesive function.",
      "volume": "63",
      "author": "Ozawa",
      "year": "1990",
      "journal-title": "Cell"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C19",
      "doi-asserted-by": "crossref",
      "first-page": "1645",
      "DOI": "10.1083/jcb.111.4.1645",
      "article-title": "Correct proteolytic cleavage is required for the cell adhesive function of uvomorulin.",
      "volume": "Ill",
      "author": "Ozawa",
      "year": "1990",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C20",
      "first-page": "49",
      "article-title": "A possible new adhesive site in the cell-adhesion molecule uvomorulin.",
      "volume": "33",
      "author": "Ozawa",
      "year": "1991",
      "journal-title": "Meeh. Dev"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C21",
      "doi-asserted-by": "crossref",
      "first-page": "451",
      "DOI": "10.1093/nar/17.1.451",
      "article-title": "An efficient procedure for the isolation of recombinant baculovirus.",
      "volume": "17",
      "author": "Pen",
      "year": "1989",
      "journal-title": "Nucl. Acids Res"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C22",
      "doi-asserted-by": "crossref",
      "first-page": "3647",
      "DOI": "10.1002/j.1460-2075.1987.tb02697.x",
      "article-title": "The structure of cell adhesion molecule uvomorulin. Insights into the molecular mechanism of Ca2+-dependent cell adhesion.",
      "volume": "6",
      "author": "Ringwald",
      "year": "1987",
      "journal-title": "EMBO J"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C23",
      "volume-title": "Molecular Cloning - A Laboratory Manual, 2nd edn",
      "author": "Sambrook",
      "year": "1989"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C24",
      "doi-asserted-by": "crossref",
      "first-page": "245",
      "DOI": "10.1247/csf.11.245",
      "article-title": "N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin.",
      "volume": "11",
      "author": "Shirayoshi",
      "year": "1986",
      "journal-title": "Cell Struct. Fund"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C25",
      "volume-title": "A Manual Of Methods For Baculovirus Vectors And Insect Cell Culture Procedures: Bulletin no. 1555, Texas Agricultural Experimental Station, College Station, TX 77843",
      "author": "Summers",
      "year": "1987"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C26",
      "doi-asserted-by": "crossref",
      "first-page": "639",
      "DOI": "10.1242/dev.102.4.639",
      "article-title": "The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.",
      "volume": "102",
      "author": "Takeichi",
      "year": "1988",
      "journal-title": "Development"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C27",
      "doi-asserted-by": "crossref",
      "first-page": "213",
      "DOI": "10.1007/BF02615077",
      "article-title": "The establishment of two cell lines from the insect Spodoptera frugiperda (lepidoptera: noctuidae).",
      "volume": "13",
      "author": "Vaughn",
      "year": "1977",
      "journal-title": "In Vitro"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C28",
      "doi-asserted-by": "crossref",
      "first-page": "169",
      "DOI": "10.1016/0014-4827(84)90241-6",
      "article-title": "Rabbit antiserum against a purified surface glycoprotein decompacts mouse preimplantation embryos and reacts with specific adult tissues.",
      "volume": "152",
      "author": "Vestweber",
      "year": "1984",
      "journal-title": "Exp. Cell Res"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C29",
      "doi-asserted-by": "crossref",
      "first-page": "269273",
      "DOI": "10.1016/0045-6039(84)90084-8",
      "article-title": "Some structural and functional aspects of the cell adhesion molecule uvomorulin.",
      "volume": "15",
      "author": "Vestweber",
      "year": "1984",
      "journal-title": "Cell Differ"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C30",
      "doi-asserted-by": "crossref",
      "first-page": "3393",
      "DOI": "10.1002/j.1460-2075.1985.tb04095.x",
      "article-title": "Identification of a putative cell adhesion domain of uvomorulin.",
      "volume": "4",
      "author": "Vestweber",
      "year": "1985",
      "journal-title": "EMBOJ"
    },
    {
      "key": "2024071020074959200_JOCES_1993_S17_147C31",
      "first-page": "187",
      "article-title": "Soluble 80-kd fragment of cell-CAM 120/80 disrupts cell-cell adhesion.",
      "volume-title": "J. Cell Biochem",
      "author": "Wheelock",
      "year": "1987"
    }
  ],
  "container-title": "Journal of Cell Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.biologists.com/jcs/article-pdf/1993/Supplement_17/147/3552820/joces_1993_supplement_17_21.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://journals.biologists.com/jcs/article-pdf/1993/Supplement_17/147/3552820/joces_1993_supplement_17_21.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        7,
        10
      ]
    ],
    "date-time": "2024-07-10T20:08:16Z",
    "timestamp": 1720642096000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.biologists.com/jcs/article/1993/Supplement_17/147/58269/Characterization-of-recombinant-E-cadherin"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1993,
        12,
        1
      ]
    ]
  },
  "references-count": 31,
  "journal-issue": {
    "issue": "Supplement_17",
    "published-print": {
      "date-parts": [
        [
          1993,
          12,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1242/jcs.1993.Supplement_17.21",
  "relation": {},
  "ISSN": [
    "0021-9533",
    "1477-9137"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1993,
        12,
        1
      ]
    ]
  }
}