Vimentin affects localization and activity of sodium-glucose cotransporter SGLT1 in membrane rafts
10.1242/jcs.115.4.713
Crossref journal-article
The Company of Biologists
Journal of Cell Science (237)
Abstract

It has been reported that vimentin, a cytoskeleton filament that is expressed only in mesenchymal cells after birth, is re-expressed in epithelial cells in vivo under pathological conditions and in vitro in primary culture. Whether vimentin re-expression is only a marker of cellular dedifferentiation or is instrumental in the maintenance of cell structure and/or function is a matter of debate. To address this issue, we used renal proximal tubular cells in primary culture from vimentin-null mice (Vim-/-) and from wild-type littermates (Vim+/+). The absence of vimentin did not affect cell morphology, proliferation and activity of hydrolases, but dramatically decreased Na-glucose cotransport activity. This phenotype was associated with a specific reduction of SGLT1 protein in the detergent-resistant membrane microdomains (DRM). In Vim+/+cells, disruption of these microdomains by methyl-β-cyclodextrin decreased SGLT1 protein abundance in DRM, a change that was paralleled by a decrease of Na-glucose transport activity. Importantly, we showed that vimentin is located to DRM, but it disappeared after methyl-β-cyclodextrin treatment. In Vim-/- cells,supplementation of cholesterol with cholesterol-methyl-β-cyclodextrin complexes completely restored Na-glucose transport activity. Interestingly,neither cholesterol content nor cholesterol metabolism changed in Vim-/- cells. Our results are consistent with the view that re-expression of vimentin in epithelial cells could be instrumental to maintain the physical state of rafts and, thus, the function of DRM-associated proteins.

Authors 10
  1. Isabelle Runembert (first)
  2. Guillaume Queffeulou (additional)
  3. Pierre Federici (additional)
  4. François Vrtovsnik (additional)
  5. Emma Colucci-Guyon (additional)
  6. Charles Babinet (additional)
  7. Pascale Briand (additional)
  8. Germain Trugnan (additional)
  9. Gérard Friedlander (additional)
  10. Fabiola Terzi (additional)
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Dates
Type When
Created 4 years, 4 months ago (April 23, 2021, 11:28 p.m.)
Deposited 2 years, 8 months ago (Dec. 25, 2022, 4:48 a.m.)
Indexed 1 year, 1 month ago (June 28, 2024, 3:10 a.m.)
Issued 23 years, 6 months ago (Feb. 15, 2002)
Published 23 years, 6 months ago (Feb. 15, 2002)
Published Print 23 years, 6 months ago (Feb. 15, 2002)
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@article{Runembert_2002, title={Vimentin affects localization and activity of sodium-glucose cotransporter SGLT1 in membrane rafts}, volume={115}, ISSN={0021-9533}, url={http://dx.doi.org/10.1242/jcs.115.4.713}, DOI={10.1242/jcs.115.4.713}, number={4}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Runembert, Isabelle and Queffeulou, Guillaume and Federici, Pierre and Vrtovsnik, François and Colucci-Guyon, Emma and Babinet, Charles and Briand, Pascale and Trugnan, Germain and Friedlander, Gérard and Terzi, Fabiola}, year={2002}, month=feb, pages={713–724} }