DOI: 10.1242/jcs.115.12.2603. Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation

Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation

10.1242/jcs.115.12.2603

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

The plasma membrane of cells is composed of lateral heterogeneities,patches and microdomains. These membrane microdomains or lipid rafts are enriched in glycosphingolipids and cholesterol and have been implicated in cellular processes such as membrane sorting and signal transduction. In this study we investigated the importance of lipid raft formation in the innate immune recognition of bacteria using biochemical and fluorescence imaging techniques. We found that receptor molecules that are implicated in lipopolysaccharide (LPS)-cellular activation, such as CD14, heat shock protein(hsp) 70, 90, Chemokine receptor 4 (CXCR4), growth differentiation factor 5(GDF5) and Toll-like receptor 4 (TLR4), are present in microdomains following LPS stimulation. Lipid raft integrity is essential for LPS-cellular activation, since raft-disrupting drugs, such as nystatin or MCD, inhibit LPS-induced TNF-α secretion. Our results suggest that the entire bacterial recognition system is based around the ligation of CD14 by bacterial components and the recruitment of multiple signalling molecules, such as hsp70, hsp90, CXCR4, GDF5 and TLR4, at the site of CD14-LPS ligation, within the lipid rafts.

Bibliography

Triantafilou, M., Miyake, K., Golenbock, D. T., & Triantafilou, K. (2002). Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation. Journal of Cell Science, 115(12), 2603â2611.

Authors 4

Martha Triantafilou (first)
Kensuke Miyake (additional)
Douglas T. Golenbock (additional)
Kathy Triantafilou (additional)

References 57 Referenced 500

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 1:37 p.m.)
Deposited	1 year ago (Aug. 29, 2024, 6:35 a.m.)
Indexed	1 week, 3 days ago (Aug. 22, 2025, 1:05 a.m.)
Issued	23 years, 2 months ago (June 15, 2002)
Published	23 years, 2 months ago (June 15, 2002)
Published Print	23 years, 2 months ago (June 15, 2002)

Funders 0

None

BibTeX

@article{Triantafilou_2002, title={Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation}, volume={115}, ISSN={0021-9533}, url={http://dx.doi.org/10.1242/jcs.115.12.2603}, DOI={10.1242/jcs.115.12.2603}, number={12}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Triantafilou, Martha and Miyake, Kensuke and Golenbock, Douglas T. and Triantafilou, Kathy}, year={2002}, month=jun, pages={2603–2611} }

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  "abstract": "<jats:p>The plasma membrane of cells is composed of lateral heterogeneities,patches and microdomains. These membrane microdomains or lipid rafts are enriched in glycosphingolipids and cholesterol and have been implicated in cellular processes such as membrane sorting and signal transduction. In this study we investigated the importance of lipid raft formation in the innate immune recognition of bacteria using biochemical and fluorescence imaging techniques. We found that receptor molecules that are implicated in lipopolysaccharide (LPS)-cellular activation, such as CD14, heat shock protein(hsp) 70, 90, Chemokine receptor 4 (CXCR4), growth differentiation factor 5(GDF5) and Toll-like receptor 4 (TLR4), are present in microdomains following LPS stimulation. Lipid raft integrity is essential for LPS-cellular activation, since raft-disrupting drugs, such as nystatin or MCD, inhibit LPS-induced TNF-\u03b1 secretion. Our results suggest that the entire bacterial recognition system is based around the ligation of CD14 by bacterial components and the recruitment of multiple signalling molecules, such as hsp70, hsp90, CXCR4, GDF5 and TLR4, at the site of CD14-LPS ligation, within the lipid rafts.</jats:p>",
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      "unstructured": "Anderson, H. A., Hiltbold, E. M. and Roche, P. A.(2000). Concentration of MHC class II molecules in lipid rafts facilitates antigen presentation. Nature Immunol.1, 156-162.",
      "DOI": "10.1038/77842"
    },
    {
      "key": "2021042517261331100_REF2",
      "doi-asserted-by": "crossref",
      "unstructured": "Arbibe, L., Mira, J. P., Teusch, N., Kline, L., Guha, M.,Mackman, N., Godowski, P. J., Ulevitch, R. J. and Knaus, U. G.(2000). Toll-like receptor 2-mediated NF-kB activation requires a Rac1-dependent pathway. Nature Immunol.1, 533-540.",
      "DOI": "10.1038/82797"
    },
    {
      "key": "2021042517261331100_REF3",
      "doi-asserted-by": "crossref",
      "unstructured": "Brown, D. A. and Rose, J. K. (1992). Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell68, 533-544.",
      "DOI": "10.1016/0092-8674(92)90189-J"
    },
    {
      "key": "2021042517261331100_REF4",
      "doi-asserted-by": "crossref",
      "unstructured": "Byrd, C. A., Bornmann, W., Erdjument-Bromage, H., Tempst, P.,Pavletich, N., Rosen, N., Nathan, C. F. and Ding, A. (1999). Heat shock 90 mediates macrophage activation by Taxol and bacterial lipopolysaccharide. Proc. Natl. Acad. Sci. USA96, 5645-5650.",
      "DOI": "10.1073/pnas.96.10.5645"
    },
    {
      "key": "2021042517261331100_REF5",
      "doi-asserted-by": "crossref",
      "unstructured": "Cheng, P. C., Dykstra, M. L., Mitchell, R. N. and Pierce, S. K. A. (1999). A role for lipid rafts in B cell antigen receptor signalling and antigen targeting. J. Exp. Med.190, 1549-1560.",
      "DOI": "10.1084/jem.190.11.1549"
    },
    {
      "key": "2021042517261331100_REF6",
      "doi-asserted-by": "crossref",
      "unstructured": "Chow, J. C., Young, D. W., Golenbock, D., Christ, W. J. and Gusovsky, F. (1999). Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction. J. Biol. Chem.274, 10689-10692.",
      "DOI": "10.1074/jbc.274.16.10689"
    },
    {
      "key": "2021042517261331100_REF7",
      "doi-asserted-by": "crossref",
      "unstructured": "Damjanovitch, S., Vereb, G., Schaper, A., Jenei, A., Matko, J.,Starink, J. P., Fox, G. Q., Arndt-Jovin, D. J. and Jovin, T. M.(1995). Structural hierarchy in the clustering of HLA class I molecules in the plasma membrane of humna lymphoblastoid cells.Proc. Natl. Acad. Sci. USA92, 1122-1126.",
      "DOI": "10.1073/pnas.92.4.1122"
    },
    {
      "key": "2021042517261331100_REF8",
      "doi-asserted-by": "crossref",
      "unstructured": "Delude, R. L., Yoshimura, A., Ingalls, R. R. and Golenbock, D. T. (1998). Construction of a lipopolysaccharide reporter cell line and its use in identifying mutants defective in endotoxin, but not TNF-a,signal transduction. J. Immunol.161, 3001-3009.",
      "DOI": "10.4049/jimmunol.161.6.3001"
    },
    {
      "key": "2021042517261331100_REF9",
      "unstructured": "Edidin, M. (1996). Getting there is only half the fun. Curr. Top. Membr.43, 8-13."
    },
    {
      "key": "2021042517261331100_REF10",
      "doi-asserted-by": "crossref",
      "unstructured": "Grakoui, A., Bromley, S. K., Sumen, C., Davis, M. M., Shaw, A. S., Allen, P. M. and Dustin, M. L. (1999). The immunological synapse: a molecular machine controlling T-cell activation.Science285, 221-227.",
      "DOI": "10.1126/science.285.5425.221"
    },
    {
      "key": "2021042517261331100_REF11",
      "doi-asserted-by": "crossref",
      "unstructured": "Hajjar, A. M., O'Mahony, D. S., Ozinsky, A., Underhill, D. M.,Aderem, A., Klebanoff, S. J. and Wilson, C. B. (2001). Functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin. J. Immunol.166, 15-19.",
      "DOI": "10.4049/jimmunol.166.1.15"
    },
    {
      "key": "2021042517261331100_REF12",
      "doi-asserted-by": "crossref",
      "unstructured": "Hambleton, J., Weinstein, S. L., Lem, L. and DeFranco, A. L.(1996). Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA93, 2774-2778.",
      "DOI": "10.1073/pnas.93.7.2774"
    },
    {
      "key": "2021042517261331100_REF13",
      "doi-asserted-by": "crossref",
      "unstructured": "Heine, H., Ulmer, A. J., El-Samalouti, V. T., Lentschat, A. and Hamann, L. (2001). Decay-accelerating factor (DAF/CD55) is a functional active element of the LPS receptor complex. J. Endotoxin Res.7, 227-231.",
      "DOI": "10.1177/09680519010070030601"
    },
    {
      "key": "2021042517261331100_REF14",
      "doi-asserted-by": "crossref",
      "unstructured": "Hirschfeld, M., Ma, Y., Weis, J. H., Vogel, S. N. and Weis, J. J. (2000). Repurification of lipopolysaccharide eliminates signalling through both human and murine toll-like receptor 2. J. Immunol.165, 618-622.",
      "DOI": "10.4049/jimmunol.165.2.618"
    },
    {
      "key": "2021042517261331100_REF15",
      "unstructured": "Horejsi, V., Cebecauer, M., Cerny, J., Brdicka, T., Angelisova,P. and Drbal, K. (1998). Signal transduction in leukocytes via GPI-anchored proteins: an experimental artefact or an aspect of immunoreceptor function? Immunol. Lett.63, 63-73."
    },
    {
      "key": "2021042517261331100_REF16",
      "doi-asserted-by": "crossref",
      "unstructured": "Horejsi, V., Drbal, K., Cebecauer, M., Cerny, J., Brdicka, T.,Angelisova, P. and Stockinger, H. (1999). GPI-microdomains: a role in signalling via immunoreceptors. Immunol. Today20, 356-361.",
      "DOI": "10.1016/S0167-5699(99)01489-9"
    },
    {
      "key": "2021042517261331100_REF17",
      "doi-asserted-by": "crossref",
      "unstructured": "Huby, R. D. J., Dearman, R. J. and Kimber, I.(1999). Intracellular phosphotyrosine induction by major histocompatibility complex class II requires co-aggregation with membrane rafts. J. Biol. Chem.274, 22591-22596.",
      "DOI": "10.1074/jbc.274.32.22591"
    },
    {
      "key": "2021042517261331100_REF18",
      "unstructured": "Jacobson, K. and Dietrich, C. (1999). Looking at lipid rafts? Trends Cell Biol.3, 87-91."
    },
    {
      "key": "2021042517261331100_REF19",
      "doi-asserted-by": "crossref",
      "unstructured": "Jacobson, K., Sheets, E. D. and Simson, R.(1995). Revisiting the fluid mosaic model of membranes.Science268, 1441-1442.",
      "DOI": "10.1126/science.7770769"
    },
    {
      "key": "2021042517261331100_REF20",
      "doi-asserted-by": "crossref",
      "unstructured": "Jenei, A., Varga, S., Bene, L., Matyus, L., Bodnar, A., Bacso,Z., Pieri, C., Gaspar, R., Farkas, T. and Damjanovich, S.(1997). HLA class I and II antigens are partially co-clustered in the plasma membrane of human lymphoblastoid cells. Proc. Natl. Acad. Sci. USA94, 7269-7274.",
      "DOI": "10.1073/pnas.94.14.7269"
    },
    {
      "key": "2021042517261331100_REF21",
      "doi-asserted-by": "crossref",
      "unstructured": "Jiang, Q., Akashi, S., Miyake, K. and Petty, H. R.(2000). Lipopolysaccharide induces physical proximity between CD14 and Toll-like Receptor 4 (TLR4) prior to nuclear translocation of NF-kB.J. Immunol.165, 3541-3544.",
      "DOI": "10.4049/jimmunol.165.7.3541"
    },
    {
      "key": "2021042517261331100_REF22",
      "doi-asserted-by": "crossref",
      "unstructured": "Kabouridis, P. S., Magee, A. I. and Ley, S. C.(1997). S-acylation of LCK protein tyrosine kinase is essential for its signalling function in T-lymphocytes. EMBO J.16, 4983-4998.",
      "DOI": "10.1093/emboj/16.16.4983"
    },
    {
      "key": "2021042517261331100_REF23",
      "doi-asserted-by": "crossref",
      "unstructured": "Keller, P. and Simon, K. (1998). Cholesterol is required for surface transport of influenza virus hemagglutinin. J. Cell Biol.140, 1357-1367.",
      "DOI": "10.1083/jcb.140.6.1357"
    },
    {
      "key": "2021042517261331100_REF24",
      "doi-asserted-by": "crossref",
      "unstructured": "Kenworthy, A. K. and Edidin, M. (1998a). Distribution of a glycosylphosphatidylinositol-anchored protein at the apical surface of MDCK cells examined at a resolution of &lt;100 A using imaging fluorescence resonance energy transfer. J. Cell Biol.142, 69-84.",
      "DOI": "10.1083/jcb.142.1.69"
    },
    {
      "key": "2021042517261331100_REF25",
      "doi-asserted-by": "crossref",
      "unstructured": "Kenworthy, A. K. and Edidin, M. (1998b). Imaging fluorescence resonance energy transfer as probe of membrane organisation and molecular associations of GPI-anchored proteins. InMethods in Molecular Biology (ed. M. H. Gelb), pp. 37-49. Totowa, NJ: Humana Press Inc.",
      "DOI": "10.1385/1-59259-264-3:37"
    },
    {
      "key": "2021042517261331100_REF26",
      "doi-asserted-by": "crossref",
      "unstructured": "Kilsdonk, E. P. (1995). Cellular cholesterol efflux mediated by cyclodextrins. J. Biol. Chem.270, 17250-17256.",
      "DOI": "10.1074/jbc.270.29.17250"
    },
    {
      "key": "2021042517261331100_REF27",
      "doi-asserted-by": "crossref",
      "unstructured": "Kusumi, A. and Sako, Y. (1996). Cell surface organisation by the membrane skeleton. Curr. Opin. Cell Biol.8, 566-574.",
      "DOI": "10.1016/S0955-0674(96)80036-6"
    },
    {
      "key": "2021042517261331100_REF28",
      "doi-asserted-by": "crossref",
      "unstructured": "Lien, E., Means, T. K., Heine, H., Yoshimura, A., Kusumoto, S.,Fukase, K., Fenton, M. J., Oikawa, M., Qureshi, N., Monks, B. et al.(2000). Toll-like receptor 4 imparts ligand-specific recognition of bacterial lipopolysaccharide. J. Clin. Invest.105, 497-504.",
      "DOI": "10.1172/JCI8541"
    },
    {
      "key": "2021042517261331100_REF29",
      "doi-asserted-by": "crossref",
      "unstructured": "Manthey, C. L. and Vogel, S. N. (1994). Elimination of trace endotoxin protein from rough chemotype LPS. J. Endotoxin Res.1, 84-90.",
      "DOI": "10.1177/096805199400100202"
    },
    {
      "key": "2021042517261331100_REF30",
      "doi-asserted-by": "crossref",
      "unstructured": "Monks, C. R. F., Freiberg, B. A., Kupfer, H., Sciaky, N. and Kupfer, A. (1998). Three-dimensional segregation of supramolecular activation clusters in T-cells. Nature395, 82-86.",
      "DOI": "10.1038/25764"
    },
    {
      "key": "2021042517261331100_REF31",
      "doi-asserted-by": "crossref",
      "unstructured": "Perera, P. Y., Mayadas, T. N., Takeuchi, O., Akira, S.,Zaks-Zilberman, M., Goyert, S. M. and Vogel, S. N. (2001). CD11b/CD18 acts in concert with CD14 and Toll-like receptor (TLR)4 to elicit full lipopolisaccharide and taxol-inducible gene expression. J. Immunol.66, 574-581.",
      "DOI": "10.4049/jimmunol.166.1.574"
    },
    {
      "key": "2021042517261331100_REF32",
      "doi-asserted-by": "crossref",
      "unstructured": "Pfeiffer, A., Bottcher, A., Orso, E., Kapinsky, M., Nagy, P.,Bodnar, A., Spreitzer, I., Liebisch, G., Drobnik, W., Gempel, K. et al.(2001). Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts. Eur. J. Immunol.31, 3153-3164.",
      "DOI": "10.1002/1521-4141(200111)31:11<3153::AID-IMMU3153>3.0.CO;2-0"
    },
    {
      "key": "2021042517261331100_REF33",
      "doi-asserted-by": "crossref",
      "unstructured": "Poltorac, A., He, X. L., Smirnova, I., Liu, M. Y., VanHuffel,C., Du, X., Birdwell, D., Alejos, E., Silva, M., Galanos, C. et al.(1998). Defective LPS signaling in C3H/Hej and C57BL/10ScCr mice:Mutations in TLR4 gene. Science282, 2085-2088.",
      "DOI": "10.1126/science.282.5396.2085"
    },
    {
      "key": "2021042517261331100_REF34",
      "doi-asserted-by": "crossref",
      "unstructured": "Pralle, A., Keller, P., Florin, E. L., Simons, K. and Horber, J. K. H. (2000). Sphingolipid-cholesterol rafts diffuse as small entities in the plasma membrane of mammalian cells. J. Cell Biol.148, 997-1007.",
      "DOI": "10.1083/jcb.148.5.997"
    },
    {
      "key": "2021042517261331100_REF35",
      "doi-asserted-by": "crossref",
      "unstructured": "Pugin, J., Kravchenko, V. V., Lee, J. D., Kline, L., Ulevitch,R. J. and Tobias, P. S. (1998). Cell activation mediated by glycosylphosphatidylinositol-anchored or transmembrane forms of CD14.Infect. Immun.66, 1175-1180.",
      "DOI": "10.1128/IAI.66.3.1174-1180.1998"
    },
    {
      "key": "2021042517261331100_REF36",
      "doi-asserted-by": "crossref",
      "unstructured": "Rodgers, W. and Rose, J. K. (1996). Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains. J. Cell Biol.135, 1515-1523.",
      "DOI": "10.1083/jcb.135.6.1515"
    },
    {
      "key": "2021042517261331100_REF37",
      "doi-asserted-by": "crossref",
      "unstructured": "Romagnoli, P. and Bron, C. (1997). Phosphatidylinositol-based glycolipid-anchored proteins enhance proximal TCR signaling events. J. Immunol.158, 5760-5764.",
      "DOI": "10.4049/jimmunol.158.12.5757"
    },
    {
      "key": "2021042517261331100_REF38",
      "doi-asserted-by": "crossref",
      "unstructured": "Rothberg, K. G. (1992). Caveolin, a protein component of caveolae membrane coats. Cell68, 673-682.",
      "DOI": "10.1016/0092-8674(92)90143-Z"
    },
    {
      "key": "2021042517261331100_REF39",
      "doi-asserted-by": "crossref",
      "unstructured": "Scheiffele, P., Roth, M. G. and Simons, K.(1997). Interaction of influenza virus haemagglutinin with sphingolipid-cholesterol membrane domains via its transmembrane domain.EMBO J.16, 5501-5508.",
      "DOI": "10.1093/emboj/16.18.5501"
    },
    {
      "key": "2021042517261331100_REF40",
      "doi-asserted-by": "crossref",
      "unstructured": "Schroeder, R., London, E. and Brown, D. (1994). Interactions between saturated acyl chains confer detergent resistance on lipids and glycosylphosphatidylinositol (Gpi)-Anchored proteins \u2014gpi-anchored proteins in liposomes and cells show similar behavior.Proc. Natl. Acad. Sci. USA91, 12130-12134.",
      "DOI": "10.1073/pnas.91.25.12130"
    },
    {
      "key": "2021042517261331100_REF41",
      "doi-asserted-by": "crossref",
      "unstructured": "Schromm, A., Bradenburg, K., Loppnow, H., Moran, A. P., Koch, M. H. J., Rietschel, E. and Seydel, U. (2000). Biological activities of lipopolysaccharides are determined by the shape of their lipid A portion. Eur. J. Biochem.267, 2008-2013.",
      "DOI": "10.1046/j.1432-1327.2000.01204.x"
    },
    {
      "key": "2021042517261331100_REF42",
      "doi-asserted-by": "crossref",
      "unstructured": "Simons, K. and Ikonen, E. (1997). Functional rafts in membranes. Nature387, 569-570.",
      "DOI": "10.1038/42408"
    },
    {
      "key": "2021042517261331100_REF43",
      "doi-asserted-by": "crossref",
      "unstructured": "Singer, S. J. and Nicolson, G. L. (1972). The fluid mosaic model of the structure of cell membranes.Science175, 720-731.",
      "DOI": "10.1126/science.175.4023.720"
    },
    {
      "key": "2021042517261331100_REF44",
      "doi-asserted-by": "crossref",
      "unstructured": "Stryer, L. (1978). Fluorescence energy transfer as a spectroscopic ruler. Annu. Rev. Biochem.47, 819-830.",
      "DOI": "10.1146/annurev.bi.47.070178.004131"
    },
    {
      "key": "2021042517261331100_REF45",
      "doi-asserted-by": "crossref",
      "unstructured": "Swantek, J. L., Cobb, M. H. and Geppert, T. D.(1997). Jun N-terminal kinase/sStress-Activated Protein Kinase(JNK/SAPK) is required for lipopolysaccharide stimulation of tumour necrosis factor alpha (TNF-a) translation: Glucocorticoids inhibit TNF-a translation by blocking JNK/SAPK. Mol. Cell Biol.17, 6274-6282.",
      "DOI": "10.1128/MCB.17.11.6274"
    },
    {
      "key": "2021042517261331100_REF46",
      "doi-asserted-by": "crossref",
      "unstructured": "Szollosi, J., Damjanovich, S., Balazs, M., Nagy, P., Tron, L.,Fulwyler, M. J. and Brodsky, F. M. (1989). Physical association between MHC class I and class II molecules detected on the cell surface by flow cytometric energy transfer. J. Immunol.143, 208-215.",
      "DOI": "10.4049/jimmunol.143.1.208"
    },
    {
      "key": "2021042517261331100_REF47",
      "doi-asserted-by": "crossref",
      "unstructured": "Tapping, R. I., Akashi, S., Miyake, K., Godowski, P. J. and Tobias, P. S. (2000). Toll-like receptor 4, but not toll-like receptor 2, is a signalling receptor for Escherichia and Salmonella Lipopolysaccharides. J. Immunol.165, 5780-5787.",
      "DOI": "10.4049/jimmunol.165.10.5780"
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      "key": "2021042517261331100_REF48",
      "doi-asserted-by": "crossref",
      "unstructured": "Tobias, P. S., Soldau, K. and Ulevitch, R. J.(1986). Isolation of a lipopolysaccharide-binding acute phase reactant from rabbit serum. J. Exp. Med.164, 785-793.",
      "DOI": "10.1084/jem.164.3.777"
    },
    {
      "key": "2021042517261331100_REF49",
      "doi-asserted-by": "crossref",
      "unstructured": "Triantafilou, K., Triantafilou, M. and Dedrick, R. L.(2001a). A CD14-independent LPS receptor cluster.Nature Immunol.4, 338-345.",
      "DOI": "10.1038/86342"
    },
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      "key": "2021042517261331100_REF50",
      "doi-asserted-by": "crossref",
      "unstructured": "Triantafilou, K., Triantafilou, M., Ladha, S., Mackie, A.,Fernandez, N., Dedrick, R. L. and Cherry, R. J. (2001b). Fluorescence recovery after photobleaching reveals that lipopolysaccharide rapidly transfers from CD14 to heat shock proteins 70 and 90 on the cell membrane. J. Cell Sci.114, 2535-2545.",
      "DOI": "10.1242/jcs.114.13.2535"
    },
    {
      "key": "2021042517261331100_REF51",
      "doi-asserted-by": "crossref",
      "unstructured": "Vereb, G., Matko, J., Vamosi, G., Ibrahim, S. M., Magyar, E.,Varga, S., Szollosi, J., Jenei, A., Gaspar, R., Waldmann, T. A. and Damjanovitch, S. (2000). Cholesterol-dependent clustering of IL-2Ra and its colocalisation with HLA and CD48 on T lymphoma cells suggest their functional association with lipid rafts. Proc. Natl. Acad. Sci. USA97, 6013-6018.",
      "DOI": "10.1073/pnas.97.11.6013"
    },
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      "key": "2021042517261331100_REF52",
      "doi-asserted-by": "crossref",
      "unstructured": "Viola, A., Schroeder, S., Sakakibara, Y. and Lanzavecchia,A. (1999). T lymphocyte costimulation mediated by reorganization of membrane microdomains. Science283, 680-682.",
      "DOI": "10.1126/science.283.5402.680"
    },
    {
      "key": "2021042517261331100_REF53",
      "unstructured": "Weinstein, S. L., Gold, M. R. and DeFranco, A. L.(1990). Bacterial lipopolysaccharide stimulates protein tyrosine phosphorylation in macrophages. Proc. Natl. Acad. Sci. USA88, 4148-4152."
    },
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      "key": "2021042517261331100_REF54",
      "doi-asserted-by": "crossref",
      "unstructured": "Wright, S. D., Ramos, R. A., Tobias, P. S., Ulevitch, R. J. and Mathison, J. C. (1990). CD14, a receptor for complexes of lipopolysaccharide (LPS) And LPS binding-protein.Science249, 1431-1433.",
      "DOI": "10.1126/science.1698311"
    },
    {
      "key": "2021042517261331100_REF55",
      "doi-asserted-by": "crossref",
      "unstructured": "Wu, P. and Brand, L. (1994). Resonance energy transfer: methods and applications. Anal. Biochem.218, 1-13.",
      "DOI": "10.1006/abio.1994.1134"
    },
    {
      "key": "2021042517261331100_REF56",
      "doi-asserted-by": "crossref",
      "unstructured": "Xavier, R., Brennan, T., Li, Q. Q., McCormack, C. and Seed,B. (1998). Membrane compartmentation is required for efficient T cell activation. Immunity8, 723-732.",
      "DOI": "10.1016/S1074-7613(00)80577-4"
    },
    {
      "key": "2021042517261331100_REF57",
      "doi-asserted-by": "crossref",
      "unstructured": "Yashiro-Ohtani, Y., Zhou, X. Y., Toyo-Oka, K., Tai, X. G., Park,C. S., Hamaoka, T., Abe, R., Miyake, K. and Fujiwara, H.(2000). Non-CD28 co-stimulatory molecules present in T-cell rafts induced T-cell costimulation by enhancing the association of TCR with rafts.J. Immunol.164, 1251-1259.",
      "DOI": "10.4049/jimmunol.164.3.1251"
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