DOI: 10.1242/jcs.114.8.1447. Initiating DNA synthesis: from recruiting to activating the MCM complex

Initiating DNA synthesis: from recruiting to activating the MCM complex

10.1242/jcs.114.8.1447

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT The exact duplication of a genome once per cell division is required of every proliferating cell. To achieve this goal, eukaryotes adopt a strategy that limits every replication origin to a single initiation event within a narrow window of the cell cycle by temporally separating the assembly of the pre-replication complex (pre-RC) from the initiation of DNA synthesis. A key component of the pre-RC is the hexameric MCM complex, which is also the presumed helicase of the growing forks. An elaborate mechanism recruits the MCM complex to replication origins, and a regulatory chain reaction converts the poised, but inactive, MCM complex into an enzymatically active helicase. A growing list of proteins, including Mcm10 and Cdt1, are involved in the recruitment process. Two protein kinases, the Cdc7-Dbf4 kinase (DDK) and the cyclin-dependent kinase (CDK), trigger a chain reaction that results in the phosphorylation of the MCM complex and finally in the initiation of DNA synthesis. A composite picture from recent studies suggests that DDK is recruited to the pre-RC during G1 phase but must wait until S phase to phosphorylate the MCM complex. CDK is required for the recruitment of Cdc45 and other downstream components of the elongation machinery.

Bibliography

Lei, M., & Tye, B. K. (2001). Initiating DNA synthesis: from recruiting to activating the MCM complex. Journal of Cell Science, 114(8), 1447â1454.

Authors 2

Ming Lei (first)
Bik K. Tye (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 24, 2021, 5:14 a.m.)
Deposited	1 year, 5 months ago (March 20, 2024, 7:57 p.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 8:28 a.m.)
Issued	24 years, 4 months ago (April 15, 2001)
Published	24 years, 4 months ago (April 15, 2001)
Published Online	24 years, 4 months ago (April 15, 2001)
Published Print	24 years, 4 months ago (April 15, 2001)

Funders 0

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BibTeX

@article{Lei_2001, title={Initiating DNA synthesis: from recruiting to activating the MCM complex}, volume={114}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.114.8.1447}, DOI={10.1242/jcs.114.8.1447}, number={8}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Lei, Ming and Tye, Bik K.}, year={2001}, month=apr, pages={1447–1454} }

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