DOI: 10.1242/jcs.114.21.3845. Multiple pathways in the trafficking and assembly of connexin 26, 32 and 43 into gap junction intercellular communication channels

Multiple pathways in the trafficking and assembly of connexin 26, 32 and 43 into gap junction intercellular communication channels

10.1242/jcs.114.21.3845

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

The assembly of gap junctions was investigated in mammalian cells expressing connexin (Cx) 26, 32 and 43 fused to green, yellow or cyan fluorescent proteins (GFP, YFP, CFP). Targeting of Cx32-CFP and 43-GFP to gap junctions and gap junctional communication was inhibited in cells treated with Brefeldin A, a drug that disassembles the Golgi. However gap junctions constructed of Cx26-GFP were only minimally affected by Brefeldin A. Nocodazole, a microtubule disruptor, had little effect on the assembly of Cx43-GFP gap junctions, but perturbed assembly of Cx26-GFP gap junctions. Co-expression of Cx26-YFP and Cx32-CFP in cells treated with Brefeldin A resulted in assembly of gap junctions constructed of Cx26-YFP. Two amino acids that distinguish Cx26 from Cx32 in transmembrane domains were mutated in Cx32 to investigate underlying mechanisms determining trafficking routes to gap junctions. One mutation, Cx32I28L, conferred on it partial Cx26-like trafficking properties as well the post-translational membrane insertion characteristics of Cx26, suggesting that a key determinant regulating trafficking was present in the first transmembrane domain. The results provide a protein trafficking basis for specifying and regulating connexin composition of gap junctions and thus selectivity of intercellular signaling, with Cx32 and 43 trafficking through the secretory pathway and Cx26 also following an alternative pathway.

Bibliography

Martin, P. E. M., Blundell, G., Ahmad, S., Errington, R. J., & Evans, W. H. (2001). Multiple pathways in the trafficking and assembly of connexin 26, 32 and 43 into gap junction intercellular communication channels. Journal of Cell Science, 114(21), 3845â3855.

Authors 5

Patricia E. M. Martin (first)
Geraldine Blundell (additional)
Shoeb Ahmad (additional)
Rachel J. Errington (additional)
W. Howard Evans (additional)

References 62 Referenced 132

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 8:27 p.m.)
Deposited	4 years, 3 months ago (May 2, 2021, 8:04 a.m.)
Indexed	1 month, 1 week ago (July 23, 2025, 8:09 a.m.)
Issued	23 years, 9 months ago (Nov. 1, 2001)
Published	23 years, 9 months ago (Nov. 1, 2001)
Published Print	23 years, 9 months ago (Nov. 1, 2001)

Funders 0

None

BibTeX

@article{Martin_2001, title={Multiple pathways in the trafficking and assembly of connexin 26, 32 and 43 into gap junction intercellular communication channels}, volume={114}, ISSN={0021-9533}, url={http://dx.doi.org/10.1242/jcs.114.21.3845}, DOI={10.1242/jcs.114.21.3845}, number={21}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Martin, Patricia E. M. and Blundell, Geraldine and Ahmad, Shoeb and Errington, Rachel J. and Evans, W. Howard}, year={2001}, month=nov, pages={3845–3855} }

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