DOI: 10.1242/jcs.113.3.377. The cell biology of osteoclast function

10.1242/jcs.113.3.377

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT Osteoclasts are multinucleated cells responsible for bone resorption. They have developed an efficient machinery for dissolving crystalline hydroxyapatite and degrading organic bone matrix rich in collagen fibers. When initiating bone resorption, osteoclasts become polarized, and three distinct membrane domains appear: a ruffled border, a sealing zone and a functional secretory domain. Simultaneously, the cytoskeleton undergoes extensive re-organisation. During this process, the actin cytoskeleton forms an attachment ring at the sealing zone, the membrane domain that anchors the resorbing cell to bone matrix. The ruffled border appears inside the sealing zone, and has several characteristics of late endosomal membrane. Extensive vesicle transport to the ruffled border delivers hydrochloric acid and proteases to an area between the ruffled border and the bone surface called the resorption lacuna. In this extracellular compartment, crystalline hydroxyapatite is dissolved by acid, and a mixture of proteases degrades the organic matrix. The degradation products of collagen and other matrix components are endocytosed, transported through the cell and exocytosed through a functional secretory domain. This transcytotic route allows osteoclasts to remove large amounts of matrix-degradation products without losing their tight attachment to underlying bone. It also facilitates further processing of the degradation products intracellularly during the passage through the cell.

Bibliography

VÃ¤Ã¤nÃ¤nen, H. K., Zhao, H., Mulari, M., & Halleen, J. M. (2000). The cell biology of osteoclast function. Journal of Cell Science, 113(3), 377â381.

Authors 4

H. Kalervo Väänänen (first)
Haibo Zhao (additional)
Mika Mulari (additional)
Jussi M. Halleen (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 23, 2021, 11:18 p.m.)
Deposited	1 year, 1 month ago (July 10, 2024, 11 a.m.)
Indexed	14 minutes ago (Sept. 4, 2025, 4:30 a.m.)
Issued	25 years, 7 months ago (Feb. 1, 2000)
Published	25 years, 7 months ago (Feb. 1, 2000)
Published Online	25 years, 7 months ago (Feb. 1, 2000)
Published Print	25 years, 7 months ago (Feb. 1, 2000)

Funders 0

None

BibTeX

@article{V_n_nen_2000, title={The cell biology of osteoclast function}, volume={113}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.113.3.377}, DOI={10.1242/jcs.113.3.377}, number={3}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Väänänen, H. Kalervo and Zhao, Haibo and Mulari, Mika and Halleen, Jussi M.}, year={2000}, month=feb, pages={377–381} }

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