DOI: 10.1242/jcs.113.24.4421. Association of cortactin with dynamic actin in lamellipodia and on endosomal vesicles

Association of cortactin with dynamic actin in lamellipodia and on endosomal vesicles

10.1242/jcs.113.24.4421

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT We have used fluorescent protein tagging to study the localization and dynamics of the actin-binding protein cortactin in living NIH 3T3 fibroblast cells. Cortactin was localized to active lamellipodia and to small cytoplasmic spots. Time-lapse imaging revealed that these cortactin labeled structures were very dynamic. In the lamellipodia, cortactin labeled structures formed at the leading edge and then moved toward the cell center. Experiments with green fluorescent protein (GFP)-tagged actin showed that cortactin movement was coincident with the actin retrograde flow in the lamellipodia. Cytoplasmic cortactin spots also contained F-actin and were propelled by actin polymerization. Arp3, a component of the arp2/3 complex which is a key regulator of actin polymerization, co-localized with cortactin. Cytoplasmic cortactin-labeled spots were found to be associated with endosomal vesicles. Association was asymmetric and approximately half of the endosomes were associated with cortactin spots. Time-lapse imaging suggested that these cortactin and F-actin-containing spots propelled endosomes. Actin polymerization based propulsion may be a common mechanism for endomembrane trafficking in the same manner as used in the plasma membrane protrusions. As cortactin is known to interact with membrane-associated signaling proteins it could have a role in linking signaling complexes with dynamic actin on endosomes and in lamellipodia. Movies available on-line: http://www.biologists.com/JCS/movies/jcs1893.html

Bibliography

Kaksonen, M., Peng, H. B., & Rauvala, H. (2000). Association of cortactin with dynamic actin in lamellipodia and on endosomal vesicles. Journal of Cell Science, 113(24), 4421â4426.

Authors 3

Marko Kaksonen (first)
H. Benjamin Peng (additional)
Heikki Rauvala (additional)

References 28 Referenced 143

10.1016/S0955-0674(98)80070-7 / Curr. Opin. Cell Biol / Membrane traffic during cell locomotion by Bretscher (1998)
10.1128/MCB.18.10.5838 / Mol. Cell Biol / Identification of a novel cortactin SH3 domain-binding protein and its localization to growth cones of cultured neurons by Du (1998)
10.1242/jcs.109.2.457 / J. Cell Sci / Actin filaments facilitate two steps of endocytosis by Durrbach (1996)
10.1016/S0955-0674(99)80062-3 / Curr. Opin. Cell Biol / Protein modules as organizers of membrane structure by Fanning (1999)
10.1002/cm.970110403 / Cell Motil. Cytoskel / Centripetal transport of cytoplasm, actin, and the cell surface in lamellipodia of fibroblasts by Fisher (1988)
10.1074/jbc.274.48.34350 / J. Biol. Chem / Internalized epidermal growth factor receptors participate in the activation of p21(ras) in fibroblasts by Haugh (1999)
10.1074/jbc.272.21.13911 / J. Biol. Chem / Down-regulation of the filamentous actin cross-linking activity of cortactin by Src-mediated tyrosine phosphorylation by Huang (1997)
10.1074/jbc.273.40.25770 / J. Biol. Chem / The role of tyrosine phosphorylation of cortactin in the locomotion of endothelial cells by Huang (1998)
10.1074/jbc.273.45.29672 / J. Biol. Chem / Cortactin associates with the cell-cell junction protein ZO-1 in both Drosophila and mouse by Katsube (1998)
10.1074/jbc.273.17.10702 / J. Biol. Chem / Cortactin-Src kinase signaling pathway is involved in N-syndecan-dependent neurite outgrowth by Kinnunen (1998)
10.1074/jbc.272.33.20332 / J. Biol. Chem / The actin cytoskeleton is required for receptor-mediated endocytosis in mammalian cells by Lamaze (1997)
10.1016/0896-6273(95)90220-1 / Neuron / Growth cone advance is inversely proportional to retrograde F-actin flow by Lin (1995)
10.1016/S0896-6273(00)80097-5 / Neuron / Myosin drives retrograde F-actin flow in neuronal growth cones by Lin (1996)
10.1016/S0955-0674(99)80014-3 / Curr. Opin. Cell Biol / The Arp2/3 complex: a multifunctional actin organizer by Machesky (1999)
10.1038/9048 / Nature Cell Biol / Endocytic vesicles move at the tips of actin tails in cultured mast cells by Merrifield (1999)
10.1016/S0092-8674(00)81281-7 / Cell / Actin-based cell motility and cell locomotion by Mitchison (1996)
10.1016/S0896-6273(00)80809-0 / Neuron / Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin by Naisbitt (1999)
10.1242/jcs.100.3.491 / J. Cell Sci / Epidermal growth factor induces rapid reorganization of the actin microfilament system in human A431 cells by Rijken (1991)
10.1016/S0092-8674(00)80988-5 / Cell / Membrane dynamics in endocytosis by Robinson (1996)
10.1016/S0960-9822(00)00384-5 / Curr. Biol / Phosphatidylinositol 4, 5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3 by Rozelle (2000)
10.1083/jcb.143.7.1919 / J. Cell Biol / Visualization and molecular analysis of actin assembly in living cells by Schafer (1998)
10.1083/jcb.141.1.227 / J. Cell Biol / The Ig superfamily cell adhesion molecule, apCAM, mediates growth cone steering by substrate-cytoskeletal coupling by Suter (1998)
10.1083/jcb.148.3.519 / J. Cell Biol / Actin-dependent propulsion of endosomes and lysosomes by recruitment of N-WASP by Taunton (2000)
10.1038/352126a0 / Nature / Actin microfilament dynamics in locomoting cells by Theriot (1991)
10.1083/jcb.119.2.367 / J. Cell Biol / Comparison of actin and cell surface dynamics in motile fibroblasts by Theriot (1992)
10.1038/70281 / Nature Cell Biol / Cooperative symmetry-breaking by actin polymerization in a model for cell motility by van Oudenaarden (1999)
10.1083/jcb.101.2.597 / J. Cell Biol / Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling by Wang (1985)
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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 2:29 p.m.)
Deposited	1 year, 10 months ago (Oct. 18, 2023, 7:07 p.m.)
Indexed	1 month ago (July 22, 2025, 6:35 a.m.)
Issued	24 years, 8 months ago (Dec. 15, 2000)
Published	24 years, 8 months ago (Dec. 15, 2000)
Published Online	24 years, 8 months ago (Dec. 15, 2000)
Published Print	24 years, 8 months ago (Dec. 15, 2000)

Funders 0

None

BibTeX

@article{Kaksonen_2000, title={Association of cortactin with dynamic actin in lamellipodia and on endosomal vesicles}, volume={113}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.113.24.4421}, DOI={10.1242/jcs.113.24.4421}, number={24}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Kaksonen, Marko and Peng, H. Benjamin and Rauvala, Heikki}, year={2000}, month=dec, pages={4421–4426} }

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    "published-print": {
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          15
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  "URL": "http://dx.doi.org/10.1242/jcs.113.24.4421",
  "relation": {},
  "ISSN": [
    "0021-9533",
    "1477-9137"
  ],
  "subject": [],
  "published": {
    "date-parts": [
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        2000,
        12,
        15
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}