DOI: 10.1242/jcs.113.21.3761. Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1

Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1

10.1242/jcs.113.21.3761

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT NIPP1 is a nuclear subunit of protein phosphatase-1 (PP1) that colocalizes with pre-mRNA splicing factors in speckles. We report here that the nuclear and subnuclear targeting of NIPP1, when expressed in HeLa cells or COS-1 cells as a fusion protein with the enhanced-green-fluorescent protein (EGFP), are mediated by distinct sequences. While NIPP1-EGFP can cross the nuclear membrane passively, the active transport to the nucleus is mediated by two independent nuclear localization signals in the central domain of NIPP1, which partially overlap with binding site(s) for PP1. Furthermore, the concentration of NIPP1-EGFP in the nuclear speckles requires the ‘ForkHead-Associated’ domain in the N terminus. This domain is also required for the nuclear retention of NIPP1 when active transport is blocked. Our data imply that the nuclear and subnuclear targeting of NIPP1 are controlled independently.

Bibliography

Jagiello, I., Eynde, A. V., Vulsteke, V., Beullens, M., Boudrez, A., Keppens, S., Stalmans, W., & Bollen, M. (2000). Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1. Journal of Cell Science, 113(21), 3761â3768.

Authors 8

Izabela Jagiello (first)
Aleyde Van Eynde (additional)
Veerle Vulsteke (additional)
Monique Beullens (additional)
An Boudrez (additional)
Stefaan Keppens (additional)
Willy Stalmans (additional)
Mathieu Bollen (additional)

References 22 Referenced 43

10.1146/annurev.biophys.27.1.133 / Annu. Rev. Biophys. Biomol. Struct / The structure and mechanism of protein phosphatases: insights into catalysis and regulation by Barford (1998)
10.1074/jbc.274.20.14053 / J. Biol. Chem / Molecular determinants of nuclear protein phosphatase-1 regulation by NIPP-1 by Beullens (1999)
10.3109/10409239209082564 / Crit. Rev. Biochem. Mol. Biol / The structure, role and regulation of type 1 protein phosphatases by Bollen (1992)
10.1074/jbc.M001676200 / J. Biol. Chem / NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry by Boudrez (2000)
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10.1074/jbc.271.45.28478 / J. Biol. Chem / Identification of protein phosphatase-1-binding proteins by microcystin-biotin affinity chromatography by Campos (1996)
10.1074/jbc.272.21.13823 / J. Biol. Chem / ARD-1 cDNA from human cells encodes a site-specific single-strand endoribonuclease that functionally resembles Escherichia coli RNase E by Claverie-Martin (1997)
10.1016/S0021-9258(19)84673-2 / J. Biol. Chem / Nuclear and nucleolar targeting sequences of c-erb-A, c-myb, N-myc, p53, HSP70, and HIV tat proteins by Dang (1989)
10.1016/S1097-2765(00)80340-8 / Mol. Cell / The FHA domain is a modular phosphopeptide recognition motif by Durocher (1999)
10.1093/emboj/16.8.1876 / EMBO J / Structural basis for the recognition of regulatory subunits by the catalytic subunit of protein phosphatase 1 by Egloff (1997)
10.1146/annurev.cellbio.15.1.607 / Annu. Rev. Cell Dev. Biol / Transport between the cell nucleus and the cytoplasm by Görlich (1999)
10.1074/jbc.270.29.17257 / J. Biol. Chem / Subunit structure and regulation of protein phosphatase-1 in rat liver nuclei by Jagiello (1995)
10.1074/jbc.272.35.22067 / J. Biol. Chem / NIPP-1, a nuclear inhibitory subunit of protein phosphatase-1, has RNA-binding properties by Jagiello (1997)
10.1042/bj3420013 / Biochem. J / Mapping of the RNA-binding and endoribonuclease domains of NIPP1, a nuclear targeting subunit of protein phosphatase 1 by Jin (1999)
10.1006/jmbi.1999.3313 / J. Mol. Biol / Structure and function of a new phosphopeptide-binding domain containing the FHA2 of Rad53 by Liao (1999)
10.1016/S0962-8924(96)20043-1 / Trends Cell Biol / Protein phosphorylation and the nuclear organization of pre-mRNA splicing by Misteli (1997)
10.1146/annurev.cb.10.110194.000415 / Annu. Rev. Cell Biol / Protein serine/threonine phosphatases – new avenues for cell regulation by Shenolikar (1994)
10.1083/jcb.135.2.329 / J. Cell Biol / In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation by Shulga (1996)
10.1242/jcs.112.2.157 / J. Cell Sci / Nuclear organisation of NIPP1, a regulatory subunit of protein phosphatase 1 that associates with pre-mRNA splicing factors by Trinkle-Mulcahy (1999)
10.1074/jbc.270.47.28068 / J. Biol. Chem / Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing by Van Eynde (1995)
10.1074/jbc.272.52.32972 / J. Biol. Chem / Properties and phosphorylation sites of baculovirus-expressed nuclear inhibitor of protein phosphatase-1 (NIPP-1) by Vulsteke (1997)
10.1111/j.1432-1033.1997.00411.x / Eur. J. Biochem / Inhibition of translation by mRNA encoding NIPP-1, a nuclear inhibitor of protein phosphatase-1 by Wera (1997)

Dates

Type	When
Created	4 years, 4 months ago (April 23, 2021, 11:22 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 7:11 p.m.)
Indexed	1 year ago (Aug. 3, 2024, 5:43 p.m.)
Issued	24 years, 10 months ago (Nov. 1, 2000)
Published	24 years, 10 months ago (Nov. 1, 2000)
Published Online	24 years, 10 months ago (Nov. 1, 2000)
Published Print	24 years, 10 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Jagiello_2000, title={Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1}, volume={113}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.113.21.3761}, DOI={10.1242/jcs.113.21.3761}, number={21}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Jagiello, Izabela and Eynde, Aleyde Van and Vulsteke, Veerle and Beullens, Monique and Boudrez, An and Keppens, Stefaan and Stalmans, Willy and Bollen, Mathieu}, year={2000}, month=nov, pages={3761–3768} }

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