DOI: 10.1242/jcs.110.6.687. The E-selectin-ligand ESL-1 is located in the Golgi as well as on microvilli on the cell surface

The E-selectin-ligand ESL-1 is located in the Golgi as well as on microvilli on the cell surface

10.1242/jcs.110.6.687

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

Neutrophils and subsets of lymphocytes bind to E-selectin, a cytokine inducible adhesion molecule on endothelial cells. The E-selectin-ligand-1 (ESL-1) is a high affinity glycoprotein ligand which participates in the binding of mouse myeloid cells to E-selectin. The sequence of mouse ESL-1 is highly homologous to the cysteine rich FGF receptor (CFR) in chicken and the rat Golgi protein MG160. We have analysed the subcellular distribution of ESL-1 by indirect immunofluorescence, flow cytometry, various biochemical techniques and by immunogold scanning electron microscopy. We could localize ESL-1 in the Golgi as well as on the cell surface of 32Dc13 cells and neutrophils. Cell surface staining was confirmed by cell surface biotinylation and by cell surface immunoprecipitations in which antibodies only had access to surface proteins on intact cells. In addition, ESL-1(high) and ESL-1(low) expressing cells, sorted by flow cytometry, gave rise to high and low immunoprecipitation signals for ESL-1, respectively. Based on immunogold labeling of intact cells, we localized ESL-1 on microvilli of 32Dc13 cells and of the lymphoma cell line K46. Quantitative evaluation determined 80% of the total labeling for ESL-1 on microvilli of K46 cells while 69% of the labeling for the control antigen B220 was found on the planar cell surface. These data indicate that ESL-1 occurs at sites on the leukocyte cell surface which are destined for the initiation of cell contacts to the endothelium.

Bibliography

Steegmaier, M., Borges, E., Berger, J., Schwarz, H., & Vestweber, D. (1997). The E-selectin-ligand ESL-1 is located in the Golgi as well as on microvilli on the cell surface. Journal of Cell Science, 110(6), 687â694.

Authors 5

M. Steegmaier (first)
E. Borges (additional)
J. Berger (additional)
H. Schwarz (additional)
D. Vestweber (additional)

References 39 Referenced 60

10.1002/sca.4950140302 / Scanning / An efficient single crystal BSE detector in SEM. by Autrata (1992)
10.1126/science.7692600 / Science / Binding of L-selectin to the vascular sialomucin CD34. by Baumhueter (1993)
10.1016/0092-8674(93)90305-A / Cell / 4 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. by Berlin (1993)
10.1002/eji.1830241215 / Eur. J. Immunol / Only simultaneous blocking of the L-and P-selectin completely inhibits neutrophil migration into mouse peritoneum. by Bosse (1994)
{'key': '2021042516451447800_R5', 'first-page': '27', 'article-title': 'Disparate localization of 55-kd and 75-kd tumor necrosis factor receptors in human endothelial cells.', 'volume': '146', 'author': 'Bradley', 'year': '1995', 'journal-title': 'Am. J. Pathol'} / Am. J. Pathol / Disparate localization of 55-kd and 75-kd tumor necrosis factor receptors in human endothelial cells. by Bradley (1995)
10.1016/0092-8674(91)90279-8 / Cell / Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity. by Butcher (1991)
10.1016/S0021-9258(18)51516-7 / J. Biol. Chem / Isolation of a receptor for acidic and basic fibroblast growth factor from embryonic chick. by Burrus (1989)
10.1128/MCB.12.12.5600 / Mol. Cell. Biol / Identification of a cysteine-rich receptor for fibroblast growth factors. by Burrus (1992)
10.1177/41.3.7679125 / J. Histochem. Cytochem / Detection and spatial distribution of the2 integrin (Mac-1) and L-selectin (LECAM-1) adherence receptors on human neutrophils by high-resolution field emission SEM. by Erlandsen (1993)
10.1038/304030a0 / Nature / A cell-surface molecule involved in organ-specific homing of lymphocytes. by Gallatin (1983)
10.1016/S0021-9258(17)31310-8 / J. Biol. Chem / MG-160: a novel sialoglycoprotein of the medial cisternae of the Golgi apparatus. by Gonatas (1989)
10.1242/jcs.108.2.457 / J. Cell Sci / MG160, a membrane sialoglycoprotein of the medial cisternae of the rat Golgi apparatus, binds basic fibroblast growth factor and exhibits a high level of sequence identity to a chicken fibroblast growth factor receptor. by Gonatas (1995)
10.1021/bi00182a011 / Biochemistry / 6-Sulfated sialyl Lewis × is a major capping group of GlyCAM-1. by Hemmerich (1994)
10.1038/361555a0 / Nature / Sulphation requirement for GlyCAM-1, an endothelial ligand for L-selectin. by Imai (1993)
10.1002/j.1460-2075.1994.tb06690.x / EMBO J / Two new proteins preferentially associated with membrane immunoglobulin D. by Kim (1994)
10.1146/annurev.bi.61.070192.001515 / Annu. Rev. Biochem / Structure and function of the mannose 6-phosphate/ insulinlike growth factor II receptors. by Kornfeld (1992)
10.1016/0092-8674(92)90612-G / Cell / An endothelial ligand for L-selectin is a novel mucin-like molecule. by Lasky (1992)
10.1146/annurev.bi.64.070195.000553 / Annu. Rev. Biochem / Selectin-carbohydrate interactions and the initiation of the inflammatory response. by Lasky (1995)
10.1083/jcb.125.2.471 / J. Cell Biol / Monospecific and common glycoprotein ligands for E-and P-selectin on myeloid cells. by Lenter (1994)
10.1083/jcb.121.2.449 / J. Cell Biol / Identification of a glycoprotein ligand for E-selectin on mouse myeloid cells. by Levinovitz (1993)
10.1091/mbc.4.7.679 / Mol. Biol. Cell / Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa. by Linstedt (1993)
10.1083/jcb.128.4.661 / J. Cell Biol / P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin. by Moore (1995)
10.1002/eji.1830231013 / Eur. J. Immunol / Murine platelet endothelial cell adhesion molecule (PECAM-1)/CD31 modulates2 integrins on lymphokine-activated killer cells. by Piala (1993)
10.1073/pnas.85.10.3479 / Proc. Nat. Acad. Sci. USA / Gene sharing by d-crystallin and argininosuccinate lysate. by Piatigorsky (1988)
10.1016/0092-8674(89)90956-2 / Cell / Enzyme/crystallins: gene sharing as an evolutionary strategy. by Piatigorsky (1989)
10.1016/0092-8674(91)90438-5 / Cell / The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140. by Picker (1991)
10.1016/0092-8674(95)90174-4 / Cell / PSGL-1 recognition of P-selectin is controlled by a tyrosine sulfation consensus at the PSGL-1 amino terminus. by Pouyani (1995)
10.1016/0092-8674(93)90327-M / Cell / Expression cloning of a functional glycoprotein ligand for P-selectin. by Sako (1993)
10.1016/0092-8674(95)90173-6 / Cell / A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding. by Sako (1995)
10.1016/0092-8674(94)90337-9 / Cell / Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. by Springer (1994)
10.1038/373615a0 / Nature / The E-selectin-ligand ESL-1 is a variant of a receptor for fibroblast growth factor. by Steegmaier (1995)
10.1006/excr.1995.1265 / Exp. Cell Res / MG160, a membrane protein of the Golgi apparatus which is homologous to a fibroblast growth factor receptor and to a ligand for E-selectin, is found only in the Golgi apparatus and appears early in chicken embryo development. by Stieber (1995)
10.1016/0167-5699(92)90116-O / Immunol. Today / Two TNF receptors. by Tartaglia (1992)
10.1016/S0923-2494(93)80055-4 / Res. Immunol / Glycoprotein ligands of the two endothelial selectins. by Vestweber (1994)
10.1016/0092-8674(95)90278-3 / Cell / A central role for microvillous receptor presentation in leukocyte adhesion under flow. by von Andrian (1995)
10.1016/S0021-9258(18)42162-X / J. Biol. Chem / Cloning of the mouse endothelial selectins: Expression of both E and P-selectin is inducible by tumor necrosis factor-. by Weller (1992)
10.1038/326622a0 / Nature / The enzyme lactate dehyrogenase as a structural protein in avian and crocodilian lenses. by Wistow (1987)
10.1083/jcb.107.6.2729 / J. Cell Biol / t-Crystallin/-enolase: one gene encodes both an enzyme and a lens structural protein. by Wistow (1988)
{'key': '2021042516451447800_R39', 'first-page': '136', 'article-title': 'Characterization of the FGF binding domain of the cysteine-rich FGF receptor.', 'volume': '5', 'author': 'Zhou', 'year': '1994', 'journal-title': 'Mol. Biol. Cell'} / Mol. Biol. Cell / Characterization of the FGF binding domain of the cysteine-rich FGF receptor. by Zhou (1994)

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 12:49 p.m.)
Deposited	4 years, 4 months ago (April 25, 2021, 12:50 p.m.)
Indexed	5 days, 7 hours ago (Aug. 27, 2025, 12:35 p.m.)
Issued	28 years, 5 months ago (March 15, 1997)
Published	28 years, 5 months ago (March 15, 1997)
Published Print	28 years, 5 months ago (March 15, 1997)

Funders 0

None

BibTeX

@article{Steegmaier_1997, title={The E-selectin-ligand ESL-1 is located in the Golgi as well as on microvilli on the cell surface}, volume={110}, ISSN={0021-9533}, url={http://dx.doi.org/10.1242/jcs.110.6.687}, DOI={10.1242/jcs.110.6.687}, number={6}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Steegmaier, M. and Borges, E. and Berger, J. and Schwarz, H. and Vestweber, D.}, year={1997}, month=mar, pages={687–694} }

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      "article-title": "4 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1.",
      "volume": "74",
      "author": "Berlin",
      "year": "1993",
      "journal-title": "Cell"
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      "article-title": "Only simultaneous blocking of the L-and P-selectin completely inhibits neutrophil migration into mouse peritoneum.",
      "volume": "24",
      "author": "Bosse",
      "year": "1994",
      "journal-title": "Eur. J. Immunol"
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    {
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      "first-page": "27",
      "article-title": "Disparate localization of 55-kd and 75-kd tumor necrosis factor receptors in human endothelial cells.",
      "volume": "146",
      "author": "Bradley",
      "year": "1995",
      "journal-title": "Am. J. Pathol"
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      "article-title": "Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity.",
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      "article-title": "Isolation of a receptor for acidic and basic fibroblast growth factor from embryonic chick.",
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      "author": "Burrus",
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      "author": "Gonatas",
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      "author": "Hemmerich",
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      "author": "Kim",
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      "key": "2021042516451447800_R16",
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      "volume": "61",
      "author": "Kornfeld",
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      "author": "Lasky",
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      "volume": "125",
      "author": "Lenter",
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      "volume": "121",
      "author": "Levinovitz",
      "year": "1993",
      "journal-title": "J. Cell Biol"
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      "author": "Moore",
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      "journal-title": "Cell"
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      "volume": "75",
      "author": "Sako",
      "year": "1993",
      "journal-title": "Cell"
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      "author": "Sako",
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      "volume": "76",
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      "author": "Tartaglia",
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      "volume": "144",
      "author": "Vestweber",
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      "volume": "267",
      "author": "Weller",
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      "article-title": "Characterization of the FGF binding domain of the cysteine-rich FGF receptor.",
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