DOI: 10.1242/jcs.110.4.439. Subcellular localization of myosin V in nerve growth cones and outgrowth from <i>dilute-lethal</i> neurons

Subcellular localization of myosin V in nerve growth cones and outgrowth from dilute-lethal neurons

10.1242/jcs.110.4.439

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT Myosin V-null mice (dilute-lethal mutants) exhibit apparent neurological defects that worsen from birth until death in the third postnatal week. Although myosin V is enriched in brain, the neuronal function of myosin V is unclear and the underlying cause of the neurological defects in these mice is unknown. To aide in understanding myosin V function, we examined the distribution of myosin V in the rodent superior cervical ganglion (SCG) growth cone, a well characterized neuronal structure in which myosin V is concentrated. Using affinity purified, myosin V-specific antibodies in immunofluorescence and immunoelectron microscopy, we observed that myosin V is concentrated in organellerich regions of the growth cone. Myosin V is present on a distinct population of small (50-100 nm) organelles, and on actin filaments and the plasma membrane. Myosin V-associated organelles are present on both microtubules and actin filaments. These results indicate that myosin V may be carried as a passenger on organelles that are transported along microtubules, and that these organelles may also be capable of movement along actin filaments. In addition, we found no abnormalities in outgrowth, morphology, or cytoskeletal organization of SCG growth cones from dilute-lethal mice. These results indicate that myosin V is not necessary for the traction force needed for growth cone locomotion, for organization of the actin cytoskeleton, or for filopodial dynamics.

Bibliography

Evans, L. L., Hammer, J., & Bridgman, P. C. (1997). Subcellular localization of myosin V in nerve growth cones and outgrowth from dilute-lethal neurons. Journal of Cell Science, 110(4), 439â449.

Authors 3

L. L. Evans (first)
J. Hammer (additional)
P. C. Bridgman (additional)

References 31 Referenced 90

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{'key': '2024010400055660300_JOCES_110_4_439C3', 'first-page': '39', 'article-title': 'Functional anatomy of the growth cone in relation to its role in locomotion and neurite assembly', 'volume-title': 'In The Nerve Growth Cone', 'author': 'Bridgman', 'year': '1992'} / In The Nerve Growth Cone / Functional anatomy of the growth cone in relation to its role in locomotion and neurite assembly by Bridgman (1992)
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{'key': '2024010400055660300_JOCES_110_4_439C28', 'first-page': '501', 'article-title': 'Molecular analysis of two mouse dilute locus deletion mutations: spontaneous dilute lethal20J and radiation-induced dilute prenatal lethal Aa2 alleles', 'volume': '10', 'author': 'Strobel', 'year': '1990', 'journal-title': 'Mol. Cell Biol'} / Mol. Cell Biol / Molecular analysis of two mouse dilute locus deletion mutations: spontaneous dilute lethal20J and radiation-induced dilute prenatal lethal Aa2 alleles by Strobel (1990)
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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 12:48 p.m.)
Deposited	1 year, 7 months ago (Jan. 3, 2024, 7:06 p.m.)
Indexed	1 month, 3 weeks ago (July 8, 2025, 7:32 a.m.)
Issued	28 years, 6 months ago (Feb. 15, 1997)
Published	28 years, 6 months ago (Feb. 15, 1997)
Published Online	28 years, 6 months ago (Feb. 15, 1997)
Published Print	28 years, 6 months ago (Feb. 15, 1997)

Funders 0

None

BibTeX

@article{Evans_1997, title={Subcellular localization of myosin V in nerve growth cones and outgrowth from dilute-lethal neurons}, volume={110}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.110.4.439}, DOI={10.1242/jcs.110.4.439}, number={4}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Evans, L. L. and Hammer, J. and Bridgman, P. C.}, year={1997}, month=feb, pages={439–449} }

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