Regulation of actin filament dynamics by p38 map kinase-mediated phosphorylation of heat shock protein 27
10.1242/jcs.110.3.357
Crossref journal-article
The Company of Biologists
Journal of Cell Science (237)
Abstract

ABSTRACT We have studied the contribution of the individual kinases of the MAP (mitogen-activated protein) kinase family, including ERK (extracellular-signal regulated kinase), JNK/SAPK (c-JUN NH2-terminal kinase/stress-activated protein kinase) and p38, to activation of the HSP27 (heat shock protein 27) kinase MAPKAP kinase-2/3 and to HSP27 phosphorylation in Chinese hamster CCL39 cells stimulated by either growth factors, cytokines or stressing agents. In vitro assays using fractionated cell extracts or immunoprecipitates indicated that only fractions containing ERK or p38, and not those containing JNK/SAPK, had the capacity to activate MAPKAP kinase-2/3. In vivo, however, it appeared that only p38 is an upstream activator of HSP27 phosphorylation after both stress or growth factor stimulation: expression of an interfering mutant of ras, which blocked the activation of ERK by both types of inducers, had no effect on HSP27 phosphorylation and p38 activation; and the cell-permeant specific inhibitor of p38, SB203580, blocked MAPKAP-kinase2/3 activation and HSP27 phosphorylation. HSP27 has been suggested to have a phosphorylation-activated homeostatic function at the actin cytoskeleton level. This raises the possibility that p38 might be directly involved in mediating actin responses to external stimuli. Accordingly, we observed that a prior activation of p38 increased the stability of the actin microfilaments in cells exposed to cytochalasin D. The effect was dependent on the expression of HSP27 and was totally annihilated by blocking p38 activity with SB203580. The results provide strong support to the idea that activation of p38 during adverse environmental conditions serves a homeostatic function aimed at regulating actin dynamics that would otherwise be destabilized during stress. Its activation during normal agonist stimulation may constitute an additional actin signaling pathway, the importance of which depends on the level of expression of HSP27.

Bibliography

Guay, J., Lambert, H., Gingras-Breton, G., Lavoie, J. N., Huot, J., & Landry, J. (1997). Regulation of actin filament dynamics by p38 map kinase-mediated phosphorylation of heat shock protein 27. Journal of Cell Science, 110(3), 357–368.

Authors 6
  1. Johane Guay (first)
  2. Herman Lambert (additional)
  3. Geneviève Gingras-Breton (additional)
  4. Josée N. Lavoie (additional)
  5. Jacques Huot (additional)
  6. Jacques Landry (additional)
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Dates
Type When
Created 4 years, 4 months ago (April 25, 2021, 12:50 p.m.)
Deposited 1 year, 7 months ago (Jan. 3, 2024, 7:05 p.m.)
Indexed 1 month, 3 weeks ago (July 2, 2025, 2:48 p.m.)
Issued 28 years, 6 months ago (Feb. 1, 1997)
Published 28 years, 6 months ago (Feb. 1, 1997)
Published Online 28 years, 6 months ago (Feb. 1, 1997)
Published Print 28 years, 6 months ago (Feb. 1, 1997)
Funders 0

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@article{Guay_1997, title={Regulation of actin filament dynamics by p38 map kinase-mediated phosphorylation of heat shock protein 27}, volume={110}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.110.3.357}, DOI={10.1242/jcs.110.3.357}, number={3}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Guay, Johane and Lambert, Herman and Gingras-Breton, Geneviève and Lavoie, Josée N. and Huot, Jacques and Landry, Jacques}, year={1997}, month=feb, pages={357–368} }