DOI: 10.1242/jcs.110.22.2855. Absence of orthogonal arrays in kidney, brain and muscle from transgenic knockout mice lacking water channel aquaporin-4

Absence of orthogonal arrays in kidney, brain and muscle from transgenic knockout mice lacking water channel aquaporin-4

10.1242/jcs.110.22.2855

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT Freeze-fracture electron microscopy (FFEM) of kidney collecting duct, muscle, astrocytes in brain, and other mammalian tissues has revealed regular square arrays of intramembrane particles called orthogonal arrays of particles (OAPs). Their possible role in membrane structure and transport have been proposed, and their absence or decrease has been noted in a variety of hereditary and acquired diseases. A transgenic mouse lacking water channel AQP4 was used to show that AQP4 is the OAP protein. FFEM was done on kidney, skeletal muscle, and brain from AQP4 wild-type [+/+], heterozygous [+/−] and knockout [−/−] mice. The [−/−] mice did not express detectable AQP4 protein, but were grossly indistinguish-able from [+/+] mice. FFEM was done on blinded samples of kidney, brain and muscle from 9 mice. In all 6 kidney samples from [+/+] and [+/−] mice, OAPs similar to those in AQP4-transfected CHO cells were found in basolateral membranes of collecting duct principal cells. In all muscle and brain samples from [+/+] and [+/−] mice, OAPs of identical ultrastructure to those in kidney were seen, but in smaller patch sizes. OAPs were not seen in any sample from [−/−] mice. Label-fracture analysis using a peptide-derived AQP4 polyclonal antibody showed immunogold labeling of OAPs in AQP4-expressing CHO cells. These studies provide direct evidence that AQP4 is required for formation of OAPs and is a component of OAPs, thus establishing the identity and function of OAPs.

Bibliography

Verbavatz, J.-M., Ma, T., Gobin, R., & Verkman, A. S. (1997). Absence of orthogonal arrays in kidney, brain and muscle from transgenic knockout mice lacking water channel aquaporin-4. Journal of Cell Science, 110(22), 2855â2860.

Authors 4

Jean-Marc Verbavatz (first)
Tonghui Ma (additional)
Renée Gobin (additional)
A. S. Verkman (additional)

References 36 Referenced 190

{'key': '2024010621445608500_JOCES_110_22_2855C1', 'first-page': '215', 'article-title': 'Localization of the FA-CHIP water channel in frog urinary bladder', 'volume': '73', 'author': 'Abrami', 'year': '1997', 'journal-title': 'Eur. J. Cell Biol'} / Eur. J. Cell Biol / Localization of the FA-CHIP water channel in frog urinary bladder by Abrami (1997)
{'key': '2024010621445608500_JOCES_110_22_2855C2', 'first-page': '637', 'article-title': 'Orthogonal arrays of particles in the plasma membrane of pneumocytes', 'volume': '18', 'author': 'Bartels', 'year': '1986', 'journal-title': 'J. Submicrosc. Cytol'} / J. Submicrosc. Cytol / Orthogonal arrays of particles in the plasma membrane of pneumocytes by Bartels (1986)
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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 12:48 p.m.)
Deposited	1 year, 7 months ago (Jan. 6, 2024, 4:46 p.m.)
Indexed	2 months, 1 week ago (June 18, 2025, 7:26 p.m.)
Issued	27 years, 9 months ago (Nov. 15, 1997)
Published	27 years, 9 months ago (Nov. 15, 1997)
Published Online	27 years, 9 months ago (Nov. 15, 1997)
Published Print	27 years, 9 months ago (Nov. 15, 1997)

Funders 0

None

BibTeX

@article{Verbavatz_1997, title={Absence of orthogonal arrays in kidney, brain and muscle from transgenic knockout mice lacking water channel aquaporin-4}, volume={110}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.110.22.2855}, DOI={10.1242/jcs.110.22.2855}, number={22}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Verbavatz, Jean-Marc and Ma, Tonghui and Gobin, Renée and Verkman, A. S.}, year={1997}, month=nov, pages={2855–2860} }

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