DOI: 10.1242/jcs.110.21.2703. Specific requirements for the ER to Golgi transport of GPI-anchored proteins in yeast

Specific requirements for the ER to Golgi transport of GPI-anchored proteins in yeast

10.1242/jcs.110.21.2703

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT GPI-anchored proteins are attached to the membrane via a glycosylphosphatidylinositol-(GPI) anchor whose carbohydrate core is conserved in all eukaryotes. Apart from membrane attachment, the precise role of the GPI-anchor is not known, but it has been proposed to play a role in protein sorting. We have investigated the transport of the yeast GPI-anchored protein Gas1p. We identified two mutant strains involved in very different cellular processes that are blocked selectively in the transport of GPI-anchored proteins before arrival to the Golgi. The end8-1/ lcb1-100 mutant is defective in ceramide synthesis. In vitro data suggest a requirement for ceramides after the exit from the ER. We therefore propose that ceramides might function in the fusion of a GPI-containing vesicle with the Golgi, but we cannot exclude a role in the ER. The second mutant that blocks the transport of GPI-anchored proteins to the Golgi is ret1-1, a mutant in the α-subunit of coatomer. In both mutants, GPI-anchor attachment is normal and in ret1-1 cells, the GPI-anchors are remodeled with ceramide to the same extent as in wild-type cells.

Bibliography

SÃ¼tterlin, C., Doering, T. L., SchimmÃ¶ller, F., SchrÃ¶der, S., & Riezman, H. (1997). Specific requirements for the ER to Golgi transport of GPI-anchored proteins in yeast. Journal of Cell Science, 110(21), 2703â2714.

Authors 5

Christine Sütterlin (first)
Tamara L. Doering (additional)
Frauke Schimmöller (additional)
Stephan Schröder (additional)
Howard Riezman (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 12:47 p.m.)
Deposited	1 year, 7 months ago (Jan. 6, 2024, 4:45 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 2:48 p.m.)
Issued	27 years, 10 months ago (Nov. 1, 1997)
Published	27 years, 10 months ago (Nov. 1, 1997)
Published Online	27 years, 10 months ago (Nov. 1, 1997)
Published Print	27 years, 10 months ago (Nov. 1, 1997)

Funders 0

None

BibTeX

@article{S_tterlin_1997, title={Specific requirements for the ER to Golgi transport of GPI-anchored proteins in yeast}, volume={110}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.110.21.2703}, DOI={10.1242/jcs.110.21.2703}, number={21}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Sütterlin, Christine and Doering, Tamara L. and Schimmöller, Frauke and Schröder, Stephan and Riezman, Howard}, year={1997}, month=nov, pages={2703–2714} }

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