DOI: 10.1242/jcs.110.15.1741. TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling

TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling

10.1242/jcs.110.15.1741

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT TLS, the product of a gene commonly translocated in liposarcomas (TLS), is prototypical of a newly identified class of nuclear proteins that contain a C-terminal domain with a distinct RNA recognition motif (RRM) surrounded by Arg-Gly-Gly (RGG) repeats. Its unique N terminus serves as an essential transforming domain for a number of fusion oncoproteins in human sarcomas and leukemias. In this study we use an in vivo UV crosslinking procedure to probe the interactions of TLS with RNA. TLS is found to bind RNA in vivo and the association of TLS with RNA is rapidly diminished by treating cells with transcriptional inhibitors. This suggests that the species bound by TLS turns over rapidly. Surprisingly, the RRM was found to be dispensable for RNA binding by TLS in vivo, suggesting that at any one time most of the interactions between TLS and RNA in the cell are not sequence specific. Analysis of inter specific heterokaryons formed between human and mouse or Xenopus cells revealed that TLS engages in rapid nucleocytoplasmic shuttling, a finding confirmed by the ability of anti-TLS antibodies to trap TLS when injected into the cytoplasm of HeLa cells. Cellular fractionation experiments suggest that TLS binds to RNA in both the nucleus and cytoplasm and support the hypothesis that TLS functions as a heterogeneous ribonuclear protein (hnRNP)-like chaperone of RNA. These findings are discussed in the context of the role altered forms of TLS play in cellular transformation.

Bibliography

Zinszner, H., Sok, J., Immanuel, D., Yin, Y., & Ron, D. (1997). TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling. Journal of Cell Science, 110(15), 1741â1750.

Authors 5

Helene Zinszner (first)
John Sok (additional)
David Immanuel (additional)
Yin Yin (additional)
David Ron (additional)

References 32 Referenced 284

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10.1016/0168-9525(89)90100-5 / Trends Genet / How fixed is the differentiated state by Blau (1989)
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10.1126/science.8036511 / Science / Conserved structures and diversity of functions of RNA-binding proteins by Burd (1994)
{'key': '2024010400343399100_JOCES_110_15_1741C6', 'first-page': '724', 'article-title': 'Identification of hnRNP P2 as TLS/FUS using electrospray mass spectrometry', 'volume': '1', 'author': 'Calvio', 'year': '1995', 'journal-title': 'RNA'} / RNA / Identification of hnRNP P2 as TLS/FUS using electrospray mass spectrometry by Calvio (1995)
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10.1038/359162a0 / Nature / Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumors by Delattre (1992)
{'key': '2024010400343399100_JOCES_110_15_1741C9', 'first-page': '415', 'article-title': 'Physical changes in cytoplasmic messenger ribonucleoproteins in cells treated with inhibitors of mRNA transcription', 'volume': '4', 'author': 'Dreyfuss', 'year': '1984', 'journal-title': 'Mol. Cell. Biol'} / Mol. Cell. Biol / Physical changes in cytoplasmic messenger ribonucleoproteins in cells treated with inhibitors of mRNA transcription by Dreyfuss (1984)
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10.1073/pnas.90.12.5752 / Proc. Nat. Acad. Sci. USA / Ewing sarcoma 11;22 translocation produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation by May (1993)
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{'key': '2024010400343399100_JOCES_110_15_1741C19', 'first-page': '3087', 'article-title': 'The EWS gene, involved in Ewing family of tumors, malignant melanoma of soft parts and desmoplastic small round tumors, codes for an RNA binding proteins with novel regulatory domains', 'volume': '9', 'author': 'Ohno', 'year': '1994', 'journal-title': 'Oncogene'} / Oncogene / The EWS gene, involved in Ewing family of tumors, malignant melanoma of soft parts and desmoplastic small round tumors, codes for an RNA binding proteins with novel regulatory domains by Ohno (1994)
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{'key': '2024010400343399100_JOCES_110_15_1741C22', 'first-page': '3717', 'article-title': 'TLS/FUS fusion domain of TLS/FUS-erg chimeric protein resulting from the t(16;21) chromosomal translocation in human myeloid leukemia functions as a transcriptional activation domain', 'volume': '9', 'author': 'Prasad', 'year': '1994', 'journal-title': 'Oncogene'} / Oncogene / TLS/FUS fusion domain of TLS/FUS-erg chimeric protein resulting from the t(16;21) chromosomal translocation in human myeloid leukemia functions as a transcriptional activation domain by Prasad (1994)
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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 12:47 p.m.)
Deposited	1 year, 8 months ago (Jan. 3, 2024, 7:35 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 6, 2025, 8:54 a.m.)
Issued	28 years, 1 month ago (Aug. 1, 1997)
Published	28 years, 1 month ago (Aug. 1, 1997)
Published Online	28 years, 1 month ago (Aug. 1, 1997)
Published Print	28 years, 1 month ago (Aug. 1, 1997)

Funders 0

None

BibTeX

@article{Zinszner_1997, title={TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling}, volume={110}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.110.15.1741}, DOI={10.1242/jcs.110.15.1741}, number={15}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Zinszner, Helene and Sok, John and Immanuel, David and Yin, Yin and Ron, David}, year={1997}, month=aug, pages={1741–1750} }

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