DOI: 10.1242/jcs.109.12.2779. Assembly of ring canals in the male germ line from structural components of the contractile ring

Assembly of ring canals in the male germ line from structural components of the contractile ring

10.1242/jcs.109.12.2779

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT Stable intercellular bridges called ring canals form following incomplete cytokinesis, and interconnect mitotically or meiotically related germ cells. We show that ring canals in Drosophila melanogaster males are surprisingly different from those previously described in females. Mature ring canal walls in males lack actin and appear to derive directly from structural proteins associated with the contractile ring. Ring canal assembly in males, as in females, initiates during cytokinesis with the appearance of a ring of phosphotyrosine epitopes at the site of the contractile ring. Following constriction, actin and myosin II disappear. However, at least four proteins present at the contractile ring remain: the three septins (Pnut, Sep1 and Sep2) and anillin. In sharp contrast, in ovarian ring canals, septins have not been detected, anillin is lost from mature ring canals and filamentous actin is a major component. In both males and females, a highly branched vesicular structure, termed the fusome, interconnects developing germ cells via the ring canals and is thought to coordinate mitotic germ cell divisions. We show that, in males, unlike females, the fusome persists and enlarges following cessation of the mitotic divisions, developing additional branches during meiosis. During differentiation, the fusome and its associated ring canals localize to the distal tip of the elongating spermatids.

Bibliography

Hime, G. R., Brill, J. A., & Fuller, M. T. (1996). Assembly of ring canals in the male germ line from structural components of the contractile ring. Journal of Cell Science, 109(12), 2779â2788.

Authors 3

Gary R. Hime (first)
Julie A. Brill (additional)
Margaret T. Fuller (additional)

References 39 Referenced 230

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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 12:31 p.m.)
Deposited	1 year, 8 months ago (Jan. 3, 2024, 6:59 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 6, 2025, 9 a.m.)
Issued	28 years, 9 months ago (Dec. 1, 1996)
Published	28 years, 9 months ago (Dec. 1, 1996)
Published Online	28 years, 9 months ago (Dec. 1, 1996)
Published Print	28 years, 9 months ago (Dec. 1, 1996)

Funders 0

None

BibTeX

@article{Hime_1996, title={Assembly of ring canals in the male germ line from structural components of the contractile ring}, volume={109}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.109.12.2779}, DOI={10.1242/jcs.109.12.2779}, number={12}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Hime, Gary R. and Brill, Julie A. and Fuller, Margaret T.}, year={1996}, month=dec, pages={2779–2788} }

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