DOI: 10.1242/jcs.105.3.629. Modulation of pp60c-<i>src</i> activity and cellular localization during differentiation of human trophoblast cells in culture

Modulation of pp60c-src activity and cellular localization during differentiation of human trophoblast cells in culture

10.1242/jcs.105.3.629

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT The morphological and functional differentiation of human trophoblast cells ends with the formation of terminally differentiated multinucleated syncytial trophoblasts. This in vivo differentiation is mimicked in vitro during the primary culture of extravillous cytotrophoblasts: isolated mononuclear cytotrophoblasts aggregate and fuse to form syncytia. This in vitro differentiation is associated with an increase in epidermal growth factor receptor (EGF-R) expression and a transitory increase in E-cadherin expression during cell aggregation. In the present study, we investigated the expression of pp60c-src during morphological differentiation of trophoblast cells. Cultures were terminated at various time intervals and pp60c-src was analysed by immunocytochemistry using a specific antibody. In addition, pp60c-src was investigated by western blot analysis and its tyrosine kinase activity was measured concomitantly. In mononuclear cytotrophoblasts, pp60c-src was localized at cell-matrix contacts and during the aggregation of cytotrophoblasts, pp60c-src was distributed on the cell surface at points of cell-cell contact being colocalized with EGF-R and E-cadherin. The kinase activity of the pp60c-src protein increased significantly at day 2 when cells were completely aggregated and started to fuse, and remained elevated while cells underwent further differentiation. Inhibition of pp60c-src by herbimycin A at 0.25 to 1 g/ml during the first day of culture was associated with a decreased expression of tyrosine kinase activity of EGF-R and an increase in E-cadherin expression. These data suggest that pp60c-src is involved in the modulation of trophoblast cell aggregation and fusion leading to syncytial formation.

Bibliography

Rebut-Bonneton, C., Boutemy-Roulier, S., & Evain-Brion, D. (1993). Modulation of pp60c-src activity and cellular localization during differentiation of human trophoblast cells in culture. Journal of Cell Science, 105(3), 629â636.

Authors 3

C. Rebut-Bonneton (first)
S. Boutemy-Roulier (additional)
D. Evain-Brion (additional)

References 28 Referenced 18

10.1002/jcp.1041540115 / J. Cell. Physiol / Increase in epidermal growth factor receptor and its messenger ribonucleic acid levels with differentiation of human trophoblast cells in culture by Alsat (1993)
10.1016/0012-1606(90)90011-7 / Dev. Biol / Aggregation of dispersed human cytotrophoblastic cells: lessons relevant to the morphogenesis of the placenta by Babalola (1990)
10.1073/pnas.82.24.8374 / Proc. Nat. Acad. Sci. USA / Epidermal growth factor regulates its own receptor by Clark (1985)
{'key': '2023122321551748300_JOCES_105_3_629C4', 'first-page': '1137', 'article-title': 'Formation of multinucleated cells in human placental cell in culture', 'volume': '13', 'author': 'Contractor', 'year': '1985', 'journal-title': 'IRCS Med. Sci'} / IRCS Med. Sci / Formation of multinucleated cells in human placental cell in culture by Contractor (1985)
10.1242/dev.113.3.767 / Development / E-cadherin expression during the differentiation of human trophoblasts by Coutifaris (1991)
10.1083/jcb.111.6.3097 / J. Cell Biol / Immunolocalization of the cellular src protein in interphase and mitotic NIH c-src overexpresser cells by David-Pfeuty (1990)
{'key': '2023122321551748300_JOCES_105_3_629C7', 'first-page': '1105', 'article-title': 'Epidermal growth factor-induced stimulation of epidermal growth factor-receptor synthesis in human cytotrophoblasts and A431 carcinoma cells', 'volume': '48', 'author': 'DePalo', 'year': '1988', 'journal-title': 'Cancer Res'} / Cancer Res / Epidermal growth factor-induced stimulation of epidermal growth factor-receptor synthesis in human cytotrophoblasts and A431 carcinoma cells by DePalo (1988)
10.1242/jcs.96.1.131 / J. Cell Sci / Differentiation of human trophoblast cells in vitro as revealed by immunocytochemical staining of desmoplakin and nuclei by Douglas (1990)
{'key': '2023122321551748300_JOCES_105_3_629C9', 'first-page': '2401', 'article-title': 'Blood platelets express high levels on the pp60c-src tyrosine kinase activity', 'volume': '108', 'author': 'Golden', 'year': '1989', 'journal-title': 'Proc. Nat. Acad. Sci. USA'} / Proc. Nat. Acad. Sci. USA / Blood platelets express high levels on the pp60c-src tyrosine kinase activity by Golden (1989)
10.1242/jcs.99.2.207 / J. Cell Sci / Commentary: Cytoskeletal targets for oncogenic tyrosine kinases by Kellie (1991)
10.1210/endo-118-4-1567 / Endocrinology / Purification, characterization, and in vitro differentiation of cytotrophoblast from human term placentae by Kliman (1986)
10.1210/endo-116-3-889 / Endocrinology / Estrogen synthetase activity in human term placental cells in monolayer culture by Lobo (1985)
{'key': '2023122321551748300_JOCES_105_3_629C13', 'first-page': '663', 'article-title': 'Human trophoblast in culture', 'volume-title': 'In Biology of the Trophoblast', 'author': 'Loke', 'year': '1983'} / In Biology of the Trophoblast / Human trophoblast in culture by Loke (1983)
10.1073/pnas.85.14.5001 / Proc. Nat. Acad. Sci. USA / c-src gene product in developing rat brain is enriched in nerve growth cone endings by Maness (1988)
10.1210/jcem-64-4-744 / J. Clin. Endocrinol. Metab / Induction of differentiated trophoblast function by epidermal growth factor. Relation of histochemically detected cellular epidermal growth factor receptor levels by Maruo (1987)
10.1083/jcb.118.3.703 / J. Cell Biol / Cadherin-mediated cell-cell adhesion is perturbed by v-src tyrosine phosphorylation in metastatic fibroblasts by Matsuyoshi (1992)
10.1210/jcem-65-6-1282 / J. Clin.Endocrinol. Metab / Epidermal growth factor induces differentiation and secretion of human chorionic gonadotropin and placental lactogen in normal human placenta by Morrish (1987)
{'key': '2023122321551748300_JOCES_105_3_629C18', 'first-page': '1587', 'article-title': 'Reversal of transformed phenotypes by herbimycin A in src oncogene expressed rat fibroblasts', 'volume': '48', 'author': 'Murakami', 'year': '1988', 'journal-title': 'Cancer Res'} / Cancer Res / Reversal of transformed phenotypes by herbimycin A in src oncogene expressed rat fibroblasts by Murakami (1988)
10.1016/0092-8674(87)90757-4 / Cell / Tyrosine phosphorylation regulates the biochemical and biological properties of pp60c-src by Piwnica-Worms (1987)
10.1210/endo.131.5.1330494 / Endocrinology / Effect of epidermal growth factor on inhibin secretion in human placental cell culture by Qu (1992)
{'key': '2023122321551748300_JOCES_105_3_629C21', 'first-page': '567', 'article-title': 'Localization of the viral and cellular src kinases to perinuclear vesicles in fibroblasts', 'volume': '3', 'author': 'Redmond', 'year': '1992', 'journal-title': 'Cell Growth Differ'} / Cell Growth Differ / Localization of the viral and cellular src kinases to perinuclear vesicles in fibroblasts by Redmond (1992)
10.1083/jcb.100.2.409 / J. Cell Biol / Highly specific antibody to Rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules by Reish (1985)
10.1083/jcb.113.4.867 / J. Cell Biol / Specific proto-oncogenic tyrosine kinases of src family are enriched in cell-to-cell adherens junctions where the level of tyrosine phosphorylation is elevated by Tsukita (1991)
10.1091/mbc.2.2.105 / Cell Reg / Modulation of intercellular adherens-type junction and tyrosine phosphorylation of their components in RSV-transformed cultured chick lens cells by Volberg (1991)
10.1002/j.1460-2075.1992.tb05225.x / EMBO J / The effect of tyrosine-specific protein phosphorylation on the assembly of adherens-junctions by Volberg (1992)
{'key': '2023122321551748300_JOCES_105_3_629C26', 'first-page': '1326', 'article-title': 'Non mitogenic morphoregulatory action of pp60v-src on multicellular epithelial structures', 'volume': '7', 'author': 'Warren', 'year': '1987', 'journal-title': 'Mol. Cell. Biol'} / Mol. Cell. Biol / Non mitogenic morphoregulatory action of pp60v-src on multicellular epithelial structures by Warren (1987)
{'key': '2023122321551748300_JOCES_105_3_629C27', 'first-page': '632', 'article-title': 'Elevated expression of pp60c-src alters a selective morphogenetic property of epithelial cells in vitro without a mitogenic effect', 'volume': '8', 'author': 'Warren', 'year': '1988', 'journal-title': 'Mol. Cell. Biol'} / Mol. Cell. Biol / Elevated expression of pp60c-src alters a selective morphogenetic property of epithelial cells in vitro without a mitogenic effect by Warren (1988)
{'key': '2023122321551748300_JOCES_105_3_629C28', 'first-page': '1254', 'article-title': 'Inhibition of epidermal growth factor receptor biosynthesis caused by the src oncogene product, pp60v-src', 'volume': '10', 'author': 'Wasilenko', 'year': '1990', 'journal-title': 'Mol. Cell. Biol'} / Mol. Cell. Biol / Inhibition of epidermal growth factor receptor biosynthesis caused by the src oncogene product, pp60v-src by Wasilenko (1990)

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 11:33 a.m.)
Deposited	1 year, 8 months ago (Dec. 23, 2023, 4:58 p.m.)
Indexed	4 months, 1 week ago (April 19, 2025, 1:10 a.m.)
Issued	32 years, 1 month ago (July 1, 1993)
Published	32 years, 1 month ago (July 1, 1993)
Published Online	32 years, 1 month ago (July 1, 1993)
Published Print	32 years, 1 month ago (July 1, 1993)

Funders 0

None

BibTeX

@article{Rebut_Bonneton_1993, title={Modulation of pp60c-src activity and cellular localization during differentiation of human trophoblast cells in culture}, volume={105}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.105.3.629}, DOI={10.1242/jcs.105.3.629}, number={3}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Rebut-Bonneton, C. and Boutemy-Roulier, S. and Evain-Brion, D.}, year={1993}, month=jul, pages={629–636} }

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