DOI: 10.1242/jcs.102.4.753. Microinjection of antibodies against talin inhibits the spreading and migration of fibroblasts

Microinjection of antibodies against talin inhibits the spreading and migration of fibroblasts

10.1242/jcs.102.4.753

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT Talin is believed to be one of the key proteins involved in linking actin filaments to extracellular matrix receptors in focal adhesions. Our strategy for studying the function of talin has been to inactivate talin in living fibroblasts in tissue culture through the microinjection of affinity-purified, polyclonal anti-talin antibodies. The effect of the injected anti-talin antibodies on cell spreading was found to depend on how recently the cells had been plated. Cells that were in the process of spreading on a fibronectin substratum, and which had newly developed focal adhesions, were induced to round up and to disassemble many of the adhesions. However, if fibroblasts were allowed to spread completely before they were microinjected with the anti-talin antibody, focal adhesions remained intact and the flat morphology of the cells was unaffected. The percentage of cells that were able to maintain a spread morphology despite the injection of anti-talin antibodies increased during the first few hours after plating on fibronectin substrata. Fibroblasts that were allowed to spread completely before microinjection with the anti-talin antibody retained both intact focal adhesions and a flat, wellspread morphology, but failed to migrate effectively. Our experiments do not directly address the role of talin in mature focal adhesions, but they indicate that talin is essential for the spreading and migration of fibroblasts on fibronectin as well as for the development and initial maintenance of focal adhesions on this substratum.

Bibliography

Nuckolls, G. H., Romer, L. H., & Burridge, K. (1992). Microinjection of antibodies against talin inhibits the spreading and migration of fibroblasts. Journal of Cell Science, 102(4), 753â762.

Authors 3

Glen H. Nuckolls (first)
Lewis H. Romer (additional)
Keith Burridge (additional)

References 33 Referenced 82

10.1016/0014-5793(87)80832-3 / FEBS Lett / Interaction of iodinated vinculin, metavinculin and a^actinin with cytoskeletal proteins by Belkin (1987)
10.1101/SQB.1982.046.01.072 / Cold Spring Harbor Symp. Quant. Biol / Intracellular and Extracellular Components Involved in the Formation of Ventral Surfaces of Fibroblasts by Birchmeler (1982)
10.1083/jcb.97.2.359 / J. Cell Biol / A new protein of adhesion plaques and ruffling membranes by Burridge (1983)
10.1146/annurev.cb.04.110188.002415 / Annu. Rev. Cell Biol / Focal adhesions: Transmembrane junctions between the extracellular matrix and the cytoskeleton by Burridge (1988)
10.1038/308744a0 / Nature / An interaction between vinculin and talin by Burridge (1984)
10.1242/jcs.39.1.149 / J. Cell Sci / The behavior of fibroblasts migrating from chick heart expiants: Changes in adhesion, locomotion and growth, and in the distribution of actomyosin and fibronectin by Couchtnan (1979)
10.1083/jcb.113.6.1351 / J. Cell Biol / Accumulation of talin in nodes at the edge of the lamellipodium and separate incorporation into adhesion plaques of focal contacts in fibroblasts by DePasquale (1991)
10.1083/jcb.107.4.1385 / J. Cell Biol / Fibronectin receptor exhibits high lateral mobility in embryonic locomoting cells but is immobile in focal contacts and fibrillar streaks in stationary cells by Duband (1988)
{'key': '2023100708213721100_JOCES_102_4_753C9', 'first-page': '816', 'volume-title': 'In Methods in Enzymology', 'author': 'Graessmann', 'year': '1980'} / In Methods in Enzymology by Graessmann (1980)
10.1083/jcb.100.6.1948 / J. Cell Biol / Detachment of cells from culture substrate by soluble fibronectin peptides by Hayman (1985)
10.1242/jcs.29.1.197 / J. Cell Sci / Cell to substratum contacts of chick fibroblasts and their relation to the microfilament system. A correlated interference-reflexion and high voltage electronmicroscope study by Heath (1978)
10.1002/cm.970140213 / Cell Motil. Cytoskel / Talin dynamics in living microinjected nonmuscle cells by Hock (1989)
10.1038/320531a0 / Nature / Interactions of plasma membrane fibronectin receptor with talin - a transmembrane linkage by Horwitz (1986)
10.1007/978-1-4684-4673-9_5 / In Monoclonal Antibodies and Functional Cell Lines: Progress and Applications / Adhesion-related integral membrane glycoproteins identified by monoclonal antibodies by Horwitz (1984)
10.1002/cm.970100118 / Cell Motil. Cytoskel / A precursor of the focal contact in cultured fibroblasts by Izzard (1988)
10.1242/jcs.21.1.129 / J. Cell Sci / Cell-to-substrate contacts in living fibroblasts: an interference reflexion study with an evaluation of the technique by Izzard (1976)
10.1242/jcs.42.1.81 / J. Cell Sci / Formation of cell-to-substrate contacts during fibroblast motility: an interference reflection study by Izzard (1980)
10.1002/cm.970030507 / Cell Motil. Cytoskel / Organization and function of structural elements in focal contacts of tissue culture cells by Jockusch (1983)
10.1242/jcs.54.1.23 / J. Cell Sci / Rapid cellular translocation is related to close contacts formed between various cultured cells and their substrata by Kolega (1982)
10.1038/325078a0 / Nature / Blebbing, free Ca++ and mitochondrial membrane potential preceding cell death in hepatocytes by Lemasters (1987)
10.1016/S0021-9258(18)47637-5 / J. Biol. Chem / Purification and characterization of talin from chicken gizzard smooth muscle by Molony (1987)
10.1016/0006-291X(90)90815-5 / Biochem. Biophys. Res. Commun / Direct interaction between talin and actin by Muguruma (1990)
10.1083/jcb.95.2.654 / J. Cell Biol / A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices by Neff (1982)
10.1083/jcb.110.5.1635 / J. Cell Biol / Functional studies of the domains of talin by Nuckolls (1990)
10.1089/hyb.1990.9.57 / Hybridoma / Characterization of monoclonal antibodies to chicken gizzard talin by Otey (1990)
10.1083/jcb.111.2.721 / J. Cell Biol / An interaction between o^actinin and the fh integrin subunit in vitro by Otey (1990)
10.1083/jcb.97.4.1283 / J. Cell Biol / Detection of vinculin-binding proteins with an 1251-vinculin gel overlay technique by Otto (1983)
10.1042/bst0191065 / Biochem. Soc. Trans / osActinin: a direct link between actin and integrins by Pavalko (1991)
10.1242/jcs.92.3.461 / J. Cell Sci / The Relationship Between Talin and Acetylcholine Receptor Clusters in Xenopus Muscle Cells by Rochlin (1989)
10.1016/0006-291X(87)90564-X / Biochem. Biophys. Res. Commun / Specific interaction of vinculin with o^actinin by Wachsstock (1987)
10.1002/j.1460-2075.1990.tb07374.x / EMBO J / Antibody Mapping of Functional Domains in Vinculin by Westmeyer (1990)
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{'key': '2023100708213721100_JOCES_102_4_753C33', 'first-page': '130a', 'article-title': 'Purification of a vinculin binding protein from smooth muscle', 'volume': '105', 'author': 'Wilkins', 'year': '1987', 'journal-title': 'J. Cell Biol'} / J. Cell Biol / Purification of a vinculin binding protein from smooth muscle by Wilkins (1987)

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 6:47 p.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 4:22 a.m.)
Indexed	2 months ago (July 2, 2025, 2:37 p.m.)
Issued	33 years, 1 month ago (Aug. 1, 1992)
Published	33 years, 1 month ago (Aug. 1, 1992)
Published Online	33 years, 1 month ago (Aug. 1, 1992)
Published Print	33 years, 1 month ago (Aug. 1, 1992)

Funders 0

None

BibTeX

@article{Nuckolls_1992, title={Microinjection of antibodies against talin inhibits the spreading and migration of fibroblasts}, volume={102}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.102.4.753}, DOI={10.1242/jcs.102.4.753}, number={4}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Nuckolls, Glen H. and Romer, Lewis H. and Burridge, Keith}, year={1992}, month=aug, pages={753–762} }

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