DOI: 10.1242/jcs.100.2.397. Basket scales of the green alga, <i>Mesostigma viride</i>: Chemistry and ultrastructure

Basket scales of the green alga, Mesostigma viride: Chemistry and ultrastructure

10.1242/jcs.100.2.397

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

ABSTRACT The unicellular green algal flagellate, Mesostigma viride, possesses an extracellular matrix consisting of three layers of highly distinct scales. This study focused upon the elaborate basket scales. The basket scale is approximately 450–500 nm in height and consists of a solid base, two distinct lattices, two upper rims and various struts and roots. Pure preparations of isolated basket scales were obtained and used for subsequent chemical and immunological analyses. X-ray microanalyses revealed the scale as being mineralized with both calcium and phosphorus. Fourier Transform Infrared Spectroscopic analyses revealed the presence of phosphate, suggesting in turn that the basket scale is complexed with calcium phosphate. SDS-gel electrophoresis of partially digested scales revealed the presence of two protein components, a high Mr, non-migrating form and a form with a Mr of approximately 48 000. Further biochemical analyses of scales showed the presence of two major sugars: glucose and the unusual keto sugar acid, 3-deoxy-lyxo-2-heptulosaric acid, or DHA. Antibodies to scales were raised in rats and used in immunolabeling studies. It is estimated from immunofluorescence studies that a cell of 8/cm contains about 800 basket scales. The scales are in a close-packed arrangement resulting in quasi-crystalline arrays upon the cell surface.

Bibliography

Domozych, D. S., Wells, B., & Shaw, P. J. (1991). Basket scales of the green alga, Mesostigma viride: Chemistry and ultrastructure. Journal of Cell Science, 100(2), 397â407.

Authors 3

David S. Domozych (first)
Brian Wells (additional)
Peter J. Shaw (additional)

References 34 Referenced 42

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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 7:26 p.m.)
Deposited	1 year, 9 months ago (Nov. 16, 2023, 7:13 p.m.)
Indexed	1 year, 2 months ago (June 26, 2024, 10:32 a.m.)
Issued	33 years, 10 months ago (Oct. 1, 1991)
Published	33 years, 10 months ago (Oct. 1, 1991)
Published Online	33 years, 10 months ago (Oct. 1, 1991)
Published Print	33 years, 10 months ago (Oct. 1, 1991)

Funders 0

None

BibTeX

@article{Domozych_1991, title={Basket scales of the green alga, Mesostigma viride: Chemistry and ultrastructure}, volume={100}, ISSN={1477-9137}, url={http://dx.doi.org/10.1242/jcs.100.2.397}, DOI={10.1242/jcs.100.2.397}, number={2}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Domozych, David S. and Wells, Brian and Shaw, Peter J.}, year={1991}, month=oct, pages={397–407} }

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