ER-bound PTP1B is targeted to newly forming cell-matrix adhesions
10.1242/jcs.02846
Crossref journal-article
The Company of Biologists
Journal of Cell Science (237)
Abstract

Here, we define the mechanism through which protein tyrosine phosphatase 1B (PTP1B) is targeted to cell-matrix adhesion sites. Green fluorescent protein (GFP)-labeled PTP1B bearing the substrate-trapping mutation D181A was found in punctate structures in lamellae. The puncta co-localized with focal adhesion kinase (FAK) and Src, and defined the distal tips of cell-matrix adhesion sites identified with paxillin and vinculin. PTP1B is largely associated with the external face of the endoplasmic reticulum (ER) and the puncta develop from ER projections over cell-matrix adhesion sites, a process dependent on microtubules. Deletion of the ER-targeting sequence resulted in cytosolic localization and altered the distribution of PTP1B at cell-matrix foci, whereas mutations disrupting interactions with Src homology 3 (SH3) domains, and the insulin and cadherin receptors had no effect. PTP1B recognizes substrates within forming adhesion foci as revealed by its preferential association with paxillin as opposed to zyxin-containing foci. Our results suggest that PTP1B targets to immature cell-matrix foci in newly forming lamellae by dynamic extensions of the ER and contributes to the maturation of these sites.

Bibliography

Hernández, M. V., Sala, M. G. D., Balsamo, J., Lilien, J., & Arregui, C. O. (2006). ER-bound PTP1B is targeted to newly forming cell-matrix adhesions. Journal of Cell Science, 119(7), 1233–1243.

Authors 5
  1. Mariana V. Hernández (first)
  2. Maria G. Davies Sala (additional)
  3. Janne Balsamo (additional)
  4. Jack Lilien (additional)
  5. Carlos O. Arregui (additional)
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Dates
Type When
Created 19 years, 5 months ago (March 7, 2006, 9:32 p.m.)
Deposited 4 years, 1 month ago (July 24, 2021, 1:17 p.m.)
Indexed 2 weeks ago (Aug. 19, 2025, 7:10 a.m.)
Issued 19 years, 5 months ago (April 1, 2006)
Published 19 years, 5 months ago (April 1, 2006)
Published Print 19 years, 5 months ago (April 1, 2006)
Funders 0

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@article{Herna_ndez_2006, title={ER-bound PTP1B is targeted to newly forming cell-matrix adhesions}, volume={119}, ISSN={0021-9533}, url={http://dx.doi.org/10.1242/jcs.02846}, DOI={10.1242/jcs.02846}, number={7}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Hernández, Mariana V. and Sala, Maria G. Davies and Balsamo, Janne and Lilien, Jack and Arregui, Carlos O.}, year={2006}, month=apr, pages={1233–1243} }