DOI: 10.1242/jcs.01583. Ice2p is important for the distribution and structure of the cortical ER network in <i>Saccharomyces cerevisiae</i>

Ice2p is important for the distribution and structure of the cortical ER network in Saccharomyces cerevisiae
10.1242/jcs.01583
Crossref journal-article
The Company of Biologists
Journal of Cell Science (237)
Abstract
In Saccharomyces cerevisiae, the endoplasmic reticulum (ER) is found along the cell periphery (cortical ER) and nucleus (perinuclear ER). In this study, we characterize a novel ER protein called Ice2p that localizes to the cortical and perinuclear ER. Ice2p is predicted to be a type-III transmembrane protein. Cells carrying a genomic disruption of ICE2 display defects in the distribution of cortical ER in mother and daughter cells. Furthermore, fluorescence imaging of ice2Δ cells reveals an abnormal cortical ER tubular network morphology in both the mother cell and the developing bud. Subcellular fractionation analysis using sucrose gradients corroborate the data from the fluorescence studies. Our findings indicate that Ice2p plays a role in forming and/or maintaining the cortical ER network in budding yeast.
Bibliography
de Martin, P. E., Du, Y., Novick, P., & Ferro-Novick, S. (2005). Ice2p is important for the distribution and structure of the cortical ER network in Saccharomyces cerevisiae. Journal of Cell Science, 118(1), 65â77.
Authors 4
Paula Estrada de Martin (first)
Yunrui Du (additional)
Peter Novick (additional)
Susan Ferro-Novick (additional)
References 49 Referenced 44
Dates
Type	When
Created	20 years, 8 months ago (Dec. 7, 2004, 10:23 p.m.)
Deposited	4 years, 4 months ago (April 25, 2021, 3:30 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:32 p.m.)
Issued	20 years, 7 months ago (Jan. 1, 2005)
Published	20 years, 7 months ago (Jan. 1, 2005)
Published Print	20 years, 7 months ago (Jan. 1, 2005)
Funders 0
None
BibTeX
@article{de_Martin_2005, title={Ice2p is important for the distribution and structure of the cortical ER network in Saccharomyces cerevisiae}, volume={118}, ISSN={0021-9533}, url={http://dx.doi.org/10.1242/jcs.01583}, DOI={10.1242/jcs.01583}, number={1}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={de Martin, Paula Estrada and Du, Yunrui and Novick, Peter and Ferro-Novick, Susan}, year={2005}, month=jan, pages={65–77} }
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  "abstract": "<jats:p>In Saccharomyces cerevisiae, the endoplasmic reticulum (ER) is found along the cell periphery (cortical ER) and nucleus (perinuclear ER). In this study, we characterize a novel ER protein called Ice2p that localizes to the cortical and perinuclear ER. Ice2p is predicted to be a type-III transmembrane protein. Cells carrying a genomic disruption of ICE2 display defects in the distribution of cortical ER in mother and daughter cells. Furthermore, fluorescence imaging of ice2\u0394 cells reveals an abnormal cortical ER tubular network morphology in both the mother cell and the developing bud. Subcellular fractionation analysis using sucrose gradients corroborate the data from the fluorescence studies. Our findings indicate that Ice2p plays a role in forming and/or maintaining the cortical ER network in budding yeast.</jats:p>",
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