DOI: 10.1242/jcs.01299. Cytoplasmic dynein regulates the subcellular distribution of mitochondria by controlling the recruitment of the fission factor dynamin-related protein-1

Cytoplasmic dynein regulates the subcellular distribution of mitochondria by controlling the recruitment of the fission factor dynamin-related protein-1

10.1242/jcs.01299

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

While the subcellular organisation of mitochondria is likely to influence many aspects of cell physiology, its molecular control is poorly understood. Here, we have investigated the role of the retrograde motor protein complex, dynein-dynactin, in mitochondrial localisation and morphology. Disruption of dynein function, achieved in HeLa cells either by over-expressing the dynactin subunit, dynamitin (p50), or by microinjection of an anti-dynein intermediate chain antibody, resulted in (a) the redistribution of mitochondria to the nuclear periphery, and (b) the formation of long and highly branched mitochondrial structures. Suggesting that an alteration in the balance between mitochondrial fission and fusion may be involved in both of these changes, overexpression of p50 induced the translocation of the fission factor dynamin-related protein (Drp1) from mitochondrial membranes to the cytosol and microsomes. Moreover, a dominant-negative-acting form of Drp1 mimicked the effects of p50 on mitochondrial morphology, while wild-type Drp1 almost completely restored normal mitochondrial distribution in p50 over-expressing cells. Thus, the dynein/dynactin complex plays an unexpected role in the regulation of mitochondrial morphology in living cells, by controlling the recruitment of Drp1 to these organelles.

Bibliography

Varadi, A., Johnson-Cadwell, L. I., Cirulli, V., Yoon, Y., Allan, V. J., & Rutter, G. A. (2004). Cytoplasmic dynein regulates the subcellular distribution of mitochondria by controlling the recruitment of the fission factor dynamin-related protein-1. Journal of Cell Science, 117(19), 4389â4400.

Authors 6

Aniko Varadi (first)
Linda I. Johnson-Cadwell (additional)
Vincenzo Cirulli (additional)
Yisang Yoon (additional)
Victoria J. Allan (additional)
Guy A. Rutter (additional)

References 52 Referenced 199

Dates

Type	When
Created	21 years ago (Aug. 10, 2004, 9:13 p.m.)
Deposited	4 years, 4 months ago (April 25, 2021, 3:01 p.m.)
Indexed	2 months, 1 week ago (June 17, 2025, 1:26 p.m.)
Issued	20 years, 11 months ago (Sept. 1, 2004)
Published	20 years, 11 months ago (Sept. 1, 2004)
Published Print	20 years, 11 months ago (Sept. 1, 2004)

Funders 0

None

BibTeX

@article{Varadi_2004, title={Cytoplasmic dynein regulates the subcellular distribution of mitochondria by controlling the recruitment of the fission factor dynamin-related protein-1}, volume={117}, ISSN={0021-9533}, url={http://dx.doi.org/10.1242/jcs.01299}, DOI={10.1242/jcs.01299}, number={19}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Varadi, Aniko and Johnson-Cadwell, Linda I. and Cirulli, Vincenzo and Yoon, Yisang and Allan, Victoria J. and Rutter, Guy A.}, year={2004}, month=sep, pages={4389–4400} }

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  "abstract": "<jats:p>While the subcellular organisation of mitochondria is likely to influence many aspects of cell physiology, its molecular control is poorly understood. Here, we have investigated the role of the retrograde motor protein complex, dynein-dynactin, in mitochondrial localisation and morphology. Disruption of dynein function, achieved in HeLa cells either by over-expressing the dynactin subunit, dynamitin (p50), or by microinjection of an anti-dynein intermediate chain antibody, resulted in (a) the redistribution of mitochondria to the nuclear periphery, and (b) the formation of long and highly branched mitochondrial structures. Suggesting that an alteration in the balance between mitochondrial fission and fusion may be involved in both of these changes, overexpression of p50 induced the translocation of the fission factor dynamin-related protein (Drp1) from mitochondrial membranes to the cytosol and microsomes. Moreover, a dominant-negative-acting form of Drp1 mimicked the effects of p50 on mitochondrial morphology, while wild-type Drp1 almost completely restored normal mitochondrial distribution in p50 over-expressing cells. Thus, the dynein/dynactin complex plays an unexpected role in the regulation of mitochondrial morphology in living cells, by controlling the recruitment of Drp1 to these organelles.</jats:p>",
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