DOI: 10.1242/jcs.00759. ER export of ERGIC-53 is controlled by cooperation of targeting determinants in all three of its domains

ER export of ERGIC-53 is controlled by cooperation of targeting determinants in all three of its domains
10.1242/jcs.00759
Crossref journal-article
The Company of Biologists
Journal of Cell Science (237)
Abstract
Selective export of proteins from the endoplasmic reticulum (ER) requires transport signals that have not been fully characterized. Here, we provide the first complete map of ER export determinants of a type I membrane protein, ERGIC-53, that cycles in the early secretory pathway. ER export requires a phenylalanine motif at the C-terminus, known to mediate coat protein II (COPII) interaction, that is assisted by a glutamine in the cytoplasmic domain. Disulfide bond-stabilized oligomerization is also required. Efficient hexamerization depends on the presence of a polar and two aromatic residues in the transmembrane domain (TMD). Oligomerization becomes independent on disulfide bonds when TMD hydrophobicity is increased. ER export is also influenced by TMD length, 21 amino acids being most efficient. When transferred to a signal-less construct, the established targeting motifs reconstitute full transport activity. The results suggest an ER-export mechanism in which transmembrane and luminal determinants mediate oligomerization required for efficient recruitment of ERGIC-53 into budding vesicles via the C-terminal COPII-binding phenylalanine motif.
Bibliography
Nufer, O., Kappeler, F., Guldbrandsen, S., & Hauri, H.-P. (2003). ER export of ERGIC-53 is controlled by cooperation of targeting determinants in all three of its domains. Journal of Cell Science, 116(21), 4429â4440.
Authors 4
Oliver Nufer (first)
Felix Kappeler (additional)
Svend Guldbrandsen (additional)
Hans-Peter Hauri (additional)
References 64 Referenced 90
Dates
Type	When
Created	21 years, 11 months ago (Sept. 22, 2003, 9:26 p.m.)
Deposited	4 years, 2 months ago (June 11, 2021, 7:44 a.m.)
Indexed	2 days, 10 hours ago (Aug. 21, 2025, 1:09 p.m.)
Issued	21 years, 9 months ago (Nov. 1, 2003)
Published	21 years, 9 months ago (Nov. 1, 2003)
Published Print	21 years, 9 months ago (Nov. 1, 2003)
Funders 0
None
BibTeX
@article{Nufer_2003, title={ER export of ERGIC-53 is controlled by cooperation of targeting determinants in all three of its domains}, volume={116}, ISSN={0021-9533}, url={http://dx.doi.org/10.1242/jcs.00759}, DOI={10.1242/jcs.00759}, number={21}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Nufer, Oliver and Kappeler, Felix and Guldbrandsen, Svend and Hauri, Hans-Peter}, year={2003}, month=nov, pages={4429–4440} }
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  "abstract": "<jats:p>Selective export of proteins from the endoplasmic reticulum (ER) requires transport signals that have not been fully characterized. Here, we provide the first complete map of ER export determinants of a type I membrane protein, ERGIC-53, that cycles in the early secretory pathway. ER export requires a phenylalanine motif at the C-terminus, known to mediate coat protein II (COPII) interaction, that is assisted by a glutamine in the cytoplasmic domain. Disulfide bond-stabilized oligomerization is also required. Efficient hexamerization depends on the presence of a polar and two aromatic residues in the transmembrane domain (TMD). Oligomerization becomes independent on disulfide bonds when TMD hydrophobicity is increased. ER export is also influenced by TMD length, 21 amino acids being most efficient. When transferred to a signal-less construct, the established targeting motifs reconstitute full transport activity. The results suggest an ER-export mechanism in which transmembrane and luminal determinants mediate oligomerization required for efficient recruitment of ERGIC-53 into budding vesicles via the C-terminal COPII-binding phenylalanine motif.</jats:p>",
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