DOI: 10.1242/jcs.00339. Cadherin-mediated adhesion is essential for myofibril continuity across the plasma membrane but not for assembly of the contractile apparatus [Penn]

Cadherin-mediated adhesion is essential for myofibril continuity across the plasma membrane but not for assembly of the contractile apparatus

10.1242/jcs.00339

Crossref journal-article

The Company of Biologists

Journal of Cell Science (237)

Abstract

The strong coordinated contraction of heart muscle is dependent on the correct alignment and connection of the myofibrils across the plasma membrane. Previous studies indicate that N-cadherin is involved in cardiac myocyte adhesion and myofibrillogenesis. To investigate whether N-cadherin is specifically required for normal myocyte structure and function, we cultured myocytes from wild-type, N-cadherin-null and mutant embryos expressing the epithelial cadherin E-cadherin. In contrast to previous studies in chicken using N-cadherin-perturbing antibodies, our in vitro studies with mouse cells demonstrate that N-cadherin is not required for myofibrillogenesis, but is critical for myofibril organization. That is, N-cadherin-deficient myocytes beat and myofibrils were well formed; however, alignment of the myofibrils through regions of cell-cell contact was lost, resulting in their random orientation. Gap junctions were perturbed in the N-cadherin-null myocytes. By contrast, focal contacts appeared normal in the mutant cells. Furthermore,E-cadherin restored normal cell morphology and behavior to the N-cadherin-deficient myocytes, including proper alignment of the myofibrils. We conclude that a different adhesive system, most probably integrin, is responsible for myofibrillogenesis in the N-cadherin-null myocytes.

Bibliography

Luo, Y., & Radice, G. L. (2003). Cadherin-mediated adhesion is essential for myofibril continuity across the plasma membrane but not for assembly of the contractile apparatus. Journal of Cell Science, 116(8), 1471â1479.

Authors 2

Yang Luo (first)
Glenn L. Radice (additional)

References 51 Referenced 66

Dates

Type	When
Created	22 years, 5 months ago (March 14, 2003, 12:08 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 12:55 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 7, 2024, 7:53 p.m.)
Issued	22 years, 4 months ago (April 15, 2003)
Published	22 years, 4 months ago (April 15, 2003)
Published Print	22 years, 4 months ago (April 15, 2003)

Funders 0

None

BibTeX

@article{Luo_2003, title={Cadherin-mediated adhesion is essential for myofibril continuity across the plasma membrane but not for assembly of the contractile apparatus}, volume={116}, ISSN={0021-9533}, url={http://dx.doi.org/10.1242/jcs.00339}, DOI={10.1242/jcs.00339}, number={8}, journal={Journal of Cell Science}, publisher={The Company of Biologists}, author={Luo, Yang and Radice, Glenn L.}, year={2003}, month=apr, pages={1471–1479} }

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  "abstract": "<jats:p>The strong coordinated contraction of heart muscle is dependent on the correct alignment and connection of the myofibrils across the plasma membrane. Previous studies indicate that N-cadherin is involved in cardiac myocyte adhesion and myofibrillogenesis. To investigate whether N-cadherin is specifically required for normal myocyte structure and function, we cultured myocytes from wild-type, N-cadherin-null and mutant embryos expressing the epithelial cadherin E-cadherin. In contrast to previous studies in chicken using N-cadherin-perturbing antibodies, our in vitro studies with mouse cells demonstrate that N-cadherin is not required for myofibrillogenesis, but is critical for myofibril organization. That is, N-cadherin-deficient myocytes beat and myofibrils were well formed; however, alignment of the myofibrils through regions of cell-cell contact was lost, resulting in their random orientation. Gap junctions were perturbed in the N-cadherin-null myocytes. By contrast, focal contacts appeared normal in the mutant cells. Furthermore,E-cadherin restored normal cell morphology and behavior to the N-cadherin-deficient myocytes, including proper alignment of the myofibrils. We conclude that a different adhesive system, most probably integrin, is responsible for myofibrillogenesis in the N-cadherin-null myocytes.</jats:p>",
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