DOI: 10.1242/dev.129.22.5161. The limb identity geneTbx5promotes limb initiation by interacting withWnt2bandFgf10

The limb identity geneTbx5promotes limb initiation by interacting withWnt2bandFgf10

10.1242/dev.129.22.5161

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

A major gap in our knowledge of development is how the growth and identity of tissues and organs are linked during embryogenesis. The vertebrate limb is one of the best models to study these processes. Combining mutant analyses with gain- and loss-of-function approaches in zebrafish and chick embryos, we show that Tbx5, in addition to its role governing forelimb identity,is both necessary and sufficient for limb outgrowth. We find thatTbx5 functions downstream of WNT signaling to regulateFgf10, which, in turn, maintains Tbx5 expression during limb outgrowth. Furthermore, our results indicate that Tbx5 andWnt2b function together to initiate and specify forelimb outgrowth and identity. The molecular interactions governed by members of the T-box,Wnt and Fgf gene families uncovered in this study provide a framework for understanding not only limb development, but how outgrowth and identity of other tissues and organs of the embryo may be regulated.

Bibliography

Ng, J. K., Kawakami, Y., BuÌscher, D., Raya, A., Itoh, T., Koth, C. M., Esteban, C. R., RodriÌguez-LeoÌn, J., Garrity, D. M., Fishman, M. C., & Belmonte, J. C. I. (2002). The limb identity geneTbx5promotes limb initiation by interacting withWnt2bandFgf10. Development, 129(22), 5161â5170.

Authors 11

Jennifer K. Ng (first)
Yasuhiko Kawakami (additional)
Dirk Büscher (additional)
Ángel Raya (additional)
Tohru Itoh (additional)
Christopher M. Koth (additional)
Concepción Rodríguez Esteban (additional)
Joaquín Rodríguez-León (additional)
Deborah M. Garrity (additional)
Mark C. Fishman (additional)
Juan Carlos Izpisúa Belmonte (additional)

References 47 Referenced 163

Dates

Type	When
Created	4 years, 3 months ago (April 26, 2021, 10:50 a.m.)
Deposited	2 years, 8 months ago (Dec. 25, 2022, 8:03 a.m.)
Indexed	43 minutes ago (Aug. 25, 2025, 11 p.m.)
Issued	22 years, 9 months ago (Nov. 15, 2002)
Published	22 years, 9 months ago (Nov. 15, 2002)
Published Print	22 years, 9 months ago (Nov. 15, 2002)

Funders 0

None

BibTeX

@article{Ng_2002, title={The limb identity geneTbx5promotes limb initiation by interacting withWnt2bandFgf10}, volume={129}, ISSN={0950-1991}, url={http://dx.doi.org/10.1242/dev.129.22.5161}, DOI={10.1242/dev.129.22.5161}, number={22}, journal={Development}, publisher={The Company of Biologists}, author={Ng, Jennifer K. and Kawakami, Yasuhiko and Büscher, Dirk and Raya, Ángel and Itoh, Tohru and Koth, Christopher M. and Esteban, Concepción Rodríguez and Rodríguez-León, Joaquín and Garrity, Deborah M. and Fishman, Mark C. and Belmonte, Juan Carlos Izpisúa}, year={2002}, month=nov, pages={5161–5170} }

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