DOI: 10.1242/dev.127.20.4481. Lactational competence and involution of the mouse mammary gland require plasminogen

Lactational competence and involution of the mouse mammary gland require plasminogen

10.1242/dev.127.20.4481

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT Urokinase-type plasminogen activator expression is induced in the mouse mammary gland during development and post-lactational involution. We now show that primiparous plasminogen-deficient (Plg−/−) mice have seriously compromised mammary gland development and involution. All mammary glands were underdeveloped and one-quarter of the mice failed to lactate. Although the glands from lactating Plg−/− mice were initially smaller, they failed to involute after weaning, and in most cases they failed to support a second litter. Alveolar regression was markedly reduced and a fibrotic stroma accumulated in Plg−/− mice. Nevertheless, urokinase and matrix metalloproteinases (MMPs) were upregulated normally in involuting glands of Plg−/− mice, and fibrin did not accumulate in the glands. Heterozygous Plg+/− mice exhibited haploinsufficiency, with a definite, but less severe mammary phenotype. These data demonstrate a critical, dose-dependent requirement for Plg in lactational differentiation and mammary gland remodeling during involution.

Bibliography

Lund, L. R., BjÃ¸rn, S. F., Sternlicht, M. D., Nielsen, B. S., Solberg, H., Usher, P. A., Ãsterby, R., Christensen, I. J., Stephens, R. W., Bugge, T. H., DanÃ¸, K., & Werb, Z. (2000). Lactational competence and involution of the mouse mammary gland require plasminogen. Development, 127(20), 4481â4492.

Authors 12

Leif R. Lund (first)
Signe F. Bjørn (additional)
Mark D. Sternlicht (additional)
Boye S. Nielsen (additional)
Helene Solberg (additional)
Pernille A. Usher (additional)
Ruth Østerby (additional)
Ib J. Christensen (additional)
Ross W. Stephens (additional)
Thomas H. Bugge (additional)
Keld Danø (additional)
Zena Werb (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 12:18 a.m.)
Deposited	2 years, 3 months ago (May 11, 2023, 7:24 p.m.)
Indexed	1 week ago (Aug. 19, 2025, 6:24 a.m.)
Issued	24 years, 10 months ago (Oct. 15, 2000)
Published	24 years, 10 months ago (Oct. 15, 2000)
Published Online	24 years, 10 months ago (Oct. 15, 2000)
Published Print	24 years, 10 months ago (Oct. 15, 2000)

Funders 0

None

BibTeX

@article{Lund_2000, title={Lactational competence and involution of the mouse mammary gland require plasminogen}, volume={127}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.127.20.4481}, DOI={10.1242/dev.127.20.4481}, number={20}, journal={Development}, publisher={The Company of Biologists}, author={Lund, Leif R. and Bjørn, Signe F. and Sternlicht, Mark D. and Nielsen, Boye S. and Solberg, Helene and Usher, Pernille A. and Østerby, Ruth and Christensen, Ib J. and Stephens, Ross W. and Bugge, Thomas H. and Danø, Keld and Werb, Zena}, year={2000}, month=oct, pages={4481–4492} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n               <jats:p>Urokinase-type plasminogen activator expression is induced in the mouse mammary gland during development and post-lactational involution. We now show that primiparous plasminogen-deficient (Plg\u2212/\u2212) mice have seriously compromised mammary gland development and involution. All mammary glands were underdeveloped and one-quarter of the mice failed to lactate. Although the glands from lactating Plg\u2212/\u2212 mice were initially smaller, they failed to involute after weaning, and in most cases they failed to support a second litter. Alveolar regression was markedly reduced and a fibrotic stroma accumulated in Plg\u2212/\u2212 mice. Nevertheless, urokinase and matrix metalloproteinases (MMPs) were upregulated normally in involuting glands of Plg\u2212/\u2212 mice, and fibrin did not accumulate in the glands. Heterozygous Plg+/\u2212 mice exhibited haploinsufficiency, with a definite, but less severe mammary phenotype. These data demonstrate a critical, dose-dependent requirement for Plg in lactational differentiation and mammary gland remodeling during involution.</jats:p>",
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