DOI: 10.1242/dev.127.17.3855. A phospholipid kinase regulates actin organization and intercellular bridge formation during germline cytokinesis

A phospholipid kinase regulates actin organization and intercellular bridge formation during germline cytokinesis

10.1242/dev.127.17.3855

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT The endgame of cytokinesis can follow one of two pathways depending on developmental context: resolution into separate cells or formation of a stable intercellular bridge. Here we show that the four wheel drive (fwd) gene of Drosophila melanogaster is required for intercellular bridge formation during cytokinesis in male meiosis. In fwd mutant males, contractile rings form and constrict in dividing spermatocytes, but cleavage furrows are unstable and daughter cells fuse together, producing multinucleate spermatids. fwd is shown to encode a phosphatidylinositol 4-kinase (PI 4-kinase), a member of a family of proteins that perform the first step in the synthesis of the key regulatory membrane phospholipid PIP2. Wild-type activity of the fwd PI 4-kinase is required for tyrosine phosphorylation in the cleavage furrow and for normal organization of actin filaments in the constricting contractile ring. Our results suggest a critical role for PI 4-kinases and phosphatidylinositol derivatives during the final stages of cytokinesis.

Bibliography

Brill, J. A., Hime, G. R., Scharer-Schuksz, M., & Fuller, M. T. (2000). A phospholipid kinase regulates actin organization and intercellular bridge formation during germline cytokinesis. Development, 127(17), 3855â3864.

Authors 4

Julie A. Brill (first)
Gary R. Hime (additional)
Manuela Scharer-Schuksz (additional)
Margaret T. Fuller (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 12:20 a.m.)
Deposited	2 years, 5 months ago (March 28, 2023, 9:20 p.m.)
Indexed	1 month ago (July 26, 2025, 4:59 a.m.)
Issued	25 years ago (Sept. 1, 2000)
Published	25 years ago (Sept. 1, 2000)
Published Online	25 years ago (Sept. 1, 2000)
Published Print	25 years ago (Sept. 1, 2000)

Funders 0

None

BibTeX

@article{Brill_2000, title={A phospholipid kinase regulates actin organization and intercellular bridge formation during germline cytokinesis}, volume={127}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.127.17.3855}, DOI={10.1242/dev.127.17.3855}, number={17}, journal={Development}, publisher={The Company of Biologists}, author={Brill, Julie A. and Hime, Gary R. and Scharer-Schuksz, Manuela and Fuller, Margaret T.}, year={2000}, month=sep, pages={3855–3864} }

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