DOI: 10.1242/dev.125.21.4245. Boundaries in the <i>Drosophila</i> wing imaginal disc organize vein-specific genetic programs

Boundaries in the Drosophila wing imaginal disc organize vein-specific genetic programs

10.1242/dev.125.21.4245

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT Previous studies have suggested that vein primordia in Drosophila form at boundaries along the A/P axis between discrete sectors of the larval wing imaginal disc. Genes involved in initiating vein development during the third larval instar are expressed either in narrow stripes corresponding to vein primordia or in broader ‘provein’ domains consisting of cells competent to become veins. In addition, genes specifying the alternative intervein cell fate are expressed in complementary intervein regions. The regulatory relationships between genes expressed in narrow vein primordia, in broad provein stripes and in interveins remains unknown, however. In this manuscript, we provide additional evidence for veins forming in narrow stripes at borders of A/P sectors. These experiments further suggest that narrow vein primordia produce secondary short-range signal(s), which activate expression of provein genes in a broad pattern in neighboring cells. We also show that crossregulatory interactions among genes expressed in veins, proveins and interveins contribute to establishing the vein-versus-intervein pattern, and that control of gene expression in vein and intervein regions must be considered on a stripe-by-stripe basis. Finally, we present evidence for a second set of vein-inducing boundaries lying between veins, which we refer to as paravein boundaries. We propose that veins develop at both vein and paravein boundaries in more ‘primitive’ insects, which have up to twice the number of veins present in Drosophila. We present a model in which different A/P boundaries organize vein-specific genetic programs to govern the development of individual veins.

Bibliography

Biehs, B., Sturtevant, M. A., & Bier, E. (1998). Boundaries in the Drosophila wing imaginal disc organize vein-specific genetic programs. Development, 125(21), 4245â4257.

Authors 3

Brian Biehs (first)
Mark A. Sturtevant (additional)
Ethan Bier (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 11:26 p.m.)
Deposited	6 months, 2 weeks ago (Feb. 14, 2025, 10:57 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 7, 2025, 4:37 p.m.)
Issued	26 years, 10 months ago (Nov. 1, 1998)
Published	26 years, 10 months ago (Nov. 1, 1998)
Published Online	26 years, 10 months ago (Nov. 1, 1998)
Published Print	26 years, 10 months ago (Nov. 1, 1998)

Funders 0

None

BibTeX

@article{Biehs_1998, title={Boundaries in the Drosophila wing imaginal disc organize vein-specific genetic programs}, volume={125}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.125.21.4245}, DOI={10.1242/dev.125.21.4245}, number={21}, journal={Development}, publisher={The Company of Biologists}, author={Biehs, Brian and Sturtevant, Mark A. and Bier, Ethan}, year={1998}, month=nov, pages={4245–4257} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n               <jats:p>Previous studies have suggested that vein primordia in Drosophila form at boundaries along the A/P axis between discrete sectors of the larval wing imaginal disc. Genes involved in initiating vein development during the third larval instar are expressed either in narrow stripes corresponding to vein primordia or in broader \u2018provein\u2019 domains consisting of cells competent to become veins. In addition, genes specifying the alternative intervein cell fate are expressed in complementary intervein regions. The regulatory relationships between genes expressed in narrow vein primordia, in broad provein stripes and in interveins remains unknown, however. In this manuscript, we provide additional evidence for veins forming in narrow stripes at borders of A/P sectors. These experiments further suggest that narrow vein primordia produce secondary short-range signal(s), which activate expression of provein genes in a broad pattern in neighboring cells. We also show that crossregulatory interactions among genes expressed in veins, proveins and interveins contribute to establishing the vein-versus-intervein pattern, and that control of gene expression in vein and intervein regions must be considered on a stripe-by-stripe basis. Finally, we present evidence for a second set of vein-inducing boundaries lying between veins, which we refer to as paravein boundaries. We propose that veins develop at both vein and paravein boundaries in more \u2018primitive\u2019 insects, which have up to twice the number of veins present in Drosophila. We present a model in which different A/P boundaries organize vein-specific genetic programs to govern the development of individual veins.</jats:p>",
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