DOI: 10.1242/dev.124.7.1355. A novel family of T-box genes in urodele amphibian limb development and regeneration: candidate genes involved in vertebrate forelimb/hindlimb patterning

A novel family of T-box genes in urodele amphibian limb development and regeneration: candidate genes involved in vertebrate forelimb/hindlimb patterning

10.1242/dev.124.7.1355

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT In certain urodeles, a lost appendage, including hand and foot, can be completely replaced through epimorphic regeneration. The regeneration process involves cellular activities similar to those described for embryogenesis. Working on the assumption that the morphological pattern specific for a forelimb or a hindlimb is controlled by different gene activities in the two limbs, we employed a mRNA differential display screen for the detection of candidate limb identity genes. Using this approach, we have isolated a newt gene which in regenerating and developing limbs reveals properties expected of a gene having a role in controlling limb morphology: (1) it is exclusively expressed in the forelimbs, but not hindlimbs, (2) during embryonic development its expression is co-incident with forelimb bud formation, (3) it has an elevated message level throughout the undifferentiated limb bud and the blastema, respectively, and (4) it is expressed only in mesenchymal, but not in epidermal tissues. This novel newt gene shares a conserved DNA-binding domain, the T-box, with putative transcription factors including the Brachyury (T) gene product. In a following PCR-based screen, we used the evolutionarily conserved T-box motif and amplified a family of related genes in the newt; their different expression patterns in normal and regenerating forelimbs, hindlimbs and tail suggest, in general, an important role of T-domain proteins in vertebrate pattern formation.

Bibliography

Simon, H.-G., Kittappa, R., Khan, P. A., Tsilfidis, C., Liversage, R. A., & Oppenheimer, S. (1997). A novel family of T-box genes in urodele amphibian limb development and regeneration: candidate genes involved in vertebrate forelimb/hindlimb patterning. Development, 124(7), 1355â1366.

Authors 6

Hans-Georg Simon (first)
Raja Kittappa (additional)
Paul A. Khan (additional)
Catherine Tsilfidis (additional)
Richard A. Liversage (additional)
Simone Oppenheimer (additional)

References 46 Referenced 64

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Dates

Type	When
Created	4 years, 4 months ago (April 26, 2021, 12:07 a.m.)
Deposited	6 months, 3 weeks ago (Feb. 13, 2025, 9:33 p.m.)
Indexed	6 months, 3 weeks ago (Feb. 13, 2025, 10:10 p.m.)
Issued	28 years, 5 months ago (April 1, 1997)
Published	28 years, 5 months ago (April 1, 1997)
Published Online	28 years, 5 months ago (April 1, 1997)
Published Print	28 years, 5 months ago (April 1, 1997)

Funders 0

None

BibTeX

@article{Simon_1997, title={A novel family of T-box genes in urodele amphibian limb development and regeneration: candidate genes involved in vertebrate forelimb/hindlimb patterning}, volume={124}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.124.7.1355}, DOI={10.1242/dev.124.7.1355}, number={7}, journal={Development}, publisher={The Company of Biologists}, author={Simon, Hans-Georg and Kittappa, Raja and Khan, Paul A. and Tsilfidis, Catherine and Liversage, Richard A. and Oppenheimer, Simone}, year={1997}, month=apr, pages={1355–1366} }

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