DOI: 10.1242/dev.124.20.4039. The Drosophila gene Bearded encodes a novel small protein and shares 3′ UTR sequence motifs with multiple Enhancer of split Complex genes

The Drosophila gene Bearded encodes a novel small protein and shares 3′ UTR sequence motifs with multiple Enhancer of split Complex genes

10.1242/dev.124.20.4039

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT Gain-of-function alleles of the Drosophila gene Bearded (Brd) cause sensory organ multiplication and loss pheno-types indistinguishable at the cellular level from those caused by loss-of-function mutations in the genes of the Notch pathway (Leviten, M. W. and Posakony, J. W. (1996). Dev. Biol. 176, 264-283). We have carried out a molecular analysis of the structure and expression of both wild-type and mutant Brd transcription units. We find that the Brd transcript is truncated and accumulates to substantially higher levels in the gain-of-function mutants, due to the insertion of a transposable element of the blood family in the Brd 3′ untranslated region (UTR). The wild-type Brd 3′ UTR includes three copies of a 9-nucleotide sequence (CAGCTTTAA) that we refer to as the ‘Brd box’. Moreover, the 3′ UTRs of Brd and of the m4 transcription unit of the Enhancer of split gene complex [E(spl)-C] exhibit an unusually high degree of sequence identity that includes not only Brd box sequences but also a second motif we refer to as the ‘GY box’ (GTCTTCC). We find that both the Brd box and the GY box are also present in the 3′ UTRs of several basic helix-loop-helix repressor-encoding genes of the E(spl)-C, often in multiple copies, suggesting that a novel mode of post-transcriptional regulation applies to Brd and many E(spl)-C genes. The fact that the more abundant Brd mutant mRNA lacks the GY box and two of the Brd boxes present in wild-type Brd mRNA suggests that either or both of these elements may confer instability on transcripts that contain them. Finally, we find that Brd encodes a novel small protein of only 81 amino acids that is predicted to include a basic amphipathic α-helix. The deduced Brd protein shows sequence similarity to the E(spl)m4 protein, which is likewise expected to include a basic amphipathic α-helix, suggesting that the two proteins have related biochemical functions.

Bibliography

Leviten, M. W., Lai, E. C., & Posakony, J. W. (1997). The Drosophila gene Bearded encodes a novel small protein and shares 3â² UTR sequence motifs with multiple Enhancer of split Complex genes. Development, 124(20), 4039â4051.

Authors 3

Michael W. Leviten (first)
Eric C. Lai (additional)
James W. Posakony (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 11:01 p.m.)
Deposited	6 months, 2 weeks ago (Feb. 14, 2025, 12:09 a.m.)
Indexed	2 months ago (June 25, 2025, 10:09 p.m.)
Issued	27 years, 10 months ago (Oct. 15, 1997)
Published	27 years, 10 months ago (Oct. 15, 1997)
Published Online	27 years, 10 months ago (Oct. 15, 1997)
Published Print	27 years, 10 months ago (Oct. 15, 1997)

Funders 0

None

BibTeX

@article{Leviten_1997, title={The Drosophila gene Bearded encodes a novel small protein and shares 3′ UTR sequence motifs with multiple Enhancer of split Complex genes}, volume={124}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.124.20.4039}, DOI={10.1242/dev.124.20.4039}, number={20}, journal={Development}, publisher={The Company of Biologists}, author={Leviten, Michael W. and Lai, Eric C. and Posakony, James W.}, year={1997}, month=oct, pages={4039–4051} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n               <jats:p>Gain-of-function alleles of the Drosophila gene Bearded (Brd) cause sensory organ multiplication and loss pheno-types indistinguishable at the cellular level from those caused by loss-of-function mutations in the genes of the Notch pathway (Leviten, M. W. and Posakony, J. W. (1996). Dev. Biol. 176, 264-283). We have carried out a molecular analysis of the structure and expression of both wild-type and mutant Brd transcription units. We find that the Brd transcript is truncated and accumulates to substantially higher levels in the gain-of-function mutants, due to the insertion of a transposable element of the blood family in the Brd 3\u2032 untranslated region (UTR). The wild-type Brd 3\u2032 UTR includes three copies of a 9-nucleotide sequence (CAGCTTTAA) that we refer to as the \u2018Brd box\u2019. Moreover, the 3\u2032 UTRs of Brd and of the m4 transcription unit of the Enhancer of split gene complex [E(spl)-C] exhibit an unusually high degree of sequence identity that includes not only Brd box sequences but also a second motif we refer to as the \u2018GY box\u2019 (GTCTTCC). We find that both the Brd box and the GY box are also present in the 3\u2032 UTRs of several basic helix-loop-helix repressor-encoding genes of the E(spl)-C, often in multiple copies, suggesting that a novel mode of post-transcriptional regulation applies to Brd and many E(spl)-C genes. The fact that the more abundant Brd mutant mRNA lacks the GY box and two of the Brd boxes present in wild-type Brd mRNA suggests that either or both of these elements may confer instability on transcripts that contain them. Finally, we find that Brd encodes a novel small protein of only 81 amino acids that is predicted to include a basic amphipathic \u03b1-helix. The deduced Brd protein shows sequence similarity to the E(spl)m4 protein, which is likewise expected to include a basic amphipathic \u03b1-helix, suggesting that the two proteins have related biochemical functions.</jats:p>",
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