In vitro lens transdifferentiation of Xenopus laevis outer cornea induced by Fibroblast Growth Factor (FGF)
10.1242/dev.124.2.421
Crossref journal-article
The Company of Biologists
Development (237)
Abstract

ABSTRACT It has been shown that lens regeneration from outer cornea of larval Xenopus laevisis dependent on neural retina both in vivo and in tissue culture. The isolated outer cornea cultured in the presence of bovine brain-derived acidic Fibroblast Growth Factor (aFGF) is able to reprogram the differentiation into lens fibers, although this transdifferentiative process is not coupled with the formation of a normally organized lens. The capacity of aFGF to promote lens differentiation from cornea is not linked to its mitogenic activity. The cultured corneal cells can transdifferentiate into lens fibers in the presence of aFGF when DNA replication and cell proliferation are prevented by addition of aphidicolin, a specific inhibitor of DNA polymerase in eukaryotes, to the culture medium.

Bibliography

Bosco, L., Venturini, G., & Willems, D. (1997). In vitro lens transdifferentiation of Xenopus laevis outer cornea induced by Fibroblast Growth Factor (FGF). Development, 124(2), 421–428.

Authors 3
  1. Luigi Bosco (first)
  2. Giorgio Venturini (additional)
  3. Daniela Willems (additional)
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Dates
Type When
Created 4 years, 4 months ago (April 25, 2021, 11:04 p.m.)
Deposited 6 months, 2 weeks ago (Feb. 13, 2025, 7:19 p.m.)
Indexed 6 months, 2 weeks ago (Feb. 14, 2025, 12:34 a.m.)
Issued 28 years, 7 months ago (Jan. 15, 1997)
Published 28 years, 7 months ago (Jan. 15, 1997)
Published Online 28 years, 7 months ago (Jan. 15, 1997)
Published Print 28 years, 7 months ago (Jan. 15, 1997)
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@article{Bosco_1997, title={In vitro lens transdifferentiation of Xenopus laevis outer cornea induced by Fibroblast Growth Factor (FGF)}, volume={124}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.124.2.421}, DOI={10.1242/dev.124.2.421}, number={2}, journal={Development}, publisher={The Company of Biologists}, author={Bosco, Luigi and Venturini, Giorgio and Willems, Daniela}, year={1997}, month=jan, pages={421–428} }